Vitrification of Donkey Sperm: Is It Better Using Permeable Cryoprotectants?
Veterinary Reproduction Group, Department of Medicine and Animal Surgery, Faculty of Veterinary Medicine, University of Cordoba, 14071 Cordoba, Spain
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Received: 30 July 2020 / Revised: 13 August 2020 / Accepted: 17 August 2020 / Published: 20 August 2020
Conventional donkey sperm-freezing using permeable cryoprotectants has been successfully performed, and good sperm parameters have been obtained after thawing. Unfortunately, artificial insemination of jennies with cryopreserved semen has given unsatisfactory results. Vitrification by directly dropping the sperm into the liquid nitrogen following the spheres methodology has been developed in human beings as an alternative to conventional freezing. This technique has shown to be a species-specific methodology and the concentration of cryoprotectants should be optimized in donkeys. Additionally, in this study, a permeable cryoprotectant (glycerol) has been tested for the first time for donkey sperm vitrification. According to our findings, vitrification of donkey sperm was effectively carried out using an extender supplemented with sucrose or bovine serum albumin (BSA) as non-permeable agent. When glycerol, a permeable agent, was compared to sucrose 0.1 M and BSA 5%, sperm quality significantly decreased. Therefore, donkey sperm vitrification in the absence of permeable agents obtained better results and gives a new approach to create a pattern for future studies of fertility trials.