miR-F4-C12 Functions on the Regulation of Adipose Accumulation by Targeting PIK3R1 in Castrated Male Pigs
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals and Sample Collection
2.2. Cell Differentiation and Transfection
2.3. Oil Red O Staining
2.4. RNA Preparation and qRT-PCR
2.5. Plasmid Construction
2.6. Dual-Luciferase Reporter Assay
2.7. Protein Isolation and Western Blot Analysis
2.8. Statistical Data Analysis
3. Result
3.1. Identification of miRNA Expression in Backfat Tissue
3.2. Expression Profiles of miRNAs during Adipogenic Differentiation in 3T3 Cells
3.3. miR-F4-C12 Promote the Differentiation of 3T3-L1 Cells
3.4. PIK3R1 Was the Direct Target of miR-F4-C12
3.5. PIK3R1 Expression in Backfat Tissue
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Acknowledgments
Conflicts of Interest
References
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Primer Name | Primer Sequence (5′-3′) | Application |
---|---|---|
miR-F4-C12 | RT: CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAACACTGA | qRT-PCR |
F: TCTCGCACGC TAGCACCATT | ||
R: CTCAACTGGTGTCGTGGAGTC | ||
miR-F3-C14 | RT: CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCGCCAATA | qRT-PCR |
F: GGG TAGCAGCACGTAAATATT | ||
R: CTCAACTGGTGTCGTGGAGTC | ||
miR-F4-S104 | RT: CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTTCAGTCA | qRT-PCR |
F: GGG CATCTGTGGGATTATGA | ||
R: CTCAACTGGTGTCGTGGAGTC | ||
miR-181a-2 | RT: CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAACTCACC | qRT-PCR |
F: TCTCGCACGC AACATTCAACGCTG | ||
R: CTCAACTGGTGTCGTGGAGTC | ||
miR-143 | RT: CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGGAGCTACA | qRT-PCR |
F: TCTCGCACGCTGAGATGAAGC | ||
R: CTCAACTGGTGTCGTGGAGTC | ||
PIK3R1 | F: GACTTGCCCCACCACGAT | qRT-PCR |
R: AGTGCTTGACCTCGCCGT | ||
PIK3R1-wt-3′ UTR | F: ATTGCGATCGCGCCACCTGCTCAAGTTCA | Luciferase reporter |
R: ATTGTTTAAACGTTCCCCAAAGTGTTCCTCT | ||
PIK3R1-mut- 3′ UTR | F: ATTGCGATCGCGCCACCTGCTCAAGTTCA | Luciferase reporter |
R: ATTGTTTAAACGCTTCTGAAAGCATGAACATCGTGGTTGGTGAAAGGCCTCTTTG |
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Xu, Q.; Chen, J.; Liu, X.; Luo, Y.; Wang, T.; Fang, M. miR-F4-C12 Functions on the Regulation of Adipose Accumulation by Targeting PIK3R1 in Castrated Male Pigs. Animals 2021, 11, 3053. https://doi.org/10.3390/ani11113053
Xu Q, Chen J, Liu X, Luo Y, Wang T, Fang M. miR-F4-C12 Functions on the Regulation of Adipose Accumulation by Targeting PIK3R1 in Castrated Male Pigs. Animals. 2021; 11(11):3053. https://doi.org/10.3390/ani11113053
Chicago/Turabian StyleXu, Qiao, Jie Chen, Ximing Liu, Yabiao Luo, Tianzuo Wang, and Meiying Fang. 2021. "miR-F4-C12 Functions on the Regulation of Adipose Accumulation by Targeting PIK3R1 in Castrated Male Pigs" Animals 11, no. 11: 3053. https://doi.org/10.3390/ani11113053