Mobility and Invasion Related Gene Expression Patterns in Equine Sarcoid
Department of Animal Molecular Biology, Laboratory of Genomics, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland
Department of Animal Reproduction, Anatomy and Genomics, University of Agriculture in Kraków, Mickiewicza24/28, 30-059 Kraków, Poland
University Centre of Veterinary Medicine, University of Agriculture in Kraków, Mickiewicza 24/28, 30-059 Kraków, Poland
Author to whom correspondence should be addressed.
Received: 2 March 2020 / Revised: 27 April 2020 / Accepted: 14 May 2020 / Published: 19 May 2020
The current studies profiled the expression of five equine sarcoid cell genes related to cell mobility and invasion (cell cycle control binding protein alpha, coronin 1b, metalloproteinase 2, tissue inhibitor of metalloproteinases 3 and vimentin) and compared the expression of these genes in healthy skin fibroblasts. Cells were collected from healthy and sarcoid-affected skin biopsies obtained by a qualified veterinarian. Gene expression patterns were investigated under two different conditions of cell culture, with high and low availability of nutritional components in the culture medium. The results showed significant differences in the expression of the two analyzed genes (coronin 1b and vimentin) depending on culture conditions. The obtained results emphasize the complexity of the genomic background of sarcoids and indicate the importance of further research on genes related to the physiological changes that occur in sarcoids.