Effects of Inositol Supplementation in Sperm Extender on the Quality of Cryopreserved Mesopotamian Catfish (Silurus triostegus, H. 1843) Sperm
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Study Area and Semen Collection
2.2. Chemicals
2.3. Sperm Evaluation
Sperm Motility, Duration, and Concentration
2.4. Cryopreservation Protocol
2.5. Preparation of Sperm Samples for Biochemical Analysis
2.5.1. Oxidative Stress Analysis
2.5.2. Measurement of Oxidative Stress Status
2.6. DNA Damage Measurement
2.6.1. Measurement of 8-Hydroxydeoxyguanosine (8-OHdG) in Fish Sperm
2.6.2. Comet Assay
2.7. Apoptotic Analysis by ELISA of Fish Sperm Cells
2.8. Statistics
3. Results
3.1. Evaluation of Sperm Parameters, Oxidant and Antioxidant Status, DNA Damage
3.1.1. Effect of Inositol on Post-Thaw Sperm Motility and Duration of Motility
3.1.2. Effect of Inositol on Post-Thaw Antioxidant and Oxidant Status of Sperm
3.1.3. Changes in DNA Damage Parameters and Apoptosis in Post-Thaw Sperm with Frozen Glucose Diluent Supplemented with Inositol
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Dose of Inositol (Groups) | Parameters | ||
---|---|---|---|
Motility Rate (%) | Motility Duration (s) | ||
Control | 50.00 ± 2.58 a | 55.00 ± 3.41 a | |
5 mg | 35.00 ± 2.14 b | 34.00 ± 2.49 b | |
10 mg | 29.00 ± 2.33 b | 29.8 ± 03.25 b | |
20 mg | 47.00 ± 2.13 a | 52.00 ± 3.55 a | |
40 mg | 51.00 ± 1.79 a | 56.80 ± 1.40 a | |
Pr > F 1 | ANOVA | 0.00 | 0.00 |
Linear trend | 0.01 | 0.01 | |
Quatratic trend | 0.32 | 0.03 |
Dose of Inositol (Groups) | Parameters | |||||
---|---|---|---|---|---|---|
TAS (mmol Trolox Equiv/L) | TOS (μmol H2O2 Equiv/L) | OSI (AU) | GSH (nmol/g) | MDA (nmol/g) | ||
Control | 1.11 ± 0.04 c | 16.03 ± 0.43 a | 1.24 ± 0.01 a | 27.53 ± 0.22 c | 22.17 ± 0.54 a | |
5 mg | 1.24 ± 0.01 bc | 15.37 ± 0.01 a | 1.28 ± 0.03 a | 30.67 ± 0.35 c | 18.45 ± 0.30 b | |
10 mg | 1.32 ± 0.03 b | 12.11 ± 0.25 b | 0.86 ± 0.07 b | 34.29 ± 0.13 b | 17.21 ± 0.31 b | |
20 mg | 1.80 ± 0.08 a | 9.47 ± 0.81 c | 0.52 ± 0.05 c | 37.84 ± 0.51 a | 8.54 ± 0.22 c | |
40 mg | 1.86 ± 0.02 a | 8.90 ± 0.81 c | 0.47 ± 0.01 c | 37.89 ± 0.37 a | 7.78 ± 0.17 c | |
Pr > F 1 | ANOVA | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
Linear trend | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |
Quatratic trend | 0.67 | 0.15 | 0.00 | 0.02 | 0.41 |
Dose of Inositol (Groups) | Parameters | ||
---|---|---|---|
8-OHdG (ng/mL) | Comet (AU) | ||
Control | 140.00 ± 1.00 a | 37.52 ± 1.27 a | |
5 mg | 110.15 ± 0.21 c | 28.74 ± 0.47 b | |
10 mg | 115.07 ± 1.03 c | 24.03 ± 0.39 c | |
20 mg | 125.00 ± 1.00 b | 22.65 ± 0.21 c | |
40 mg | 73.01 ± 0.30 d | 10.55 ± 0.19 d | |
Pr > F 1 | ANOVA | 0.00 | 0.00 |
Linear trend | 0.01 | 0.00 | |
Quatratic trend | 0.00 | 0.00 |
Dose of Inositol (Groups) | Parameters | |
---|---|---|
Apoptozis (AR %) | ||
Control | 2.45 ± 0.04 a | |
5 mg | 1.98 ± 0.03 b | |
10 mg | 1.84 ± 0.03 c | |
20 mg | 1.31 ± 0.03 d | |
40 mg | 1.22 ± 0.02 d | |
Pr > F 1 | ANOVA | 0.00 |
Linear trend | 0.00 | |
Quatratic trend | 0.00 |
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Doğu, Z.; Şahinöz, E.; Aral, F.; Koyuncu, İ.; Yüksekdağ, Ö. Effects of Inositol Supplementation in Sperm Extender on the Quality of Cryopreserved Mesopotamian Catfish (Silurus triostegus, H. 1843) Sperm. Animals 2021, 11, 3029. https://doi.org/10.3390/ani11113029
Doğu Z, Şahinöz E, Aral F, Koyuncu İ, Yüksekdağ Ö. Effects of Inositol Supplementation in Sperm Extender on the Quality of Cryopreserved Mesopotamian Catfish (Silurus triostegus, H. 1843) Sperm. Animals. 2021; 11(11):3029. https://doi.org/10.3390/ani11113029
Chicago/Turabian StyleDoğu, Zafer, Erdinç Şahinöz, Faruk Aral, İsmail Koyuncu, and Özgür Yüksekdağ. 2021. "Effects of Inositol Supplementation in Sperm Extender on the Quality of Cryopreserved Mesopotamian Catfish (Silurus triostegus, H. 1843) Sperm" Animals 11, no. 11: 3029. https://doi.org/10.3390/ani11113029