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Open AccessArticle

Variation in the Lipin 1 Gene Is Associated with Birth Weight and Selected Carcass Traits in New Zealand Romney Sheep

by Guan Wang 1,2, Huitong Zhou 1,2,3, Hua Gong 2,3, Jianning He 4, Yuzhu Luo 1,2,*, Jon G. H. Hickford 2,3,*, Jiang Hu 1,2, Jiqing Wang 1,2, Xiu Liu 1,2 and Shaobin Li 1,2
1
Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China
2
International Wool Research Institute, Gansu Agricultural University, Lanzhou 730070, China
3
Gene-Marker Laboratory, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand
4
College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China
*
Authors to whom correspondence should be addressed.
Animals 2020, 10(2), 237; https://doi.org/10.3390/ani10020237
Received: 28 November 2019 / Revised: 23 January 2020 / Accepted: 30 January 2020 / Published: 3 February 2020
Lipin 1 plays an important role in lipid metabolism. It not only acts as a phosphatidate phosphatase and directly participates in the synthesis of glycerol and osteolipid, but also acts as a transcriptional co-activator to indirectly regulate the expression of genes related to lipid metabolism. In livestock species, variation in the lipin 1 gene (LPIN1) has been reported in pigs, chickens, and dairy cows, but has not been investigated in sheep, and little is known about whether it might affect production traits in this globally important meat-producing species. In this study, we used polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analyses to search for variation in ovine LPIN1, and its effects on production and carcass traits were investigated in New Zealand Romney sheep. The results suggest that ovine LPIN1 is variable and it may have value as a genetic marker for improving meat production and carcass traits.
Lipin 1 plays an important role in lipid metabolism. In this study; we searched for variation in the ovine lipin 1 gene (LPIN1) in three gene regions (a 5′ non-coding region; a region containing an alternatively spliced exon in intron 4; and a region containing coding exon 6) using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis. The greatest amount of alleles was found in coding exon 6; with five sequences being detected. The effect of variation in this exon was investigated in 242 New Zealand Romney lambs derived from 12 sire-lines. The presence of variant E3 was associated with a decrease in birth weight (p = 0.005) and the proportion of leg yield (p = 0.045), but with an increase in hot carcass weight (p = 0.032) and the proportion of loin yield (p = 0.014). The presence of variant B3 was associated with an increased pre-weaning growth rate (p = 0.041), whereas the presence of variant C3 was associated with an increase in shoulder yield (p < 0.001). These results suggest that ovine LPIN1 variation may have value as a genetic marker for improving meat production and carcass traits. View Full-Text
Keywords: Lipin 1 gene (LPIN1); nucleotide variation; PCR-SSCP; birth weight; meat yield Lipin 1 gene (LPIN1); nucleotide variation; PCR-SSCP; birth weight; meat yield
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Wang, G.; Zhou, H.; Gong, H.; He, J.; Luo, Y.; Hickford, J.G.H.; Hu, J.; Wang, J.; Liu, X.; Li, S. Variation in the Lipin 1 Gene Is Associated with Birth Weight and Selected Carcass Traits in New Zealand Romney Sheep. Animals 2020, 10, 237.

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