Association of IL-4 and IL-4R Polymorphisms with Litter Size Traits in Pigs
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals and DNA Extraction
2.2. Verification of Porcine IL-4 and IL-4R Polymorphisms and Genotyping
2.3. Statistical Analysis
3. Results
3.1. Polymorphisms of Porcine IL-4 and IL-4R Genes
3.2. Genotypic and Allelic Frequencies
3.3. Associations of Porcine IL-4 and IL-4R Polymorphisms with Litter Size Traits
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Primers | Location | Primer Sequences | PCR Size (bp) | Ta (°C) |
---|---|---|---|---|
IL-4-1 | Exon 1 | F: 5′-GTTAGCTCCTCCCAGTAAAC-3′ R: 5′-CATCAGAGCATCTGGAGAGA-3′ | 236 | 60 |
IL-4-2 | Exon 2 | F: 5′-AACGCTCTCTCTTGCTCTCT-3′ R: 5′-TGCCTCCTGTATTCCAGGTA-3′ | 409 | 60 |
IL-4-3 | Exon 3 | F: 5′-ACACGGTAATTGGTGGCTCT-3′ R: 5′-TGTTAGAAGCCAAGCTGTGG-3′ | 189 | 60 |
IL-4-4 | Exon 4 | F: 5′-AGGGAGAAAGGCATGAGCAA-3′ R: 5′-GCCTATGCCATTAAAGTGACA-3′ | 227 | 60 |
IL-4-5 | Intron 2 | F: 5′-GTACACGCCCTCTTCTAAGA-3′ R: 5′-TCCCTGGAAATCTCACATGC-3′ | 308 | 60 |
IL-4-6 | Intron 3 | F: 5′-GTGGTAGGTATCCTTTCCAC-3′ R: 5′-AACAGTGATCAAACCAGGGC-3′ | 333 | 58 |
SNP Position (SNP ID) | Location | Primer Sequences | PCR Size (bp) | Ta (°C) | Restriction Enzyme |
---|---|---|---|---|---|
IL-4 g.134993898T > C (rs329453960) | Intron 3 | F: 5′-GTGGTAGGTATCCTTTCCAC-3′ R: 5′-AACAGTGATCAAACCAGGGC-3′ | 333 | 58 | BsuRI |
IL-4R c.163G > A (rs342744061) | Exon 1 | F: 5′-TCTTGATCACTGGGCTTCCG-3′ R: 5′-AACTCAGCGCTGCAGTTGAC-3′ | 152 | 60 | PflFI |
IL-4R c.242C > T (rs334778260) | Exon 2 | F: 5′-GGTCACATGACCAGCCTAAT-3′ R: 5′-TTGAAGGAGCTGTTCCACAG-3′ | 180 | 60 | AflIII |
IL-4R c.623G > A (rs790596006) | Exon 4 | F: 5′-TCTATAACGTGACCTACCTG-3′ R: 5′-TTAAGCCACTTGACACTCGG-3′ | 148 | 60 | MluI |
IL-4R c.1016G > T (rs692527061) | Exon 8 | F: 5′-GCTGGAAGACTTGTCTTACC-3′ R: 5′-GATCGTCTTGCTGACCTCTA-3′ | 149 | 60 | BsuRI |
IL-4R c.1577A > T (rs342791614) | Exon 8 | F: 5′-CTGGACTCGGACCCAGAG-3′ R: 5′-ACACTCTGGCGCAGGATCT-3′ | 137 | 58 | AluI |
SNPs | n | Genotypic Frequencies | Allelic Frequencies 1 | p-Value 2 (χ2) | |||
---|---|---|---|---|---|---|---|
AA | AB | BB | A | B | |||
