Semen Parameters of Fertile Guinea Pigs (Cavia porcellus) Collected by Transrectal Electroejaculation
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animal Selection
2.2. General and Andrological Examination
2.3. Animal Handling
2.4. Semen Collection by Electroejaculation
2.4.1. The Electroejaculator Device
2.4.2. Sedation Protocol and Electroejaculation
2.4.3. Semen Collection
2.5. Semen Evaluation
2.5.1. Macroscopic Evaluation
2.5.2. Microscopic Evaluation
2.5.3. Sperm Count and Concentration
2.5.4. Morphology
2.5.5. Sperm Ubiquitin Assay
2.6. Data Analysis
3. Results
3.1. Semen Collections
3.2. Seminal Characteristics
4. Discussion
4.1. Electroejaculation Efficiency
4.2. Macroscopic Parameter Values and Variability:
4.3. Microscopic Parameters and their Variability
4.4. Semen Quality at Successive Electroejaculations
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Parameter | n | Minimum | Maximum | Mean ± SD |
---|---|---|---|---|
Volume (mL) | 39 | 0.10 | 2.40 | 0.67 ± 0.5 |
Motility (%) | 39 | 63.00 | 99.00 | 91 ± 6.6 |
Concentration (×106 spermatozoa/mL) | 37 | 6.00 | 94.60 | 36.70 ± 28.4 |
Total number of spermatozoa (×106 spermatozoa/ejaculate) | 37 | 1.40 | 60.52 | 20.09 ± 17.6 |
Acrosomal defect (%) | 39 | 0.63 | 21.24 | 7.80 ± 5.2 |
Acrosomal absence (%) | 39 | 0.44 | 25.08 | 6.51 ± 6.3 |
Other abnormalities (%) | 39 | 0.25 | 23.32 | 3.95 ± 4.5 |
Total abnormalities (%) | 39 | 3.22 | 33.95 | 18.26 ± 8.5 |
Highly ubiquitinated spermatozoa (%) | 37 | 0.35 | 31.21 | 5.57 ± 6.3 |
Ph | 14 | 7.00 | 8.0 | 7.04 ± 0.1 |
Parameter | Ejaculate 2 | Ejaculate 3 | Ejaculate 4 | |||
---|---|---|---|---|---|---|
n | Mean ± SD | n | Mean ± SD | n | Mean ± SD | |
Volume (mL) | 16 | 0.5 a ± 0.5 | 13 | 0.6 a ± 0.4 | 10 | 0.9 a ± 0.8 |
Motility (%) | 16 | 92 a ± 6.0 | 13 | 88 a ± 8.8 | 10 | 93 a ± 2.9 |
Concentration (×106 spermatozoa/mL) | 14 | 34.6 a ± 27.5 | 13 | 40.7 a ± 31.8 | 10 | 34.3 a ± 27.4 |
Total number of spermatozoa (×106 spermatozoa/ejaculate) | 14 | 2.6 a ± 11.3 | 13 | 23.3 a ± 18.5 | 10 | 26.2 a ± 21.2 |
Total abnormalities (%) | 16 | 16.7 a ± 7.7 | 13 | 18.3 a ± 9 | 10 | 20.5 a ± 9.3 |
Ubiquitinated spermatozoa (%) | 14 | 4.8 a ± 7.8 | 13 | 6.1 a ± 5.9 | 10 | 5.9 a ± 4.7 |
Characteristics | Ejaculate 2 | Ejaculate 3 | Ejaculate 4 | |||
---|---|---|---|---|---|---|
n | Mean ± SD | n | Mean ± SD | n | Mean ± SD | |
Abnormal acrosome (%) | 16 | 6.98 a ± 4.31 | 13 | 7.99 a ± 4.66 | 10 | 8.86 a ± 7.12 |
Acrosomal absence (%) | 16 | 5.17 a ± 5.91 | 13 | 7.03 a ± 6.05 | 10 | 7.98 a ± 7.22 |
Other abnormalities (%) | 16 | 4.60 a ± 5.55 | 13 | 3.35 a ± 3.61 | 10 | 3.70 a ± 4.05 |
Total abnormalities (%) | 16 | 16.75 a ± 7.79 | 13 | 18.37 a ± 9.04 | 10 | 20.53 a ± 9.31 |
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Benavides, F.; Sutovsky, P.; López, V.; Kennedy, C.; Echevarría, L. Semen Parameters of Fertile Guinea Pigs (Cavia porcellus) Collected by Transrectal Electroejaculation. Animals 2020, 10, 767. https://doi.org/10.3390/ani10050767
Benavides F, Sutovsky P, López V, Kennedy C, Echevarría L. Semen Parameters of Fertile Guinea Pigs (Cavia porcellus) Collected by Transrectal Electroejaculation. Animals. 2020; 10(5):767. https://doi.org/10.3390/ani10050767
Chicago/Turabian StyleBenavides, Fernando, Peter Sutovsky, Verónica López, Chelsey Kennedy, and Luisa Echevarría. 2020. "Semen Parameters of Fertile Guinea Pigs (Cavia porcellus) Collected by Transrectal Electroejaculation" Animals 10, no. 5: 767. https://doi.org/10.3390/ani10050767
APA StyleBenavides, F., Sutovsky, P., López, V., Kennedy, C., & Echevarría, L. (2020). Semen Parameters of Fertile Guinea Pigs (Cavia porcellus) Collected by Transrectal Electroejaculation. Animals, 10(5), 767. https://doi.org/10.3390/ani10050767