Bacillus pumilus and Bacillus subtilis Promote Early Maturation of Cecal Microbiota in Broiler Chickens
Abstract
:1. Introduction
2. Materials and Methods
2.1. Birds, Diet and Experimental Design
2.2. Sample Collection and DNA Extraction
2.3. Sequencing and Data Analysis of Cecal Microbial Community
3. Results
3.1. Sequencing Data
3.2. Probiotics Improve the Cecal Microbial Alpha Diversity in Young Chickens
3.3. Probiotics Affect the Cecal Microbial Beta Diversity in Young Chickens
3.4. Probiotics Change Composition of Cecal Microbiota in Broiler Chickens
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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a. Effects of dietary treatments on microbial richness (observed operational taxonomic units). | ||||||||
Groups/Time | Mean Day-7 | SEM | Mean Day-14 | SEM | Mean Day-28 | SEM | Mean Day-42 | SEM |
NC | 110 3 | 11.9 | 100 b,3 | 13.3 | 188 2 | 11.9 | 241 1 | 13.3 |
PC | 92 2 | 11.9 | 109 b,2 | 13.3 | 188 1 | 11.9 | 202 1 | 13.3 |
BPL | 116 3 | 13.3 | 172 a,2 | 13.3 | 231 1 | 13.3 | 228 1 | 13.3 |
BPH | 118 2 | 11.9 | 163 a,1,2 | 11.9 | 202 1 | 11.9 | 198 1 | 13.3 |
BSL | 135 2 | 11.9 | 172 a,2 | 13.3 | 234 1 | 11.9 | 249 1 | 11.9 |
BSH | 106 2 | 11.9 | 177 a,1 | 13.3 | 221 1 | 11.9 | 199 1 | 13.3 |
b. Effects of dietary treatments on microbial evenness (Pielou index) at days 7, 14, 28, and 42. | ||||||||
Groups/Time | Mean Day-7 | SEM | Mean Day-14 | SEM | Mean Day-28 | SEM | Mean Day-42 | SEM |
NC | 0.77 ab,1 | 0.025 | 0.66 ab,2 | 0.029 | 0.75 ab,1,2 | 0.025 | 0.69 1,2 | 0.029 |
PC | 0.65 c,2 | 0.025 | 0.63 bc,2 | 0.029 | 0.77 a,1 | 0.025 | 0.61 2 | 0.029 |
BPL | 0.77 ab,1 | 0.029 | 0.52 c,2 | 0.029 | 0.74 ab,1 | 0.029 | 0.73 1 | 0.029 |
BPH | 0.68 bc,1,2 | 0.025 | 0.77 a,1 | 0.025 | 0.68 ab,2 | 0.025 | 0.64 2 | 0.029 |
BSL | 0.76 ab,1 | 0.025 | 0.68 ab,1,2 | 0.029 | 0.71 ab,12 | 0.025 | 0.63 2 | 0.025 |
BSH | 0.80 a,1 | 0.025 | 0.72 ab,1,2 | 0.029 | 0.66 b,2,3 | 0.025 | 0.61 3 | 0.029 |
c. Effects of dietary treatments on microbial diversity (Shannon index) at days 7, 14, 28, and 42. | ||||||||
Groups/Time | Mean Day-7 | SEM | Mean Day-14 | SEM | Mean Day-28 | SEM | Mean Day-42 | SEM |
NC | 5.30 a,1 | 0.218 | 4.39 bcd,2 | 0.244 | 5.67 1 | 0.218 | 5.46 ab,1 | 0.244 |
PC | 4.24 b,2 | 0.218 | 4.25 cd,2 | 0.244 | 5.77 1 | 0.218 | 4.69 ab,2 | 0.244 |
BPL | 5.25 a,1 | 0.244 | 3.88 d,2 | 0.244 | 5.81 1 | 0.244 | 5.69 a,1 | 0.244 |
