Immune Modulation Ability of Hepcidin from Teleost Fish
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Peptide Synthesis
2.2. Cell Culture and Incubation with Hep20
2.3. Experiment Fish and Hep20 Inoculation and Sample Collection
2.4. RNA Extraction and RT-qPCR
2.5. In Silico Analysis of the Structure of Hep20
2.6. Statistical Analysis
3. Results
3.1. Sequence and Structure Analysis of Fish Hepcidin
3.2. Cytokine Expression Profile in the Anterior Intestine of European Sea Bass Injected with Hep20
3.3. Cytokine Expression Profile in the Spleen of European Sea Bass Injected with Hep20
3.4. Cytokine Expression Profile in the Head Kidney of European Sea Bass Injected with Hep20
3.5. Cytokine Expression Induced by Hep20 in Cell Culture
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Álvarez, C.A.; Santana, P.A.; Salinas-Parra, N.; Beltrán, D.; Guzmán, F.; Vega, B.; Acosta, F.; Mercado, L. Immune Modulation Ability of Hepcidin from Teleost Fish. Animals 2022, 12, 1586. https://doi.org/10.3390/ani12121586
Álvarez CA, Santana PA, Salinas-Parra N, Beltrán D, Guzmán F, Vega B, Acosta F, Mercado L. Immune Modulation Ability of Hepcidin from Teleost Fish. Animals. 2022; 12(12):1586. https://doi.org/10.3390/ani12121586
Chicago/Turabian StyleÁlvarez, Claudio Andrés, Paula A. Santana, Nicolás Salinas-Parra, Dina Beltrán, Fanny Guzmán, Belinda Vega, Félix Acosta, and Luis Mercado. 2022. "Immune Modulation Ability of Hepcidin from Teleost Fish" Animals 12, no. 12: 1586. https://doi.org/10.3390/ani12121586