The Only Chemoreceptor Encoded by che Operon Affects the Chemotactic Response of Agrobacterium to Various Chemoeffectors
Abstract
:1. Introduction
2. Materials and Methods
2.1. Primers, Plasmids, Bacterial Strains and Growth Conditions
2.2. DNA Manipulations
2.3. Mutagenesis and Complementation of Atu0514
2.4. Chemotaxis Assays
2.5. Bacterial Two-Hybrid Analyses
2.6. Fluorescence Microscopy
2.7. Statistical Analysis
3. Results
3.1. MCP514 Is a Cytoplasmic Chemoreceptor, but Localized at Cell Poles
3.2. MCP514 Significantly Affects the Chemotactic Response of Agrobacterium Fabrum
3.3. Both CheW1 and CheW2 Interact with MCP514 but Do Not Affect the Cellular Localization of MCP514
3.4. Helical Structure of Hairpin Subdomain Is Required for the Cellular Localization of MCP514
3.5. Two Key Residues of Hairpin Subdomain Play a Key Role in Maintaining the Chemotactic Function of MCP514
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ye, J.; Gao, M.; Zhou, Q.; Wang, H.; Xu, N.; Guo, M. The Only Chemoreceptor Encoded by che Operon Affects the Chemotactic Response of Agrobacterium to Various Chemoeffectors. Microorganisms 2021, 9, 1923. https://doi.org/10.3390/microorganisms9091923
Ye J, Gao M, Zhou Q, Wang H, Xu N, Guo M. The Only Chemoreceptor Encoded by che Operon Affects the Chemotactic Response of Agrobacterium to Various Chemoeffectors. Microorganisms. 2021; 9(9):1923. https://doi.org/10.3390/microorganisms9091923
Chicago/Turabian StyleYe, Jingyang, Miaomiao Gao, Qingxuan Zhou, Hao Wang, Nan Xu, and Minliang Guo. 2021. "The Only Chemoreceptor Encoded by che Operon Affects the Chemotactic Response of Agrobacterium to Various Chemoeffectors" Microorganisms 9, no. 9: 1923. https://doi.org/10.3390/microorganisms9091923