BPH | 4.67 ab,2 | 0.218 | 5.66 a,1 | 0.218 | 5.17 1,2 | 0.218 | 4.89 ab,1,2 | 0.244 |
BSL | 5.37 a | 0.218 | 5.04 abc | 0.244 | 5.59 | 0.218 | 5.02 ab | 0.218 |
BSH | 5.39 a | 0.218 | 5.36 ab | 0.244 | 5.11 | 0.218 | 4.65 b | 0.244 |
a. Pairwise microbial dissimilarity between groups at day 7. | ||||||
Treatment Groups | NC | PC | BPL | BPH | BSL | BSH |
NC | 1 | 0.04 | 0.04 | 0.03 | 0.04 | 0.04 |
PC | 1 | 0.04 | 0.03 | 0.04 | 0.04 | |
BPL | 1 | 0.03 | 0.04 | 0.04 | ||
BPH | 1 | 0.03 | 0.06 | |||
BSL | 1 | 0.04 | ||||
BSH | 1 | |||||
b. Pairwise microbial dissimilarity between groups at day 14. | ||||||
Treatment Groups | NC | PC | BPL | BPH | BSL | BSH |
NC | 1 | 0.07 | 0.07 | 0.04 | 0.07 | 0.03 |
PC | 1 | 0.06 | 0.03 | 0.08 | 0.04 | |
BPL | 1 | 0.03 | 0.06 | 0.03 | ||
BPH | 1 | 0.03 | 0.03 | |||
BSL | 1 | 0.03 | ||||
BSH | 1 | |||||
c. Pairwise microbial dissimilarity between groups at day 28 | ||||||
Treatment Groups | NC | PC | BPL | BPH | BSL | BSH |
NC | 1 | 0.24 | 0.03 | 0.06 | 0.03 | 0.03 |
PC | 1 | 0.18 | 0.14 | 0.11 | 0.14 | |
BPL | 1 | 0.24 | 0.04 | 0.04 | ||
BPH | 1 | 0.24 | 0.79 | |||
BSL | 1 | 0.09 | ||||
BSH | 1 | |||||
d. Pairwise microbial dissimilarity between groups at day 42. | ||||||
Treatment Groups | NC | PC | BPL | BPH | BSL | BSH |
NC | 1 | 0.04 | 0.29 | 0.13 | 0.13 | 0.14 |
PC | 1 | 0.04 | 0.22 | 0.17 | 0.64 | |
BPL | 1 | 0.56 | 0.13 | 0.41 | ||
BPH | 1 | 0.28 | 0.56 | |||
BSL | 1 | 0.78 | ||||
BSH | 1 | |||||
e. Pairwise microbial dissimilarity within groups between days 7 and 14, 14 and 28, and 28 and 42. | ||||||
Groups/Time Points | Day 7 vs. Day 14 | Day 14 vs. Day 28 | Day 28 vs. Day 42 | |||
NC | 0.04 | 0.03 | 0.03 | |||
PC | 0.03 | 0.14 | 0.03 | |||
BPL | 0.05 | 0.04 | 0.27 | |||
BPH | 0.04 | 0.03 | 0.52 | |||
BSL | 0.03 | 0.03 | 0.03 | |||
BSH | 0.03 | 0.05 | 0.53 |
a. Effects of dietary treatments on the relative abundance of cecal microbiota at days 7 and 14. | |||||||||||||
Treatments (Day-7) 1 | Treatments (Day-14) 1 | ||||||||||||
Family | Genus | PC | BPH | BPL | BSH | BSL | NC | PC | BPH | BPL | BSH | BSL | NC |
Ruminococcaceae | Ruminococcus | −2.9 | −3.5 | −2.7 | −3.0 | −3.5 | −2.5 | −3.5 b | −2.7 a | −2.6 a | −2.7 a | −2.6 a | −3.6 b |
Oscillospira | −4.3 d | −3.8 c | −2.9 a | −3.4 b | −2.8 a | −3.5 bc | −3.9 bc | −3.5 abc | −2.9 a | −2.8 a | −3.2 ab | −4.1 c | |
