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Article

Mobility and Invasion Related Gene Expression Patterns in Equine Sarcoid

1
Department of Animal Molecular Biology, Laboratory of Genomics, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland
2
Department of Animal Reproduction, Anatomy and Genomics, University of Agriculture in Kraków, Mickiewicza24/28, 30-059 Kraków, Poland
3
University Centre of Veterinary Medicine, University of Agriculture in Kraków, Mickiewicza 24/28, 30-059 Kraków, Poland
*
Author to whom correspondence should be addressed.
Animals 2020, 10(5), 880; https://doi.org/10.3390/ani10050880
Received: 2 March 2020 / Revised: 27 April 2020 / Accepted: 14 May 2020 / Published: 19 May 2020
(This article belongs to the Special Issue Horse Breeding and Genetics)
The current studies profiled the expression of five equine sarcoid cell genes related to cell mobility and invasion (cell cycle control binding protein alpha, coronin 1b, metalloproteinase 2, tissue inhibitor of metalloproteinases 3 and vimentin) and compared the expression of these genes in healthy skin fibroblasts. Cells were collected from healthy and sarcoid-affected skin biopsies obtained by a qualified veterinarian. Gene expression patterns were investigated under two different conditions of cell culture, with high and low availability of nutritional components in the culture medium. The results showed significant differences in the expression of the two analyzed genes (coronin 1b and vimentin) depending on culture conditions. The obtained results emphasize the complexity of the genomic background of sarcoids and indicate the importance of further research on genes related to the physiological changes that occur in sarcoids.
Sarcoids are the most common skin neoplasm in the Equidae family. Sarcoids are benign, but may cause severe damage in affected animals. Due to the high risk of post-treatment recurrence and the lack of an effective method of treatment, it is reasonable to perform studies on the molecular aspects of this neoplasm. Therefore, the present studies analyzed five genes (cell cycle control binding protein alpha, coronin 1b, metalloproteinase 2, tissue inhibitor of metalloproteinases 3 and vimentin) related to cell mobility and invasion traits. Primary healthy fibroblasts and sarcoid cells were obtained from skin biopsies. Cell lines were cultured in two different medium types with different concentrations of foetal bovine serum (10% and 0.5% FBS) to study its influence on the analyzed genes. Gene expression was measured using the real-time PCR method. The results showed significant differences in two genes (coronin and vimentin) depending on culture conditions. In conclusion, the results enabled finding two new genes, related to cell motility and invasion traits, in which gene expression is deregulated. Results of the study may put new knowledge into the complexity of the genetic background of this disease and show the importance of further analysis on this subject. View Full-Text
Keywords: skin neoplasia; horse; cell physiology; real-time PCR skin neoplasia; horse; cell physiology; real-time PCR
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MDPI and ACS Style

Podstawski, P.; Witarski, W.; Szmatoła, T.; Bugno-Poniewierska, M.; Ropka-Molik, K. Mobility and Invasion Related Gene Expression Patterns in Equine Sarcoid. Animals 2020, 10, 880. https://doi.org/10.3390/ani10050880

AMA Style

Podstawski P, Witarski W, Szmatoła T, Bugno-Poniewierska M, Ropka-Molik K. Mobility and Invasion Related Gene Expression Patterns in Equine Sarcoid. Animals. 2020; 10(5):880. https://doi.org/10.3390/ani10050880

Chicago/Turabian Style

Podstawski, Przemysław, Wojciech Witarski, Tomasz Szmatoła, Monika Bugno-Poniewierska, and Katarzyna Ropka-Molik. 2020. "Mobility and Invasion Related Gene Expression Patterns in Equine Sarcoid" Animals 10, no. 5: 880. https://doi.org/10.3390/ani10050880

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