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Microorganisms
Volume 9
Issue 8
10.3390/microorganisms9081664
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Article

Development of a Novel Method for Identification of Alaria alata Mesocercariae by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

by
Carolyn Kästner
1,
Peter Bahn
1,
Ralph Schönfelder
2,
Zanda Ozoliņa
3,
Laura Alksne
3,
Martin Heinrich Richter
1,
Gunita Deksne
3,4,
Anne Mayer-Scholl
1 and
Annette Johne
1,*
1
Department for Biological Safety, German Federal Institute for Risk Assessment (BfR), 12277 Berlin, Germany
2
Food Inspection and Veterinary Department, Administrative District Görlitz, 02708 Löbau, Germany
3
Institute of Food Safety, Animal Health and Environment (BIOR), LV-1076 Riga, Latvia
4
Faculty of Biology, University of Latvia, LV-1004 Riga, Latvia
*
Author to whom correspondence should be addressed.
Academic Editor: Chao-Nan Lin
Microorganisms 2021, 9(8), 1664; https://doi.org/10.3390/microorganisms9081664
Received: 24 June 2021 / Revised: 28 July 2021 / Accepted: 29 July 2021 / Published: 4 August 2021
(This article belongs to the Topic Veterinary Infectious Diseases)
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Abstract

Alaria (A.) alata mesocercariae (AM) have increasingly appeared as incidental findings during the mandatory inspection of wild boars for Trichinella in many European countries. An Alaria spp.-specific PCR is available for the identification of AM; however, it is time- and cost-intensive. Therefore, we propose a rapid and cost-efficient MALDI-TOF assay for the identification of AM in wild boar meat that can be applied in routine diagnostics. In this study, a fast and methodologically simple protocol for the protein extraction of AM from different host species in different countries was established, and an AM-specific reference spectra database was created as part of the ongoing development of an existing Trichinella spp. database. A formic acid protein extraction was performed after pooling 10 AM from the same host individual. In total, 61 main spectra profiles (MSPs) from different host individuals were stored in an AM-specific MSP library. The cluster analysis of these 61 MSPs indicated a possible variation within the A. alata species with a tentative association with the geographical origin of the host, but not the host species. This MALDI-TOF assay allows for a fast verification of the AM isolates, which is the next step in the development of a universal database for the identification of several parasites isolated from meat. View Full-Text
Keywords: Alaria alata; MALDI-TOF MS; diagnostics; detection; identification; trematodes; wild boars; foodborne parasitology Alaria alata; MALDI-TOF MS; diagnostics; detection; identification; trematodes; wild boars; foodborne parasitology
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MDPI and ACS Style

Kästner, C.; Bahn, P.; Schönfelder, R.; Ozoliņa, Z.; Alksne, L.; Richter, M.H.; Deksne, G.; Mayer-Scholl, A.; Johne, A. Development of a Novel Method for Identification of Alaria alata Mesocercariae by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. Microorganisms 2021, 9, 1664. https://doi.org/10.3390/microorganisms9081664

AMA Style

Kästner C, Bahn P, Schönfelder R, Ozoliņa Z, Alksne L, Richter MH, Deksne G, Mayer-Scholl A, Johne A. Development of a Novel Method for Identification of Alaria alata Mesocercariae by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. Microorganisms. 2021; 9(8):1664. https://doi.org/10.3390/microorganisms9081664

Chicago/Turabian Style

Kästner, Carolyn, Peter Bahn, Ralph Schönfelder, Zanda Ozoliņa, Laura Alksne, Martin H. Richter, Gunita Deksne, Anne Mayer-Scholl, and Annette Johne. 2021. "Development of a Novel Method for Identification of Alaria alata Mesocercariae by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry" Microorganisms 9, no. 8: 1664. https://doi.org/10.3390/microorganisms9081664

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