Litopenaeus vannamei BMAL1 Is a Critical Mediator Regulating the Expression of Glucose Transporters and Can Be Suppressed by Constant Darkness
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Gene Expression (BMAL1, GLUT1 and SGLT1) Daily over 24-h in the Intestine and Hepatopancreas
2.2. Protein Sequence and Domain Structure Analysis
2.3. In Vitro Dual-Luciferase Reporter Assays
2.4. In Vivo Knockdown by dsRNA Injection
2.5. Eight-Week Light/Dark Treatment
2.6. Quantitative Reverse Transcription PCR (RT-qPCR)
2.7. Statistical Analysis
3. Results
3.1. Daily Profile of BMAL1, GLUT1 and SGLT1 Expression in the Hepatopancreas and Intestine
3.2. The Sequence Characteristics of BMAL1
3.3. Overexpression of BMAL1 Significantly Activated the Gene Expression of GLUT1 and SGLT1 In Vitro
3.4. Knockdown of BMAL1 Decreased the mRNA Expression of Glucose Transporters (GLUT1, SGLT1) in the Hepatopancreas and Intestine of L. vannamei
3.5. Effects of Dark Treatment on Growth Performance and Survival Rate in L. vannamei
3.6. Effects of Dark Treatment on Serum Glucose Concentration in L. vannamei
3.7. Effects of Dark Treatment on the mRNA Expression of BMAL1, GLUT1 and SGLT1 in the Hepatopancreas and Intestine of L. vannamei
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Primers | Sequences (5’ to 3’) |
---|---|
For RNA interference experiment | |
GFP-F | ATGGTGAGCAAGGGCGAGGAG |
GFP-R | TTACTTGTACAGCTCGTCCATGCC |
T7-GFP-F | GGATCCTAATACGACTCACTATAGGATGGTGAGCAAGGGCGAGGAG |
T7-GFP-R | GGATCCTAATACGACTCACTATAGGTTACTTGTACAGCTCGTCCATGCC |
BMAL1-F | ATGTTTGATCTTGGCAACTATG |
BMAL1-R | AAAGTTTGATACACCGATTCCG |
T7-BMAL1-F | GGATCCTAATACGACTCACTATAGGATGTTTGATCTTGGCAACTATG |
T7-BMAL1-R | GGATCCTAATACGACTCACTATAGGAAAGTTTGATACACCGATTCCG |
For RT-PCR | |
BMAL1-F | CCTTCAACCACAATAACAACCATAC |
BMAL1-R | CCTCGTCTGAGTCGCTCGTGCCATC |
GLUT1-F | CTTGGAGTTGGGTCGGTGATGGTTA |
GLUT1-R | TCCACGGAATACTGCCAGGACCCAC |
SGLT1-F | ATCGGCTTGGTCATAGGACTCATTC |
SGLT1-R | GCAGCCGAAGTGGAGATAATGGACA |
β-actin-F | CCACGAGACCACCTACAAC |
β-actin-R | AGCGAGGGCAGTGATTTC |
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Jiao, L.; Dai, T.; Sun, P.; Jin, M.; Zhou, Q. Litopenaeus vannamei BMAL1 Is a Critical Mediator Regulating the Expression of Glucose Transporters and Can Be Suppressed by Constant Darkness. Animals 2021, 11, 2893. https://doi.org/10.3390/ani11102893
Jiao L, Dai T, Sun P, Jin M, Zhou Q. Litopenaeus vannamei BMAL1 Is a Critical Mediator Regulating the Expression of Glucose Transporters and Can Be Suppressed by Constant Darkness. Animals. 2021; 11(10):2893. https://doi.org/10.3390/ani11102893
Chicago/Turabian StyleJiao, Lefei, Tianmeng Dai, Peng Sun, Min Jin, and Qicun Zhou. 2021. "Litopenaeus vannamei BMAL1 Is a Critical Mediator Regulating the Expression of Glucose Transporters and Can Be Suppressed by Constant Darkness" Animals 11, no. 10: 2893. https://doi.org/10.3390/ani11102893