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Article

In Vitro Susceptibility to Miltefosine of Leishmania infantum (syn. L. chagasi) Isolates from Different Geographical Areas in Brazil

1
Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo 05508-000, Brazil
2
Laboratório de Pesquisa em Leishmanioses, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-900, Brazil
3
Universidade Federal do Piauí, Hospital de Doenças Tropicais Natan Portela, Teresina, Piauí 64002-510, Brazil
4
Drugs for Neglected Diseases initiative (DNDi), 1202 Geneva, Switzerland
5
Drugs for Neglected Diseases initiative (DNDi), Rio de Janeiro 20010-020, Brazil
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: David S. Lindsay
Microorganisms 2021, 9(6), 1228; https://doi.org/10.3390/microorganisms9061228
Received: 29 March 2021 / Revised: 22 April 2021 / Accepted: 2 June 2021 / Published: 5 June 2021
(This article belongs to the Special Issue Leishmania and Leishmaniasis)
Treatment of visceral leishmaniasis in Brazil still relies on meglumine antimoniate, with less than ideal efficacy and safety, making new therapeutic tools an urgent need. The oral drug miltefosine was assayed in a phase II clinical trial in Brazil with cure rates lower than previously demonstrated in India. The present study investigated the susceptibility to miltefosine in 73 Brazilian strains of Leishmania infantum from different geographic regions, using intracellular amastigote and promastigote assays. The EC50 for miltefosine of 13 of these strains evaluated in intracellular amastigotes varied between 1.41 and 4.57 μM. The EC50 of the 73 strains determined in promastigotes varied between 5.89 and 23.7 μM. No correlation between in vitro miltefosine susceptibility and the presence of the miltefosine sensitive locus was detected among the tested strains. The relatively low heterogeneity in miltefosine susceptibility observed for the 73 strains tested in this study suggests the absence of decreased susceptibility to miltefosine in Brazilian L. infantum and does not exclude future clinical evaluation of miltefosine for VL treatment in Brazil. View Full-Text
Keywords: clinical strains; miltefosine; susceptibility; visceral leishmaniasis clinical strains; miltefosine; susceptibility; visceral leishmaniasis
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MDPI and ACS Style

Espada, C.R.; Levatti, E.V.d.C.; Boité, M.C.; Lamounier, D.; Alvar, J.; Cupolillo, E.; Costa, C.H.N.; Rode, J.; Uliana, S.R.B. In Vitro Susceptibility to Miltefosine of Leishmania infantum (syn. L. chagasi) Isolates from Different Geographical Areas in Brazil. Microorganisms 2021, 9, 1228. https://doi.org/10.3390/microorganisms9061228

AMA Style

Espada CR, Levatti EVdC, Boité MC, Lamounier D, Alvar J, Cupolillo E, Costa CHN, Rode J, Uliana SRB. In Vitro Susceptibility to Miltefosine of Leishmania infantum (syn. L. chagasi) Isolates from Different Geographical Areas in Brazil. Microorganisms. 2021; 9(6):1228. https://doi.org/10.3390/microorganisms9061228

Chicago/Turabian Style

Espada, Caroline R., Erica V.d.C. Levatti, Mariana C. Boité, Dorcas Lamounier, Jorge Alvar, Elisa Cupolillo, Carlos H.N. Costa, Joelle Rode, and Silvia R.B. Uliana 2021. "In Vitro Susceptibility to Miltefosine of Leishmania infantum (syn. L. chagasi) Isolates from Different Geographical Areas in Brazil" Microorganisms 9, no. 6: 1228. https://doi.org/10.3390/microorganisms9061228

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