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Communication

Application of the FISH Technique to Visualize Sex Chromosomes in Domestic Cat Spermatozoa

1
Department of Animal Reproduction, Anatomy and Genomics, University of Agriculture, Mickiewicza 24/28, 30-059 Krakow, Poland
2
Veterinary Research Institute, Hudcova 296/70, 621 00 Brno, Czech Republic
3
Department of Reproduction and Clinic of Farm Animals, Wrocław University of Environmental and Life Sciences, Grunwaldzki Square 49, 50-366 Wrocław, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Sabine Schäfer-Somi
Animals 2021, 11(7), 2106; https://doi.org/10.3390/ani11072106
Received: 15 June 2021 / Revised: 9 July 2021 / Accepted: 14 July 2021 / Published: 15 July 2021
(This article belongs to the Special Issue Biotechnology and Reproduction in Companion Animals)
The study was conducted with the aim of visualizing sex chromosomes in domestic cat sperm. Our research was carried out using fluorescence in situ hybridization (FISH) technique and the microscopic analysis showed the presence of X and Y chromosomes in domestic cat spermatozoa. The procedure used for sperm decondensation and FISH is adequate to visualize chromosomes in domestic cat sperm.
Fluorescence in situ hybridization is a molecular cytogenetics technique that enables the visualization of chromosomes in cells via fluorescently labeled molecular probes specific to selected chromosomes. Despite difficulties in carrying out the FISH technique on sperm, related to the need for proper nuclear chromatin decondensation, this technique has already been used to visualize chromosomes in human, mouse, cattle, swine, horse, and dog spermatozoa. Until now, FISH has not been performed on domestic cat sperm; therefore, the aim of this study was to visualize sex chromosomes in domestic cat sperm. The results showed the presence of X and Y chromosomes in feline spermatozoa. The procedure used for sperm decondensation and fluorescence in situ hybridization was adequate to visualize chromosomes in domestic cat spermatozoa and, in the future, it may be used to determine the degree of chromosomal abnormalities in these gametes. View Full-Text
Keywords: FISH; cat spermatozoa; WCPP FISH; cat spermatozoa; WCPP
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MDPI and ACS Style

Kij-Mitka, B.; Cernohorska, H.; Kubickova, S.; Prochowska, S.; Niżański, W.; Kochan, J.; Bugno-Poniewierska, M. Application of the FISH Technique to Visualize Sex Chromosomes in Domestic Cat Spermatozoa. Animals 2021, 11, 2106. https://doi.org/10.3390/ani11072106

AMA Style

Kij-Mitka B, Cernohorska H, Kubickova S, Prochowska S, Niżański W, Kochan J, Bugno-Poniewierska M. Application of the FISH Technique to Visualize Sex Chromosomes in Domestic Cat Spermatozoa. Animals. 2021; 11(7):2106. https://doi.org/10.3390/ani11072106

Chicago/Turabian Style

Kij-Mitka, Barbara, Halina Cernohorska, Svatava Kubickova, Sylwia Prochowska, Wojciech Niżański, Joanna Kochan, and Monika Bugno-Poniewierska. 2021. "Application of the FISH Technique to Visualize Sex Chromosomes in Domestic Cat Spermatozoa" Animals 11, no. 7: 2106. https://doi.org/10.3390/ani11072106

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