Faecalibacterium | −6.4 b | −1.2a | −6.0 b | −6.9 b | −6.5 b | −6.3 b | −0.7 a | −3.7 cd | −2.9 b | −3.6 c | −4.0 d | −6.2 e | |
Butyricicoccus | −4.2 b | −4.8 b | * | −2.2 a | −4.6 b | −4.4 b | −6.5 c | −4.2 ab | −4.4 ab | −3.7 a | −4.0 a | −5.1 b | |
Subdoligranulum | * | * | * | * | * | * | −7.0 c | −3.3 b | 0.28 a | −2.7 b | −4.2 b | −3.4 b | |
Lachnospiraceae | Ruminococcus | 0.15 | −0.21 | 0.24 | −0.01 | 0.29 | −0.05 | −0.1 d | 0.3 c | −2.0 e | −0.1 d | 0.9 b | 1.7 a |
Blautia | −0.9 b | −1.6 a | −1.4 a | −0.4 bc | −0.3 c | −0.7 bc | −2.7 c | −1.2 b | −2.7 c | −0.7 ab | 0.1 a | −0.1 a | |
Coprococcus | −0.6 b | −2.5 b | −1.3 b | −0.9 b | −0.7 b | −1.6 ab | −2.6 c | −1.0 b | −3.7 d | −1.5 b | −1.1 b | −0.4 a | |
Dorea | −3.4 | −4.3 | −4.1 | −3.4 | −3.6 | −4.3 | −5.2 d | −3.4 b | −2.6 a | −3.8 bc | −4.4 c | −4.1 c | |
Clostridium | −5.4 | −6.1 | −5.4 | −5.9 | −5.7 | −5.4 | −5.3 a | −6.1 ab | −7.3 b | −4.8 a | −6.5 ab | −5.2 a | |
Lactobacillaceae | Lactobacillus | −1.01 | −1.81 | −0.04 | −1.18 | −0.74 | −1.08 | * | −2.7 b | −4.8 c | −0.9 a | −5.1 c | −5.2 c |
2 Erysipelotrichaceae | - | −2.6 | −3.2 | −2.3 | −1.9 | −2.0 | −2.6 | −4.0 b | −2.5 a | −4.4 b | −2.7 a | −2.6 a | −2.8 a |
Enterococcaceae | Enterococcus | −0.4 ab | −1.8 abc | 0.2 a | −1.8 bc | −3.0 c | −1.2 a–c | −3.3 a | −4.5 b | −5.2 c | −4.4 b | −6.0 d | −4.0 b |
Bifidobacteriaceae | Bifidobacterium | * | * | * | * | * | * | −6.7 b | −6.6 b | −0.6 a | −3.9 b | −5.6 b | −4.6 b |
Alcaligenaceae | Sutterella | * | * | * | * | * | * | −2.2 ab | −4.0 ab | −4.0 ab | −5.0 bc | −7.2 c | −1.0 a |
2 Enterobacteriaceae | - | −0.7a | −3.7c | −2.8 bc | −2.9 bc | −3.7c | −1.9 b | −1.6 a | −5.3 c | −5.0 c | −5.6 c | −2.9 b | −2.5 b |
b. Effects of dietary treatments on relative abundance of cecal microbiota at days 28 and 42. | |||||||||||||
Treatments (Day-28) 1 | Treatments (Day-42) 1 | ||||||||||||
Family | Genus | PC | BPH | BPL | BSH | BSL | NC | PC | BPH | BPL | BSH | BSL | NC |
Ruminococcaceae | Ruminococcus | −1.8 ab | −1.8 ab | −1.1a | −2.1 b | −2.6 b | −2.3 b | −3.0 b | −2.6 ab | −2.1 a | −2.9 b | −2.8 b | −3.0 b |
Oscillospira | −2.7a | −2.6a | −2.4a | −2.5a | −2.8a | −3.4 b | −2.6 | −2.9 | −2.6 | −3.2 | −2.6 | −2.8 | |
Faecalibacterium | −1.66 b | 0.03a | −0.68 ab | −0.07a | −0.46 ab | −1.7 b | 0.6 a | 0.2 ab | −0.4 b | 0.3 ab | 0.5 ab | 0.01 ab | |
