Expression of IkappaB Family in the Ovine Liver during Early Pregnancy
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animal Tissue Collection
2.2. RT-qPCR Assay
2.3. Western Blot
2.4. Immunohistochemistry Analysis
2.5. Statistical Analysis
3. Results
3.1. Gene Expression of IκB Family in the Liver
3.2. Protein Expression of IκB Family in the Livers
3.3. Immunohistochemistry for IκBβ and IKKγ Proteins in the Livers
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | Primer | Sequence | Size (bp) | Accession Numbers |
---|---|---|---|---|
BCL-3 | Forward | GCGACCAGAGGCAATTTACTACCAG | 98 | XM_027978453.2 |
Reverse | GAGGTGTAGGCAAGTTCAGCAGAG | |||
NFKBIA | Forward | AGGACGAGGAGTATGAGCAGATGG | 130 | NM_001166184.1 |
Reverse | GCCAAGTGCAGGAACGAGTCTC | |||
NFKBIB | Forward | CCCCAAGACCTACCTCGCTCAG | 119 | XM_027978262.2 |
Reverse | TCCAGTCCTCTTCACTCTCATCCTC | |||
NFKBIE | Forward | GCACTCACGTACATTTCCGAGGAC | 97 | XM_042236979.1 |
Reverse | GCAGCAGAGCCAGGCAATACAG | |||
IKBKG | Forward | GGGCAACCAGAGGGAGGAGAAG | 146 | XM_027963334.2 |
Reverse | GGCATGTCTTCAGGCGTTCCAC | |||
NFKBIZ | Forward | GCAAAGGCGTACAATGGAAACACC | 137 | NM_001306117.1 |
Reverse | GGCTGCTCGTTCTCCAAGTTCC | |||
NFKBID | Forward | ACATTCGTGAGCATAAGGGCAAGAC | 114 | XM_027977435.2 |
Reverse | GATGGTCAGTGGCATTGGGTTCC | |||
GAPDH | Forward | GGGTCATCATCTCTGCACCT | 176 | NM_001190390.1 |
Reverse | GGTCATAAGTCCCTCCACGA |
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Cai, C.; Ren, Y.; Cao, J.; Fang, S.; Zhang, L.; Yang, L. Expression of IkappaB Family in the Ovine Liver during Early Pregnancy. Animals 2023, 13, 1057. https://doi.org/10.3390/ani13061057
Cai C, Ren Y, Cao J, Fang S, Zhang L, Yang L. Expression of IkappaB Family in the Ovine Liver during Early Pregnancy. Animals. 2023; 13(6):1057. https://doi.org/10.3390/ani13061057
Chicago/Turabian StyleCai, Chunjiang, Ying Ren, Jianhua Cao, Shengya Fang, Leying Zhang, and Ling Yang. 2023. "Expression of IkappaB Family in the Ovine Liver during Early Pregnancy" Animals 13, no. 6: 1057. https://doi.org/10.3390/ani13061057
APA StyleCai, C., Ren, Y., Cao, J., Fang, S., Zhang, L., & Yang, L. (2023). Expression of IkappaB Family in the Ovine Liver during Early Pregnancy. Animals, 13(6), 1057. https://doi.org/10.3390/ani13061057