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Article

Genome and Secretome Analysis of Staphylotrichum longicolleum DSM105789 Cultured on Agro-Residual and Chitinous Biomass †

1
Institute of Biochemistry and Molecular Biology, University of Hamburg, Martin Luther King Platz 6, 20146 Hamburg, Germany
2
Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, University Road, Karachi 75270, Pakistan
3
Institute of Clinical Chemistry and Laboratory Medicine, Diagnostic Center, Section Mass Spectrometry & Proteomics, Campus Research, Martinistr. 2, N27, Medical Center Hamburg-Eppendorf, Universität Hamburg, 20246 Hamburg, Germany
4
Department ScreeningPort, Fraunhofer Institute for Translational Medicine and Pharmacology ITMP, Schnackenburgallee 114, 22525 Hamburg, Germany
5
Department of Molecular Phytopathology, Biocenter Klein Flottbek, University of Hamburg, Ohnhorststr. 18, 22609 Hamburg, Germany
6
Institute of Food Chemistry and Food Biotechnology, Department Biology and Chemistry, Justus Liebig University Giessen, Heinrich-Buff-Ring 17, 35392 Gießen, Germany
7
Biomedical Science Institute, University of São Paulo, Av. Lineu Prestes, 2415, São Paulo CEP 05508-900, Brazil
*
Author to whom correspondence should be addressed.
This article is dedicated to Peter Grunwald and Frieder Schauer—both were outstanding scientists.
Academic Editors: Annett Mikolasch and Mariam Gaid
Microorganisms 2021, 9(8), 1581; https://doi.org/10.3390/microorganisms9081581
Received: 10 June 2021 / Revised: 19 July 2021 / Accepted: 20 July 2021 / Published: 25 July 2021
Staphylotrichum longicolleum FW57 (DSM105789) is a prolific chitinolytic fungus isolated from wood, with a chitinase activity of 0.11 ± 0.01 U/mg. We selected this strain for genome sequencing and annotation, and compiled its growth characteristics on four different chitinous substrates as well as two agro-industrial waste products. We found that the enzymatic mixture secreted by FW57 was not only able to digest pre-treated sugarcane bagasse, but also untreated sugarcane bagasse and maize leaves. The efficiency was comparable to a commercial enzymatic cocktail, highlighting the potential of the S. longicolleum enzyme mixture as an alternative pretreatment method. To further characterize the enzymes, which efficiently digested polymers such as cellulose, hemicellulose, pectin, starch, and lignin, we performed in-depth mass spectrometry-based secretome analysis using tryptic peptides from in-gel and in-solution digestions. Depending on the growth conditions, we were able to detect from 442 to 1092 proteins, which were annotated to identify from 134 to 224 putative carbohydrate-active enzymes (CAZymes) in five different families: glycoside hydrolases, auxiliary activities, carbohydrate esterases, polysaccharide lyases, glycosyl transferases, and proteins containing a carbohydrate-binding module, as well as combinations thereof. The FW57 enzyme mixture could be used to replace commercial enzyme cocktails for the digestion of agro-residual substrates. View Full-Text
Keywords: Staphylotrichum longicolleum; genome analysis; secretome; mass spectrometry; CAZyme analysis; chitin degradation; residual biomass treatment Staphylotrichum longicolleum; genome analysis; secretome; mass spectrometry; CAZyme analysis; chitin degradation; residual biomass treatment
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MDPI and ACS Style

Ali, A.; Ellinger, B.; Brandt, S.C.; Betzel, C.; Rühl, M.; Wrenger, C.; Schlüter, H.; Schäfer, W.; Brognaro, H.; Gand, M. Genome and Secretome Analysis of Staphylotrichum longicolleum DSM105789 Cultured on Agro-Residual and Chitinous Biomass. Microorganisms 2021, 9, 1581. https://doi.org/10.3390/microorganisms9081581

AMA Style

Ali A, Ellinger B, Brandt SC, Betzel C, Rühl M, Wrenger C, Schlüter H, Schäfer W, Brognaro H, Gand M. Genome and Secretome Analysis of Staphylotrichum longicolleum DSM105789 Cultured on Agro-Residual and Chitinous Biomass. Microorganisms. 2021; 9(8):1581. https://doi.org/10.3390/microorganisms9081581

Chicago/Turabian Style

Ali, Arslan, Bernhard Ellinger, Sophie C. Brandt, Christian Betzel, Martin Rühl, Carsten Wrenger, Hartmut Schlüter, Wilhelm Schäfer, Hévila Brognaro, and Martin Gand. 2021. "Genome and Secretome Analysis of Staphylotrichum longicolleum DSM105789 Cultured on Agro-Residual and Chitinous Biomass" Microorganisms 9, no. 8: 1581. https://doi.org/10.3390/microorganisms9081581

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