The Association of the Copy Number Variation of the MLLT10 Gene with Growth Traits of Chinese Cattle
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animal Selection and Measurement of Body Size Data
2.2. Sample Collected, Genomic DNA Extraction
2.3. Primer Design and Amplification Detection
2.4. Detection of the MLLT10 Gene Copy Number
2.5. Data Analysis Processing
3. Results
3.1. Expression Profile of the MLLT10 Gene
3.2. Distribution of the MLLT10 Gene Copy Number in the Experimental Sample Group
3.3. Correlation Analysis in the MLLT10 Gene CNVs and Growth Traits of Different Cattle Breeds
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Primer | Sequence (5′–3′) | Product Length | |
---|---|---|---|
MLLT10-CNV | Forward primer | TCCAAGGACAAGAACCCTGC | 114 bp |
Reverse primer | GCTCCTCTTAGGCCCTTGTC | ||
BTF3 | Forward primer | AACCAGGAGAAACTCGCCAA | 166 bp |
Reverse primer | TTCGGTGAAATGCCCTCTCG | ||
MLLT10 (mRNA) | Forward primer | GGAAGTCTCTGCGCACACTA | 175 bp |
Reverse primer | TGAAAAACTGCCTTGCTGGA | ||
β-actin (mRNA) | Forward primer | GTCATCACCATCGGCAATGAG | 84 bp |
Reverse primer | AATGCCGCAGGATTCCATG |
Chinese Yellow Cattle Breeds | Growth Traits | CNV Types (Average Value ± Standard Error) | p Value | ||
---|---|---|---|---|---|
Deletion (n = 26) | Normal (n = 12) | Duplication (n = 56) | |||
YL | Rump Length (cm) | 51.73 ± 4.285 a | 52.67 ± 3.367 a | 49.98 ± 3.952 b | 0.045 * |
Hucklebone Width (cm) | 22.85 ± 2.572 a | 20.58 ± 2.712 b | 22.79 ± 2.708 a | 0.031 * | |
XN | Deletion (n = 70) | Normal (n = 22) | Duplication (n = 13) | ||
Cannon Bone Circumference (cm) | 19.19 ± 1.477 a | 19.23 ± 1.343 a | 20.38 ± 1.261 b | 0.022 * |
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Yang, P.; Zhang, Z.; Xu, J.; Qu, K.; Lyv, S.; Wang, X.; Cai, C.; Li, Z.; Wang, E.; Xie, J.; et al. The Association of the Copy Number Variation of the MLLT10 Gene with Growth Traits of Chinese Cattle. Animals 2020, 10, 250. https://doi.org/10.3390/ani10020250
Yang P, Zhang Z, Xu J, Qu K, Lyv S, Wang X, Cai C, Li Z, Wang E, Xie J, et al. The Association of the Copy Number Variation of the MLLT10 Gene with Growth Traits of Chinese Cattle. Animals. 2020; 10(2):250. https://doi.org/10.3390/ani10020250
Chicago/Turabian StyleYang, Peng, Zijing Zhang, Jiawei Xu, Kaixing Qu, Shijie Lyv, Xianwei Wang, Cuicui Cai, Zhiming Li, Eryao Wang, Jianliang Xie, and et al. 2020. "The Association of the Copy Number Variation of the MLLT10 Gene with Growth Traits of Chinese Cattle" Animals 10, no. 2: 250. https://doi.org/10.3390/ani10020250
APA StyleYang, P., Zhang, Z., Xu, J., Qu, K., Lyv, S., Wang, X., Cai, C., Li, Z., Wang, E., Xie, J., Ru, B., Xu, Z., Lei, C., Chen, H., Huang, B., & Huang, Y. (2020). The Association of the Copy Number Variation of the MLLT10 Gene with Growth Traits of Chinese Cattle. Animals, 10(2), 250. https://doi.org/10.3390/ani10020250