Next Article in Journal
Metatranscriptomic Analysis of Bacterial Communities on Laundered Textiles: A Pilot Case Study
Next Article in Special Issue
Genetic Diversity of Leuconostoc mesenteroides Isolates from Traditional Montenegrin Brine Cheese
Previous Article in Journal
Molecular Phylogeny of Unicellular Marine Coccoid Green Algae Revealed New Insights into the Systematics of the Ulvophyceae (Chlorophyta)
Previous Article in Special Issue
Analysis and Reconstitution of the Menaquinone Biosynthesis Pathway in Lactiplantibacillus plantarum and Lentilactibacillus buchneri
Article

Oligosaccharide Metabolism and Lipoteichoic Acid Production in Lactobacillus gasseri and Lactobacillus paragasseri

1
Department of Microbiology, Sapporo Medical University School of Medicine, Sapporo 060-8556, Hokkaido, Japan
2
Department of Food, Aroma and Cosmetic Chemistry, Faculty of Bioindustry, Tokyo University of Agriculture, Abashiri 099-2493, Hokkaido, Japan
3
B Food Science Co., Ltd., Chita 478-0046, Aichi, Japan
4
Center for Information Biology, National Institute of Genetics, Mishima 411-8540, Shizuoka, Japan
5
RIKEN Center for Sustainable Resource Science, Yokohama 230-0045, Kanagawa, Japan
*
Authors to whom correspondence should be addressed.
Academic Editor: Franca Rossi
Microorganisms 2021, 9(8), 1590; https://doi.org/10.3390/microorganisms9081590
Received: 5 July 2021 / Revised: 20 July 2021 / Accepted: 20 July 2021 / Published: 26 July 2021
(This article belongs to the Special Issue Functional Characterization of Lactic Acid Bacteria)
Lactobacillus gasseri and Lactobacillus paragasseri are human commensal lactobacilli that are candidates for probiotic application. Knowledge of their oligosaccharide metabolic properties is valuable for synbiotic application. The present study characterized oligosaccharide metabolic systems and their impact on lipoteichoic acid (LTA) production in the two organisms, i.e., L. gasseri JCM 1131T and L. paragasseri JCM 11657. The two strains grew well in medium with glucose but poorly in medium with raffinose, and growth rates in medium with kestose differed between the strains. Oligosaccharide metabolism markedly influenced their LTA production, and apparent molecular size of LTA in electrophoresis recovered from cells cultured with glucose and kestose differed from that from cells cultured with raffinose in the strains. On the other hand, more than 15-fold more LTA was observed in the L. gasseri cells cultured with raffinose when compared with glucose or kestose after incubation for 15 h. Transcriptome analysis identified glycoside hydrolase family 32 enzyme as a potential kestose hydrolysis enzyme in the two strains. Transcriptomic levels of multiple genes in the dlt operon, involved in D-alanine substitution of LTA, were lower in cells cultured with raffinose than in those cultured with kestose or glucose. This suggested that the different sizes of LTA observed among the carbohydrates tested were partly due to different levels of alanylation of LTA. The present study indicates that available oligosaccharide has the impact on the LTA production of the industrially important lactobacilli, which might influence their probiotic properties. View Full-Text
Keywords: Lactobacillus gasseri; Lactobacillus paragasseri; kestose; lipoteichoic acid; glycoside hydrolase; RNAseq Lactobacillus gasseri; Lactobacillus paragasseri; kestose; lipoteichoic acid; glycoside hydrolase; RNAseq
Show Figures

Figure 1

MDPI and ACS Style

Shiraishi, T.; Maeno, S.; Kishi, S.; Fujii, T.; Tanno, H.; Hirano, K.; Tochio, T.; Tanizawa, Y.; Arita, M.; Yokota, S.-i.; Endo, A. Oligosaccharide Metabolism and Lipoteichoic Acid Production in Lactobacillus gasseri and Lactobacillus paragasseri. Microorganisms 2021, 9, 1590. https://doi.org/10.3390/microorganisms9081590

AMA Style

Shiraishi T, Maeno S, Kishi S, Fujii T, Tanno H, Hirano K, Tochio T, Tanizawa Y, Arita M, Yokota S-i, Endo A. Oligosaccharide Metabolism and Lipoteichoic Acid Production in Lactobacillus gasseri and Lactobacillus paragasseri. Microorganisms. 2021; 9(8):1590. https://doi.org/10.3390/microorganisms9081590

Chicago/Turabian Style

Shiraishi, Tsukasa, Shintaro Maeno, Sayoko Kishi, Tadashi Fujii, Hiroki Tanno, Katsuaki Hirano, Takumi Tochio, Yasuhiro Tanizawa, Masanori Arita, Shin-ichi Yokota, and Akihito Endo. 2021. "Oligosaccharide Metabolism and Lipoteichoic Acid Production in Lactobacillus gasseri and Lactobacillus paragasseri" Microorganisms 9, no. 8: 1590. https://doi.org/10.3390/microorganisms9081590

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop