Next Article in Journal
Non-B DNA-Forming Motifs Promote Mfd-Dependent Stationary-Phase Mutagenesis in Bacillus subtilis
Next Article in Special Issue
Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) Analysis for the Identification of Pathogenic Microorganisms: A Review
Previous Article in Journal
From Klebsiella pneumoniae Colonization to Dissemination: An Overview of Studies Implementing Murine Models
Previous Article in Special Issue
What Do We See in Spectra?: Assignment of High-Intensity Peaks of Cutibacterium and Staphylococcus Spectra of MALDI-TOF Mass Spectrometry by Interspecies Comparative Proteogenomics
Review

MALDI-TOF MS in a Medical Mycology Laboratory: On Stage and Backstage

1
Centre National de la Recherche Scientifique, Centre Hospitalo-Universitaire Grenoble Alpes, Institut National de la Santé et de la Recherche Médicale, Institut Pour l’Avancée des Biosciences, University Grenoble Alpes, 38000 Grenoble, France
2
Translational Innovation in Medicine and Complexity, Centre National de la Recherche Scientifique, Centre Hospitalo-Universitaire Grenoble Alpes, Grenoble Alpes University, 38000 Grenoble, France
3
Charles Mérieux Infectiology Center, Campus Universitaire d’Ankatso, Antananarivo 101, Madagascar
4
Institut de Biologie Structurale, Commissariat à l’Énergie Atomique et aux Énergies Alternatives, Centre National de la Recherche Scientifique, Centre Hospitalo-Universitaire Grenoble Alpes, University Grenoble Alpes, 38000 Grenoble, France
5
Clinatec Departement, University Grenoble Alpes, Centre Hospitalo-Universitaire Grenoble Alpes, 38000 Grenoble, France
*
Author to whom correspondence should be addressed.
Academic Editor: Adriana Calderaro
Microorganisms 2021, 9(6), 1283; https://doi.org/10.3390/microorganisms9061283
Received: 4 May 2021 / Revised: 31 May 2021 / Accepted: 3 June 2021 / Published: 12 June 2021
The implementation of MALDI-TOF MS in medical microbiology laboratories has revolutionized practices and significantly reduced turnaround times of identification processes. However, although bacteriology quickly benefited from the contributions of this technique, adjustments were necessary to accommodate the specific characteristics of fungi. MALDI-TOF MS is now an indispensable tool in clinical mycology laboratories, both for the identification of yeasts and filamentous fungi, and other innovative uses are gradually emerging. Based on the practical experience of our medical mycology laboratory, this review will present the current uses of MALDI-TOF MS and the adaptations we implemented, to allow their practical execution in a daily routine. We will also introduce some less mainstream applications, like those for fungemia, or even still under development, as is the case for the determination of sensitivity to antifungal agents or typing methods. View Full-Text
Keywords: MALDI-TOF MS; medical mycology; diagnosis; microorganism identification; fungi; antifungal susceptibility testing MALDI-TOF MS; medical mycology; diagnosis; microorganism identification; fungi; antifungal susceptibility testing
Show Figures

Figure 1

MDPI and ACS Style

Robert, M.-G.; Cornet, M.; Hennebique, A.; Rasamoelina, T.; Caspar, Y.; Pondérand, L.; Bidart, M.; Durand, H.; Jacquet, M.; Garnaud, C.; Maubon, D. MALDI-TOF MS in a Medical Mycology Laboratory: On Stage and Backstage. Microorganisms 2021, 9, 1283. https://doi.org/10.3390/microorganisms9061283

AMA Style

Robert M-G, Cornet M, Hennebique A, Rasamoelina T, Caspar Y, Pondérand L, Bidart M, Durand H, Jacquet M, Garnaud C, Maubon D. MALDI-TOF MS in a Medical Mycology Laboratory: On Stage and Backstage. Microorganisms. 2021; 9(6):1283. https://doi.org/10.3390/microorganisms9061283

Chicago/Turabian Style

Robert, Marie-Gladys, Muriel Cornet, Aurélie Hennebique, Tahinamandranto Rasamoelina, Yvan Caspar, Léa Pondérand, Marie Bidart, Harmonie Durand, Marvin Jacquet, Cécile Garnaud, and Danièle Maubon. 2021. "MALDI-TOF MS in a Medical Mycology Laboratory: On Stage and Backstage" Microorganisms 9, no. 6: 1283. https://doi.org/10.3390/microorganisms9061283

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop