Beneficial Shifts in the Gut Bacterial Community of Gilthead Seabream (Sparus aurata) Juveniles Supplemented with Allium-Derived Compound Propyl Propane Thiosulfonate (PTSO)
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Allium-Based Product
2.2. Animals, Experimental Design, and Sample Collection
2.3. DNA Extraction
2.4. 16S rRNA Gene High-Throughput Sequencing
2.5. Sequences Processing and Data Analysis
2.6. Statistics
3. Results
3.1. Changes in Bacterial Community Composition
3.2. Effect of Allium-Derived PTSO Supplementation on Alpha and Beta Diversity Indices
3.3. Effect of Allium-Derived PTSO Supplementation on the Body Weight of Gilthead Seabream Juveniles
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Explanatory Variables | D.f | F | p | |
---|---|---|---|---|
Bacterial ASV richness | Treatment | 1188 | 3.35 | 0.386 |
Gut Region | 1188 | 3.20 | 0.075 | |
Tank (Treatment) | 4188 | 3.56 | 0.008 | |
Gut Region*Treatment | 1188 | 0.18 | 0.668 | |
Shannon diversity index | Treatment | 1188 | 20.23 | 0.009 |
Gut Region | 1188 | 6.67 | 0.011 | |
Tank (Treatment) | 4188 | 0.89 | 0.469 | |
Gut Region*Treatment | 1188 | 0.91 | 0.341 |
β-Diversity Distance Matrix | Explanatory Variables | D.f. | Pseudo-F | p | |
---|---|---|---|---|---|
Both gut regions | Weighted UniFrac | Treatment | 1195 | 8.13 | 0.092 |
Gut region | 1195 | 5.13 | 0.005 | ||
Tank (Treatment) | 4195 | 3.31 | 0.002 | ||
Treatment*Gut region | 1195 | 0.44 | 0.702 | ||
Unweighted UniFrac | Treatment | 1195 | 2.22 | 0.088 | |
Gut region | 1195 | 1.65 | 0.031 | ||
Tank (Treatment) | 4195 | 1.83 | 0.001 | ||
Treatment*Gut region | 1195 | 0.83 | 0.745 | ||
Foregut | Weighted UniFrac | Treatment | 1195 | 5.26 | 0.082 |
Tank (Treatment) | 4195 | 2.03 | 0.016 | ||
Unweighted UniFrac | Treatment | 1195 | 1.55 | 0.189 | |
Tank (Treatment) | 4195 | 1.38 | 0.011 | ||
Hindgut | Weighted UniFrac | Treatment | 1195 | 7.82 | 0.074 |
Tank (Treatment) | 4195 | 2.27 | 0.025 | ||
Unweighted UniFrac | Treatment | 1195 | 2.15 | 0.021 | |
Tank (Treatment) | 4195 | 1.28 | 0.041 |
Sampling Time | Control | Allium-Derived PTSO | Independent Variables | F | D.f. | p |
---|---|---|---|---|---|---|
Mean BW Whole experiment | 158.33 ± 5.65 | 153.84 ± 5.46 | Treatment | 1.74 | 1454 | 0.188 |
Tank (treatment) | 1.12 | 4454 | 0.346 | |||
Sampling time Treatment*sampling time | 5438.96 1.85 | 1454 1454 | <0.001 0.174 | |||
BW Week 12 | 385.37 ± 3.49 | 376.32 ± 3.50 | Treatment | 3.57 | 1.30 | 0.057 |
Tank (Treatment) | 0.35 | 4.30 | 0.772 |
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Rabelo-Ruiz, M.; Newman-Portela, A.M.; Peralta-Sánchez, J.M.; Martín-Platero, A.M.; Agraso, M.d.M.; Bermúdez, L.; Aguinaga, M.A.; Baños, A.; Maqueda, M.; Valdivia, E.; et al. Beneficial Shifts in the Gut Bacterial Community of Gilthead Seabream (Sparus aurata) Juveniles Supplemented with Allium-Derived Compound Propyl Propane Thiosulfonate (PTSO). Animals 2022, 12, 1821. https://doi.org/10.3390/ani12141821
Rabelo-Ruiz M, Newman-Portela AM, Peralta-Sánchez JM, Martín-Platero AM, Agraso MdM, Bermúdez L, Aguinaga MA, Baños A, Maqueda M, Valdivia E, et al. Beneficial Shifts in the Gut Bacterial Community of Gilthead Seabream (Sparus aurata) Juveniles Supplemented with Allium-Derived Compound Propyl Propane Thiosulfonate (PTSO). Animals. 2022; 12(14):1821. https://doi.org/10.3390/ani12141821
Chicago/Turabian StyleRabelo-Ruiz, Miguel, Antonio M. Newman-Portela, Juan Manuel Peralta-Sánchez, Antonio Manuel Martín-Platero, María del Mar Agraso, Laura Bermúdez, María Arántzazu Aguinaga, Alberto Baños, Mercedes Maqueda, Eva Valdivia, and et al. 2022. "Beneficial Shifts in the Gut Bacterial Community of Gilthead Seabream (Sparus aurata) Juveniles Supplemented with Allium-Derived Compound Propyl Propane Thiosulfonate (PTSO)" Animals 12, no. 14: 1821. https://doi.org/10.3390/ani12141821
APA StyleRabelo-Ruiz, M., Newman-Portela, A. M., Peralta-Sánchez, J. M., Martín-Platero, A. M., Agraso, M. d. M., Bermúdez, L., Aguinaga, M. A., Baños, A., Maqueda, M., Valdivia, E., & Martínez-Bueno, M. (2022). Beneficial Shifts in the Gut Bacterial Community of Gilthead Seabream (Sparus aurata) Juveniles Supplemented with Allium-Derived Compound Propyl Propane Thiosulfonate (PTSO). Animals, 12(14), 1821. https://doi.org/10.3390/ani12141821