IL-4 g.134993898T > C | 320 | 0.41 | 0.47 | 0.12 | 0.65 | 0.35 | 0.90 |
IL-4R c.1577A > T | 318 | 0.28 | 0.55 | 0.17 | 0.56 | 0.44 | 0.10 |
Parity | Traits 1 | Genotypes (Means ± SE) 2 | Additive | Dominance | ||
---|---|---|---|---|---|---|
TT | TC | CC | ||||
First parity | n | 132 | 150 | 38 | ||
TNB | 9.58 ± 0.45 | 9.77 ± 0.48 | 9.68 ± 0.67 | −0.05 ± 0.25 | 0.14 ± 0.32 | |
NBA | 8.65 ± 0.47 | 8.57 ± 0.36 | 8.08 ± 0.42 | 0.29 ± 0.27 | 0.11 ± 0.12 | |
NWA | 8.15 ± 0.47 | 7.85 ± 0.64 | 7.28 ± 0.54 | 0.44 ± 0.22 | 0.14 ± 0.12 | |
MBW | 1.58 ± 0.06 | 1.60 ± 0.08 | 1.67 ± 0.08 | −0.04 ± 0.02 | −0.02 ± 0.02 | |
MWW | 6.42 ± 0.12 | 6.58 ± 0.15 | 6.52 ± 0.18 | −0.05 ± 0.05 | 0.11 ± 0.08 | |
Later parities (2nd–8th parities) | n | 348 | 380 | 97 | ||
TNB | 11.25 ± 0.51 | 11.03 ± 0.53 | 10.72 ± 0.55 | 0.27 ± 0.28 | 0.04 ± 0.17 | |
NBA | 10.48 ± 0.58 | 9.92 ± 0.57 | 9.47 ± 0.75 | 0.51 ± 0.32 | 0.06 ± 0.32 | |
NWA | 9.72 ± 0.47 b | 9.42 ± 0.47 b | 8.45 ± 0.52 a | 0.64 ± 0.21 * | 0.33 ± 0.34 | |
MBW | 1.58 ± 0.05 | 1.57 ± 0.04 | 1.54 ± 0.05 | 0.02 ± 0.01 | 0.01 ± 0.02 | |
MWW | 6.62 ± 0.05 | 6.67 ± 0.05 | 6.59 ± 0.07 | 0.02 ± 0.02 | 0.06 ± 0.02 |
Parity | Traits 1 | Genotypes (Means ± SE) 2 | Additive | Dominance | ||
---|---|---|---|---|---|---|
AA | AT | TT | ||||
First parity | n | 89 | 176 | 53 | ||
TNB | 9.48 ± 0.38 | 10.03 ± 0.76 | 9.27 ± 0.58 | 0.11 ± 0.32 | 0.65 ± 0.35 | |
NBA | 8.25 ± 0.37 | 8.55 ± 0.52 | 8.19 ± 0.67 | 0.03 ± 0.32 | 0.33 ± 0.47 | |
NWA | 7.45 ± 0.53 | 7.57 ± 0.61 | 7.30 ± 0.49 | 0.08 ± 0.35 | 0.20 ± 0.42 | |
MBW | 1.54 ± 0.04 | 1.58 ± 0.08 | 1.67 ± 0.08 | −0.06 ± 0.02 | −0.02 ± 0.03 | |
MWW | 6.42 ± 0.12 | 6.39 ± 0.10 | 6.55 ± 0.18 | −0.06 ± 0.05 | −0.09 ± 0.07 | |
Later parities (2nd–8th parities) | n | 235 | 449 | 133 | ||
TNB | 10.51 ± 0.49 | 10.55 ± 0.43 | 11.54 ± 0.68 | −0.52 ± 0.28 | −0.47 ± 0.32 | |
NBA | 9.21 ± 0.56 a | 9.43 ± 0.47 a | 10.71 ± 0.64 b | −0.75 ± 0.28 * | −0.53 ± 0.36 | |
NWA | 8.61 ± 0.52 a | 9.09 ± 0.46 a | 10.20 ± 0.58 b | −0.80 ± 0.28 ** | −0.32 ± 0.31 | |
MBW | 1.55 ± 0.05 | 1.60 ± 0.04 | 1.57 ± 0.05 | −0.01 ± 0.02 | 0.04 ± 0.02 | |
