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Open AccessArticle

Morphometric Evaluation of Spermatogenic Cells and Seminiferous Tubules and Exploration of Luteinizing Hormone Beta Polypeptide in Testis of Datong Yak

1
Key Laboratory of Yak Breeding Engineering, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Science, Lanzhou 730050, China
2
Department of Animal Reproduction Shaheed Benazir Bhutto, University of Veterinary and Animal Sciences, Sakrand 67210, Pakistan
3
State Key Laboratory of Grassland Agro Ecosystems, School of Life Sciences, Lanzhou University, Lanzhou 730050, China
*
Author to whom correspondence should be addressed.
Animals 2020, 10(1), 66; https://doi.org/10.3390/ani10010066
Received: 6 November 2019 / Revised: 23 December 2019 / Accepted: 28 December 2019 / Published: 30 December 2019
(This article belongs to the Section Animal Reproduction)
Previous studies revealed that luteinizing hormone βeta polypeptide (LHB) plays an essential role in fertilization. Therefore, we aimed to confirm the importance of LHB in the testis of yak and to determine their association with male yak fertility. Histomorphological analysis of the testes is essential for predicting the fertilizing ability of the bull. To the best our knowledge, this is the first study to evaluate the micro anatomical changes and histometric alternation in testes of Datong yak. These findings could help to predict the sperm production capacity and to understand the specific molecular mechanisms of LHB during spermatogenesis.
Histological examination of testes is essential for understanding infertility, sex development, and growth. Therefore, to understand the histomorphology of testes at different developmental stages, we performed hematoxylin and eosin staining of Yak testis. Our results revealed that the diameters of spermatogenic cells and their nuclei were significantly larger (p < 0.05) in the testis at six years compared to at six and 18 months. No significant difference was noted between 30 months and six years. The study was designed to compare the expression profile of LHB in Datong yak. The expression pattern of LHB was explored using quantitative PCR, semi-quantitative PCR, molecular bioinformatic, and Western blot analysis. Our observations indicated that expression of LHB was significantly higher (p < 0.05) in the testis of Datong yak. Western blotting indicated that the molecular mass of LHB protein was 16 kDa in yak. The protein encoded by yak LHB included conserved cysteine-knot domain regions. The high expression of LHB in testis indicated that LHB may be vital for the development of male gonads and the fertility of Datong yak. View Full-Text
Keywords: histomorphology; gene expression; LHB; yak; testis histomorphology; gene expression; LHB; yak; testis
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Kalwar, Q.; Chu, M.; Ahmad, A.A.; Ding, X.; Wu, X.; Bao, P.; Yan, P. Morphometric Evaluation of Spermatogenic Cells and Seminiferous Tubules and Exploration of Luteinizing Hormone Beta Polypeptide in Testis of Datong Yak. Animals 2020, 10, 66.

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