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Article

A Genomic and Transcriptomic Study on the DDT-Resistant Trichoderma hamatum FBL 587: First Genetic Data into Mycoremediation Strategies for DDT-Polluted Sites

1
Department of Technological Innovations and Safety of Plants, Products and Anthropic Settlements (DIT), INAIL, Research Area, Via R. Ferruzzi 38/40, 00143 Rome, Italy
2
Department of Environmental Biology, Sapienza University of Rome, P.le A. Moro 5, 00185 Rome, Italy
3
Council of Agricultural Research and Economics, Centre for Agriculture and Environment, Via Della Navicella 2/4, 00184 Rome, Italy
4
The National Institute of Horticultural Research, ul. Konstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Lisa M. Gieg
Microorganisms 2021, 9(8), 1680; https://doi.org/10.3390/microorganisms9081680
Received: 3 July 2021 / Revised: 1 August 2021 / Accepted: 4 August 2021 / Published: 7 August 2021
(This article belongs to the Special Issue Fungal Biodiversity for Bioremediation)
Trichoderma hamatum FBL 587 isolated from DDT-contaminated agricultural soils stands out as a remarkable strain with DDT-resistance and the ability to enhance DDT degradation process in soil. Here, whole genome sequencing and RNA-Seq studies for T. hamatum FBL 587 under exposure to DDT were performed. In the 38.9 Mb-genome of T. hamatum FBL 587, 10,944 protein-coding genes were predicted and annotated, including those of relevance to mycoremediation such as production of secondary metabolites and siderophores. The genome-scale transcriptional responses of T. hamatum FBL 587 to DDT exposure showed 1706 upregulated genes, some of which were putatively involved in the cellular translocation and degradation of DDT. With regards to DDT removal capacity, it was found upregulation of metabolizing enzymes such as P450s, and potentially of downstream DDT-transforming enzymes such as epoxide hydrolases, FAD-dependent monooxygenases, glycosyl- and glutathione-transferases. Based on transcriptional responses, the DDT degradation pathway could include transmembrane transporters of DDT, antioxidant enzymes for oxidative stress due to DDT exposure, as well as lipases and biosurfactants for the enhanced solubility of DDT. Our study provides the first genomic and transcriptomic data on T. hamatum FBL 587 under exposure to DDT, which are a base for a better understanding of mycoremediation strategies for DDT-polluted sites. View Full-Text
Keywords: bioremediation; carbohydrate active enzymes (CAZymes); DDT biodegradation; fungal genomics; genome-scale RNA-Seq; Hypocreaceae; secondary metabolites; siderophores; Trichoderma hamatum; whole genome sequencing bioremediation; carbohydrate active enzymes (CAZymes); DDT biodegradation; fungal genomics; genome-scale RNA-Seq; Hypocreaceae; secondary metabolites; siderophores; Trichoderma hamatum; whole genome sequencing
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MDPI and ACS Style

Davolos, D.; Russo, F.; Canfora, L.; Malusà, E.; Tartanus, M.; Furmanczyk, E.M.; Ceci, A.; Maggi, O.; Persiani, A.M. A Genomic and Transcriptomic Study on the DDT-Resistant Trichoderma hamatum FBL 587: First Genetic Data into Mycoremediation Strategies for DDT-Polluted Sites. Microorganisms 2021, 9, 1680. https://doi.org/10.3390/microorganisms9081680

AMA Style

Davolos D, Russo F, Canfora L, Malusà E, Tartanus M, Furmanczyk EM, Ceci A, Maggi O, Persiani AM. A Genomic and Transcriptomic Study on the DDT-Resistant Trichoderma hamatum FBL 587: First Genetic Data into Mycoremediation Strategies for DDT-Polluted Sites. Microorganisms. 2021; 9(8):1680. https://doi.org/10.3390/microorganisms9081680

Chicago/Turabian Style

Davolos, Domenico, Fabiana Russo, Loredana Canfora, Eligio Malusà, Małgorzata Tartanus, Ewa Maria Furmanczyk, Andrea Ceci, Oriana Maggi, and Anna Maria Persiani. 2021. "A Genomic and Transcriptomic Study on the DDT-Resistant Trichoderma hamatum FBL 587: First Genetic Data into Mycoremediation Strategies for DDT-Polluted Sites" Microorganisms 9, no. 8: 1680. https://doi.org/10.3390/microorganisms9081680

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