Butyricicoccus | −4.0 | −3.8 | −4.5 | −4.5 | −3.6 | −4.5 | −4.9 ab | −4.3 ab | −3.6 a | −5.1 b | −4.6 ab | −3.9 ab | |
Subdoligranulum | −4.1 | −4.8 | −4.5 | −4.6 | −6.3 | −4.9 | −4.1 ab | −5.7 b | −5.1 b | −2.8 a | −5.3 b | −5.2 b | |
Lachnospiraceae | Ruminococcus | −0.9 | −1.7 | −1.2 | −1.3 | −1.2 | −0.5 | −1.7 ab | −1.3 a | −1.3 a | −1.6 ab | −2.1 b | −1.6 ab |
Blautia | −1.5 ab | −2.9 c | −1.2 ab | −2.5bc | −1.4 ab | −1.0 a | −2.9 ab | −2.4 ab | −2.0 a | −2.5 ab | −2.6 ab | −3.2 b | |
Coprococcus | −2.6bc | −3.3c | −2.1 ab | −2.2 ab | −2.5b | −1.7 a | −3.5 b | −3.4 b | −2.4 ab | −3.0 ab | −3.5 b | −2.3 a | |
Dorea | −3.8 | −3.6 | −3.6 | −3.7 | −3.3 | −3.6 | −3.1 a | −4.7 b | −3.8 ab | −4.4 b | −3.1 a | −4.3 b | |
Clostridium | −3.9 | −4.5 | −4.2 | −4.5 | −4.2 | −4.1 | −4.3 | −3.8 | −3.6 | −4.2 | −3.5 | −3.5 | |
Lactobacillaceae | Lactobacillus | −4.5 | −3.5 | −4.1 | −4.6 | −3.9 | −3.4 | −4.4 b | −2.2 a | −2.3 a | −4.0 ab | −4.5 b | −3.6 ab |
2 Erysipelotrichaceae | - | −3.1 | −3.9 | −3.8 | −3.6 | −3.2 | −3.5 | −4.6 | −3.1 | −3.2 | −4.1 | −3.8 | −3.9 |
Enterococcaceae | Enterococcus | −4.1 | −5.6 | −5.3 | −5.6 | −5.2 | −3.9 | −5.5 ab | −5.7 ab | −4.5 a | −6.8 b | −6.1 ab | −6.4 b |
Bifidobacteriaceae | Bifidobacterium | −2.4 | −6.0 | −5.4 | −5.0 | −4.1 | −3.1 | −3.0 | −5.1 | −3.0 | −2.4 | −2.1 | −1.9 |
Alcaligenaceae | Sutterella | −3.7c | −3.7c | −1.9 ab | −3.4c | −2.8bc | −1.4 a | −4.5 c | −3.4 bc | −2.4 ab | −3.8 c | −3.8 c | −1.7 a |
2 Enterobacteriaceae | - | −1.2a | −3.0 b | −4.2 cd | −5.1d | −4.3 cd | −3.8 bc | −4.5 | −5.6 | −3.8 | −4.3 | −4.7 | −5.1 |
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Bilal, M.; Achard, C.; Barbe, F.; Chevaux, E.; Ronholm, J.; Zhao, X. Bacillus pumilus and Bacillus subtilis Promote Early Maturation of Cecal Microbiota in Broiler Chickens. Microorganisms 2021, 9, 1899. https://doi.org/10.3390/microorganisms9091899
Bilal M, Achard C, Barbe F, Chevaux E, Ronholm J, Zhao X. Bacillus pumilus and Bacillus subtilis Promote Early Maturation of Cecal Microbiota in Broiler Chickens. Microorganisms. 2021; 9(9):1899. https://doi.org/10.3390/microorganisms9091899
Chicago/Turabian StyleBilal, Muhammad, Caroline Achard, Florence Barbe, Eric Chevaux, Jennifer Ronholm, and Xin Zhao. 2021. "Bacillus pumilus and Bacillus subtilis Promote Early Maturation of Cecal Microbiota in Broiler Chickens" Microorganisms 9, no. 9: 1899. https://doi.org/10.3390/microorganisms9091899