Isolation and Genomic Characterization of a Chinese Genotype C Bovine Parainfluenza Virus Type 3 from Cattle and Its Pathogenicity in C57BL/6 Mice
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Clinical Samples and Etiological Examinations
2.2. Virus Isolation
2.3. Immunofluorescence Test
2.4. Electron Microscopy
2.5. Illumina Sequencing
2.6. Genomic Characterization and Phylogenetic Analysis
2.7. Animal Experiments
3. Results
3.1. BPIV-3 Detection, Isolation, and Identification
3.2. Pathogenicity of BPIV-3 SC in C57BL/6 Mice
3.3. Whole Genome Sequencing and Phylogenetic Analysis of BPIV-3
3.4. Genomic Characteristics of BPIV-3 SC
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Pathogens | Sequence (5′-3′) | Length of Amplicon (bp) | References |
---|---|---|---|
BVDV | GGTAGCAACAGTGGTGAGTTC | 130 | [21] |
CTCAGGTTAAGATGTGCTGTG | |||
BCoV | ACGTTCTTTTAAAACAGCCGATG | 409 | [22] |
TGCCAGAACAAGACTAGCAA | |||
BRSV | TATGCTATGTCCCGATTGG | 600 | [21] |
ACTGATTTGGCTAGTACACCC | |||
BRV | GGTAGCGGCGTTATTTCC | 407 | [23] |
CGCCATCTGAGTGATTACTC | |||
BPIV-3 | GCATCACAAACTCCGCAATAT | 1048 | [17] |
TGCTTGATTTTTCCGACTCCT | |||
BADV-3 | CTCCTGGGTCCTGGCCTTAGTT | 1182 | [24] |
AGTGTTTGTGGGTAAAGGGCAATAG | |||
BHV-1 | GCTCGCCAACTTCTTTCAGGG | 306 | [21] |
GCGTCAAACTCCTCCTCTTCCTC |
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Chen, J.; Qiu, Y.; Xiong, P.; Wang, Z.; Li, N.; Ye, C.; Peng, Y. Isolation and Genomic Characterization of a Chinese Genotype C Bovine Parainfluenza Virus Type 3 from Cattle and Its Pathogenicity in C57BL/6 Mice. Animals 2024, 14, 463. https://doi.org/10.3390/ani14030463
Chen J, Qiu Y, Xiong P, Wang Z, Li N, Ye C, Peng Y. Isolation and Genomic Characterization of a Chinese Genotype C Bovine Parainfluenza Virus Type 3 from Cattle and Its Pathogenicity in C57BL/6 Mice. Animals. 2024; 14(3):463. https://doi.org/10.3390/ani14030463
Chicago/Turabian StyleChen, Jing, Yangyang Qiu, Pan Xiong, Zhijie Wang, Nengzhang Li, Chao Ye, and Yuanyi Peng. 2024. "Isolation and Genomic Characterization of a Chinese Genotype C Bovine Parainfluenza Virus Type 3 from Cattle and Its Pathogenicity in C57BL/6 Mice" Animals 14, no. 3: 463. https://doi.org/10.3390/ani14030463
APA StyleChen, J., Qiu, Y., Xiong, P., Wang, Z., Li, N., Ye, C., & Peng, Y. (2024). Isolation and Genomic Characterization of a Chinese Genotype C Bovine Parainfluenza Virus Type 3 from Cattle and Its Pathogenicity in C57BL/6 Mice. Animals, 14(3), 463. https://doi.org/10.3390/ani14030463