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Communication

Nearly Identical Plasmids Encoding VIM-1 and Mercury Resistance in Enterobacteriaceae from North-Eastern Germany

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Pharmaceutical Microbiology, University of Greifswald, 17489 Greifswald, Germany
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MVZ Laboratory Limbach Vorpommern-Rügen, 18435 Stralsund, Germany
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Klinische Hygiene und Infektiologie, Helios Hanseklinikum, 18435 Stralsund, Germany
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IMD Laboratory Greifswald, Institute of Medical Diagnostics, 17493 Greifswald, Germany
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Centogene, 18055 Rostock, Germany
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Central Unit for Infection Prevention and Control, University Medicine Greifswald, 17475 Greifswald, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Elisabeth Grohmann
Microorganisms 2021, 9(7), 1345; https://doi.org/10.3390/microorganisms9071345
Received: 23 April 2021 / Revised: 18 June 2021 / Accepted: 21 June 2021 / Published: 22 June 2021
(This article belongs to the Section Antimicrobial Agents and Resistance)
The emergence of carbapenemase-producing Enterobacteriaceae limits therapeutic options and presents a major public health problem. Resistances to carbapenems are mostly conveyed by metallo-beta-lactamases (MBL) including VIM, which are often encoded on resistance plasmids. We characterized four VIM-positive isolates that were obtained as part of a routine diagnostic screening from two laboratories in north-eastern Germany between June and August 2020. Whole-genome sequencing was performed to address (a) phylogenetic properties, (b) plasmid content, and (c) resistance gene carriage. In addition, we performed phenotypic antibiotic and mercury resistance analyses. The genomic analysis revealed three different bacterial species including C. freundii, E. coli and K. oxytoca with four different sequence types. All isolates were geno- and phenotypically multidrug-resistant (MDR) and the phenotypic profile was explained by the underlying resistance gene content. Three isolates of four carried nearly identical VIM-1-resistance plasmids, which in addition encoded a mercury resistance operon and showed some similarity to two publicly available plasmid sequences from sources other than the two laboratories above. Our results highlight the circulation of a nearly identical IncN-type VIM-1-resistance plasmid in different Enterobacteriaceae in north-eastern Germany. View Full-Text
Keywords: VIM; Enterobacteriaceae; mercury; resistance; IncN VIM; Enterobacteriaceae; mercury; resistance; IncN
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MDPI and ACS Style

Heiden, S.E.; Sydow, K.; Schaefer, S.; Klempien, I.; Balau, V.; Bauer, P.; Hübner, N.-O.; Schaufler, K. Nearly Identical Plasmids Encoding VIM-1 and Mercury Resistance in Enterobacteriaceae from North-Eastern Germany. Microorganisms 2021, 9, 1345. https://doi.org/10.3390/microorganisms9071345

AMA Style

Heiden SE, Sydow K, Schaefer S, Klempien I, Balau V, Bauer P, Hübner N-O, Schaufler K. Nearly Identical Plasmids Encoding VIM-1 and Mercury Resistance in Enterobacteriaceae from North-Eastern Germany. Microorganisms. 2021; 9(7):1345. https://doi.org/10.3390/microorganisms9071345

Chicago/Turabian Style

Heiden, Stefan E., Katharina Sydow, Stephan Schaefer, Ingo Klempien, Veronika Balau, Peter Bauer, Nils-Olaf Hübner, and Katharina Schaufler. 2021. "Nearly Identical Plasmids Encoding VIM-1 and Mercury Resistance in Enterobacteriaceae from North-Eastern Germany" Microorganisms 9, no. 7: 1345. https://doi.org/10.3390/microorganisms9071345

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