Immune-Related Gene Expression Profiling of Broiler Chickens Fed Diets Supplemented with Vinification Byproducts: A Valorization Approach II
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Broilers’ Trial
2.2. Vinification Byproducts Processing and Diets Formulation
2.3. Determination of Total Antioxidant Capacity of Vinification Byproducts
2.4. Determination of Total b-1,3-1,6-Glucans of Wine Lees
2.5. Determination of Performance Parameters
2.6. Sample Collection
2.7. Molecular Analysis
2.7.1. RNA Isolation and cDNA Synthesis
2.7.2. Primers’ Design
2.7.3. Real-Time Quantitative PCR
2.8. Statistics
3. Results
3.1. Grape Byproducts Total Antioxidant Capacity and Wine Lee b-Glucan Content
3.2. Broilers’ Performance
3.3. Relative Transcript Levels of Genes Regulating the Immune System in Liver
3.4. Relative Transcript Levels of Genes Regulating the Immune System in the Bursa of Fabricius
3.5. Relative Transcript Levels of Genes Regulating the Immune System in the Spleen
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Sequence | Amplicon bp | Accession No.* | References |
---|---|---|---|---|
GAPDH | F: 5′- GCTGGCATTGCACTGAATGAC -3′ | 113 | NM_204305.1 | [23] |
R: 5′- CACTCCTTGGATGCCATGT -3′ | ||||
ACTB | F: 5′- AGCGAACGCCCCCAAAGTTCT -3′ | 139 | NM_205518.1 | [24] |
F: 5′- AGCTGGGCTGTTGCCTTCACA -3′ | ||||
TLR4 | R: 5′- ACCCGAACTGCAGTTTCTGGAT -3′ | 120 | NM_001030693.1 | [23] |
R: 5′- AGGTGCTGGAGTGAATTGGC -3′ | ||||
MAPK | F: 5′- GAACGTGCGCTTCATCTACG -3′ | 137 | XM_040649449.1 | |
R: 5′- CCACGGGCTTAAACGCTTTC -3′ | ||||
NFKB | F: 5′- GAAGGAATCGTACCGGGAACA -3′ | 131 | NM_205134 131 | [25] |
R: 5′- CTCAGAGGGCCTTGTGACAGTAA -3′ | ||||
TNF | F: 5′- CCCCTACCCTGTCCCACAA -3′ | 67 | NM204267 | [26] |
R: 5′- TGAGTACTGCGGAGGGTTCAT -3′ | ||||
INFA | F: 5′- ACTTCAGCTGCCTCCACACCTT -3′ | 92 | AM049251.1 | [23] |
R: 5′- CAGGAACCAGGCACGAGCTT -3′ | ||||
INFG | F: 5′- AACAACCTTCCTGATGGCGTGA -3′ | 89 | NM_205149.1 | [23] |
R: 5′- GCTTTGCGCTGGATTCTCAAGT -3′ | ||||
IL1B | F: 5′- TGCTTCGTGCTGGAGTCACCC -3′ | 98 | XM_015297469.1 | [23] |
R: 5′- GGCCGGTACAGCGCAATGTT -3′ | ||||
IL2 | F: 5′- CGTAAGTGGATGGTTTTCCTCT -3′ | 161 | NM204153 | [27] |
R: 5′- GGCTAAAGCTCACCTGGGTC -3′ | ||||
IL6 | F: 5′- AGCGAAAAGCAGAACGTCGAGTC -3′ | 107 | XM_015281283.2 | [23] |
R: 5′- GCCGAGTCTGGGATGACCACTTC -3′ | ||||
IL8 | F: 5′- CTGGCCCTCCTCCTGGTT-3′ | 105 | HM179639 | [28] |
R: 5′- GCAGCTCATTCCCCATCTTTAC -3′ | ||||
IL18 | F: 5′- GTTGTTCGATTTAGGGAAGGAG -3′ | 146 | NM204608.1 | [29] |
R: 5′- TCAAAGGCCAAGAACATTCC -3′ |
Dietary Treatment | |||
---|---|---|---|
GGP | WYC | PE | |
FRAP | 0.95 ± 0.07 | 2.6 ± 0.1 | 2.1 ± 0.2 |
DPPH | 1.56 ± 0.01 | 1.846 ± 0.001 | 1.860 ± 0.007 |
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Mavrommatis, A.; Simitzis, P.E.; Kyriakaki, P.; Giamouri, E.; Myrtsi, E.D.; Evergetis, E.; Filippi, K.; Papapostolou, H.; Koulocheri, S.D.; Pappas, A.C.; et al. Immune-Related Gene Expression Profiling of Broiler Chickens Fed Diets Supplemented with Vinification Byproducts: A Valorization Approach II. Animals 2021, 11, 3038. https://doi.org/10.3390/ani11113038
Mavrommatis A, Simitzis PE, Kyriakaki P, Giamouri E, Myrtsi ED, Evergetis E, Filippi K, Papapostolou H, Koulocheri SD, Pappas AC, et al. Immune-Related Gene Expression Profiling of Broiler Chickens Fed Diets Supplemented with Vinification Byproducts: A Valorization Approach II. Animals. 2021; 11(11):3038. https://doi.org/10.3390/ani11113038
Chicago/Turabian StyleMavrommatis, Alexandros, Panagiotis E. Simitzis, Panagiota Kyriakaki, Elisavet Giamouri, Eleni D. Myrtsi, Epameinondas Evergetis, Katiana Filippi, Harris Papapostolou, Sofia D. Koulocheri, Athanasios C. Pappas, and et al. 2021. "Immune-Related Gene Expression Profiling of Broiler Chickens Fed Diets Supplemented with Vinification Byproducts: A Valorization Approach II" Animals 11, no. 11: 3038. https://doi.org/10.3390/ani11113038