Influence of Infectious Pancreatic Necrosis Virus and Yersinia ruckeri Co-Infection on a Non-Specific Immune System in Rainbow Trout (Oncorhynchus mykiss)
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Fish
2.2. Experimental Infection
- group C—uninfected control;
- group Yer—infected with Y. ruckeri;
- group IPN—infected with IPNV;
- group Yer+IPN—co-infected with Y. ruckeri and IPNV.
2.2.1. Experiment I
2.2.2. Experiment II
2.2.3. Sampling
2.3. Immunological Tests
2.3.1. Tests Performed on Blood Serum
Total Protein
Gamma Globulin Level
Lysozyme Activity
Ceruloplasmin Activity
- acetate buffer (pH 5.2 containing crystalline acetic acid, sodium acetate trihydrate, and 15 mg EDTA);
- buffered substrate solution (0.2% p-phenylenediamine (PPD) in acetic buffer);
- sodium azide solution (0.02% sodium azide solution in deionized water).
2.3.2. Tests Performed on Tissue
- nitro blue tetrazolium (NBT)—0.1% NBT (Sigma-Aldrich) to the first two rows (two replicates);
- RBA—PMA solution with 0.1% NBT to the next three rows (three replicates);
- PKA—bacterial suspension and 0.1% NBT to the last three rows (triplicate).
2.4. Statistical Analysis
3. Results
3.1. Experiment I
3.2. Experiment II
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Pajdak-Czaus, J.; Schulz, P.; Terech-Majewska, E.; Szweda, W.; Siwicki, A.K.; Platt-Samoraj, A. Influence of Infectious Pancreatic Necrosis Virus and Yersinia ruckeri Co-Infection on a Non-Specific Immune System in Rainbow Trout (Oncorhynchus mykiss). Animals 2021, 11, 1974. https://doi.org/10.3390/ani11071974
Pajdak-Czaus J, Schulz P, Terech-Majewska E, Szweda W, Siwicki AK, Platt-Samoraj A. Influence of Infectious Pancreatic Necrosis Virus and Yersinia ruckeri Co-Infection on a Non-Specific Immune System in Rainbow Trout (Oncorhynchus mykiss). Animals. 2021; 11(7):1974. https://doi.org/10.3390/ani11071974
Chicago/Turabian StylePajdak-Czaus, Joanna, Patrycja Schulz, Elżbieta Terech-Majewska, Wojciech Szweda, Andrzej Krzysztof Siwicki, and Aleksandra Platt-Samoraj. 2021. "Influence of Infectious Pancreatic Necrosis Virus and Yersinia ruckeri Co-Infection on a Non-Specific Immune System in Rainbow Trout (Oncorhynchus mykiss)" Animals 11, no. 7: 1974. https://doi.org/10.3390/ani11071974
APA StylePajdak-Czaus, J., Schulz, P., Terech-Majewska, E., Szweda, W., Siwicki, A. K., & Platt-Samoraj, A. (2021). Influence of Infectious Pancreatic Necrosis Virus and Yersinia ruckeri Co-Infection on a Non-Specific Immune System in Rainbow Trout (Oncorhynchus mykiss). Animals, 11(7), 1974. https://doi.org/10.3390/ani11071974