Detection of Protein Biomarkers Relevant to Sperm Characteristics and Fertility in Semen in Three Wild Felidae: The Flat-Headed Cat (Prionailurus planiceps), Fishing Cat (Prionailurus viverrinus), and Asiatic Golden Cat (Catopuma temminckii)
Abstract
:Simple Summary
Abstract
1. Introduction
2. Material and Methods
2.1. Animals
2.2. Anesthesia and Morphometric Measurements
2.3. Semen Collection and Evaluation
2.4. Western Immunoblotting and Evaluation
2.5. Sperm Immunofluorescence Microscopy
2.6. Statistical Analyses
3. Results
3.1. Differences in Ejaculated Semen Characteristics and Sperm Traits among Three Wild Cat Species
3.2. Expression of IZUMO1, CRISP2, and CRISP3 Proteins in Spermatozoa and Seminal Plasma among Three Wild Cat Species
3.3. Correlation between IZUMO1, CRISP2, and CRISP3 Protein Expression Levels and Ejaculate Characteristics among Three Wildcat Species
3.4. Percentage of Cleaved Caspase-3-Positive Sperm and Its Correlation with Semen Characteristics among Three Wildcat Species
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Parameters | IZUMO1 | CRISP2 | CRISP3 | |||
---|---|---|---|---|---|---|
Sperm Pellet | Seminal Plasma | Sperm Pellet | Seminal Plasma | Sperm Pellet | Seminal Plasma | |
Semen volume (µL) | 0.229 (p = 0.361) | −0.004 (p = 0.987) | 0.253 (p = 0.309) | 0.385 (p = 0.085) | 0.536 (p < 0.05) | 0.197 (p = 0.393) |
Sperm concentration (spermatozoa/mL) | 0.492 (p < 0.05) | 0.495 (p < 0.05) | 0.198 (p = 0.432) | 0.275 (p = 0.227) | 0.479 (p < 0.05) | 0.137 (p = 0.563) |
Total sperm number (spermatozoa) | 0.492 (p < 0.05) | 0.495 (p < 0.05) | 0.198 (p = 0.432) | 0.275 (p = 0.227) | 0.479 (p < 0.05) | 0.137 (p = 0.563) |
Motility (%) | 0.582 (p < 0.01) | 0.596 (p < 0.01) | 0.500 (p < 0.05) | 0.367 (p = 0.102) | 0.280 (p = 0.185) | 0.175 (p = 0.447) |
Viability (%) | 0.441 (p < 0.05) | 0.484 (p < 0.05) | 0.615 (p < 0.01) | 0.397 (p = 0.075) | 0.402 (p = 0.052) | 0.291 (p = 0.201) |
Intact acrosome (%) | 0.221 (p = 0.3) | 0.739 (p < 0.01) | 0.355 (p = 0.089) | 0.503 (p < 0.05) | 0.047 (p = 0.826) | 0.137 (p = 0.563) |
Normal sperm membrane (%) | 0.566 (p < 0.01) | 0.620 (p < 0.01) | −0.057 (p = 0.681) | 0.004 (p = 0.975) | 0.372 (p < 0.01) | 0.037 (p = 0.775) |
Normal sperm morphology (%) | 0.524 (p < 0.05) | 0.389 (p = 0.081) | −0.028 (p = 0.912) | 0.279 (p = 0.220) | 0.367 (p = 0.134) | 0.090 (p = 0.697) |
Normal DNA (%) | 0.254 (p = 0.266) | 0.548 (p < 0.05) | 0.572 (p < 0.01) | 0.548 (p < 0.05) | 0.286 (p = 0.209) | 0.153 (p = 0.508) |
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Wittayarat, M.; Kiatsomboon, S.; Kupthammasan, N.; Tipkantha, W.; Yimprasert, S.; Thongphakdee, A.; Panyaboriban, S. Detection of Protein Biomarkers Relevant to Sperm Characteristics and Fertility in Semen in Three Wild Felidae: The Flat-Headed Cat (Prionailurus planiceps), Fishing Cat (Prionailurus viverrinus), and Asiatic Golden Cat (Catopuma temminckii). Animals 2024, 14, 1027. https://doi.org/10.3390/ani14071027
Wittayarat M, Kiatsomboon S, Kupthammasan N, Tipkantha W, Yimprasert S, Thongphakdee A, Panyaboriban S. Detection of Protein Biomarkers Relevant to Sperm Characteristics and Fertility in Semen in Three Wild Felidae: The Flat-Headed Cat (Prionailurus planiceps), Fishing Cat (Prionailurus viverrinus), and Asiatic Golden Cat (Catopuma temminckii). Animals. 2024; 14(7):1027. https://doi.org/10.3390/ani14071027
Chicago/Turabian StyleWittayarat, Manita, Supalak Kiatsomboon, Navapol Kupthammasan, Wanlaya Tipkantha, Surasak Yimprasert, Ampika Thongphakdee, and Saritvich Panyaboriban. 2024. "Detection of Protein Biomarkers Relevant to Sperm Characteristics and Fertility in Semen in Three Wild Felidae: The Flat-Headed Cat (Prionailurus planiceps), Fishing Cat (Prionailurus viverrinus), and Asiatic Golden Cat (Catopuma temminckii)" Animals 14, no. 7: 1027. https://doi.org/10.3390/ani14071027
APA StyleWittayarat, M., Kiatsomboon, S., Kupthammasan, N., Tipkantha, W., Yimprasert, S., Thongphakdee, A., & Panyaboriban, S. (2024). Detection of Protein Biomarkers Relevant to Sperm Characteristics and Fertility in Semen in Three Wild Felidae: The Flat-Headed Cat (Prionailurus planiceps), Fishing Cat (Prionailurus viverrinus), and Asiatic Golden Cat (Catopuma temminckii). Animals, 14(7), 1027. https://doi.org/10.3390/ani14071027