MWW | 6.62 ± 0.06 | 6.63 ± 0.05 | 6.60 ± 0.06 | 0.01 ± 0.02 | 0.02 ± 0.03 |
Parity | Traits 1 | Number of Favorable Alleles (Means ± SE) 2 | ||||
---|---|---|---|---|---|---|
0 | 1 | 2 | 3 | 4 | ||
First parity | n | 7 | 59 | 144 | 89 | 18 |
TNB | 10.72 ± 1.42 | 9.58 ± 0.57 | 10.12 ± 0.54 | 10.15 ± 0.52 | 8.68 ± 0.72 | |
NBA | 8.84 ± 1.25 | 8.24 ± 0.58 | 8.74 ± 0.52 | 9.15 ± 0.67 | 8.12 ± 0.95 | |
NWA | 7.23 ± 1.27 | 7.25 ± 0.73 | 7.88 ± 0.64 | 8.57 ± 0.71 | 7.82 ± 0.84 | |
MBW | 1.48 ± 0.16 | 1.58 ± 0.05 | 1.57 ± 0.04 | 1.56 ± 0.05 | 1.58 ± 0.08 | |
MWW | 6.52 ± 0.32 | 6.41 ± 0.12 | 6.55 ± 0.10 | 6.43 ± 0.08 | 6.64 ± 0.19 | |
Later parities (2nd–8th parities) | n | 18 | 152 | 371 | 225 | 48 |
TNB | 9.94 ± 1.14 | 10.21 ± 0.62 | 10.87 ± 0.51 | 10.68 ± 0.43 | 11.71 ± 0.78 | |
NBA | 8.81 ± 1.17 ab | 8.75 ± 0.55 a | 9.91 ± 0.46 b | 9.85 ± 0.47 b | 10.95 ± 0.77 b | |
NWA | 7.81 ± 1.11 ab | 8.34 ± 0.55 a | 9.51 ± 0.43 bc | 9.30 ± 0.47 bc | 10.52 ± 0.72 c | |
MBW | 1.65 ± 0.07 | 1.61 ± 0.05 | 1.63 ± 0.04 | 1.66 ± 0.05 | 1.58 ± 0.07 | |
MWW | 6.21 ± 0.11 a | 6.64 ± 0.04 b | 6.65 ± 0.03 b | 6.63 ± 0.03 b | 6.65 ± 0.05 b |
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Norseeda, W.; Liu, G.; Teltathum, T.; Supakankul, P.; Sringarm, K.; Naraballobh, W.; Khamlor, T.; Chomdej, S.; Nganvongpanit, K.; Krutmuang, P.; et al. Association of IL-4 and IL-4R Polymorphisms with Litter Size Traits in Pigs. Animals 2021, 11, 1154. https://doi.org/10.3390/ani11041154
Norseeda W, Liu G, Teltathum T, Supakankul P, Sringarm K, Naraballobh W, Khamlor T, Chomdej S, Nganvongpanit K, Krutmuang P, et al. Association of IL-4 and IL-4R Polymorphisms with Litter Size Traits in Pigs. Animals. 2021; 11(4):1154. https://doi.org/10.3390/ani11041154
Chicago/Turabian StyleNorseeda, Worrarak, Guisheng Liu, Tawatchai Teltathum, Pantaporn Supakankul, Korawan Sringarm, Watcharapong Naraballobh, Trisadee Khamlor, Siriwadee Chomdej, Korakot Nganvongpanit, Patcharin Krutmuang, and et al. 2021. "Association of IL-4 and IL-4R Polymorphisms with Litter Size Traits in Pigs" Animals 11, no. 4: 1154. https://doi.org/10.3390/ani11041154