Induction of Spermiation in Sterlet Acipenser ruthenus by PLGA Microparticle Delivery with Sustained Alarelin Release
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Fish Rearing and Pre-Spawning Water Conditions
2.2. Treatment
2.2.1. Synthesis of PLGA Microparticles
2.2.2. Treatments
2.3. Sample Collection
2.3.1. Blood Collection
2.3.2. Milt Collection
2.4. Analysis of Samples
2.4.1. 11-Ketotestosterone and Testosterone Analysis
2.4.2. Sperm Quantitative Parameters
2.4.3. Sperm Qualitative Parameters
2.5. Statistical Analysis
2.5.1. Spermiation Rate
2.5.2. Quantitative Sperm Parameters and Androgen Concentrations
2.5.3. Sperm Qualitative Parameters
3. Results
3.1. Androgen Concentrations
3.1.1. Testosterone Concentrations
3.1.2. 11-KT Concentrations
3.2. Effect of Hormone Treatment on Sperm Production
3.2.1. Spermiation Rate
3.2.2. Sperm Quantitative Parameters
Milt Volume
Spermatozoon Concentration
Relative Sperm Production
3.2.3. Sperm Qualitative Parameters
Spermatozoon Curvilinear Velocity and Linearity
Spermatozoon Motility Rate
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Treatment | Fish Weight (kg) | Dose |
---|---|---|
0.9% NaCl | 1.57 ± 0.23 | 1 mL/kg |
Carp pituitary extract | 1.59 ± 0.22 | 4 mg/kg |
PLGA35 1 | 1.59 ± 0.25 | 35 µg/kg Alarelin |
PLGA200 1 | 1.58 ± 0.27 | 200 µg/kg Alarelin |
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Podhorec, P.; Knowles, J.; Vysloužil, J.; Boryshpolets, S.; Kubová, K.; Rodina, M.; Kholodnyy, V.; Sotnikov, A.; Gela, D.; Dzyuba, B. Induction of Spermiation in Sterlet Acipenser ruthenus by PLGA Microparticle Delivery with Sustained Alarelin Release. Animals 2021, 11, 3305. https://doi.org/10.3390/ani11113305
Podhorec P, Knowles J, Vysloužil J, Boryshpolets S, Kubová K, Rodina M, Kholodnyy V, Sotnikov A, Gela D, Dzyuba B. Induction of Spermiation in Sterlet Acipenser ruthenus by PLGA Microparticle Delivery with Sustained Alarelin Release. Animals. 2021; 11(11):3305. https://doi.org/10.3390/ani11113305
Chicago/Turabian StylePodhorec, Peter, Jindřiška Knowles, Jakub Vysloužil, Sergii Boryshpolets, Kateřina Kubová, Marek Rodina, Vitaliy Kholodnyy, Anatolii Sotnikov, David Gela, and Borys Dzyuba. 2021. "Induction of Spermiation in Sterlet Acipenser ruthenus by PLGA Microparticle Delivery with Sustained Alarelin Release" Animals 11, no. 11: 3305. https://doi.org/10.3390/ani11113305