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Article

Identity of blaCTX-M Carrying Plasmids in Sequential ESBL-E. coli Isolates from Patients with Recurrent Urinary Tract Infections

1
Institute of Biomedicine, Department of Infectious Diseases and Centre for Antibiotic Resistance Research (CARe), University of Gothenburg, Guldhedsgatan 10 A, 413 46 Gothenburg, Sweden
2
Västra Götaland Region, Sahlgrenska University Hospital, Department of Clinical Microbiology, Guldhedsgatan 10A, 413 46 Gothenburg, Sweden
3
Department of Biology and Biological Engineering, Chalmers University of Technology, Kemivagen 10, 412 96 Gothenburg, Sweden
4
Swedish Strategic Program against Antimicrobial Resistance (Strama), Västra Götaland Region, Regionens Hus, 405 44 Gothenburg, Sweden
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Rémy A. Bonnin
Microorganisms 2021, 9(6), 1138; https://doi.org/10.3390/microorganisms9061138
Received: 5 April 2021 / Revised: 10 May 2021 / Accepted: 20 May 2021 / Published: 25 May 2021
(This article belongs to the Special Issue ß-Lactamases)
Plasmid-mediated multidrug resistance in E. coli is becoming increasingly prevalent. Considering this global threat to human health, it is important to understand how plasmid-mediated resistance spreads. From a cohort of 123 patients with recurrent urinary tract infections (RUTI) due to extended spectrum beta-lactamase (ESBL)-producing Escherichia coli (ESBL E. coli), only five events with a change of ESBL E. coli strain between RUTI episodes were identified. Their blaCTX-M encoding plasmids were compared within each pair of isolates using optical DNA mapping (ODM) and PCR-based replicon typing. Despite similar blaCTX-M genes and replicon types, ODM detected only one case with identical plasmids in the sequential ESBL E. coli strains, indicating that plasmid transfer could have occurred. For comparison, plasmids from seven patients with the same ESBL E. coli strain reoccurring in both episodes were analyzed. These plasmids (encoding blaCTX-M-3, blaCTX-M-14, and blaCTX-M-15) were unaltered for up to six months between recurrent infections. Thus, transmission of blaCTX-M plasmids appears to be a rare event during the course of RUTI. Despite the limited number (n = 23) of plasmids investigated, similar blaCTX-M-15 plasmids in unrelated isolates from different patients were detected, suggesting that some successful plasmids could be associated with specific strains, or are more easily transmitted. View Full-Text
Keywords: extended-spectrum beta-lactamase (ESBL); recurrent urinary tract infection; plasmids; horizontal transfer; E. coli; optical DNA mapping (ODM) extended-spectrum beta-lactamase (ESBL); recurrent urinary tract infection; plasmids; horizontal transfer; E. coli; optical DNA mapping (ODM)
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MDPI and ACS Style

Karami, N.; KK, S.; Yazdanshenas, S.; Lin, Y.-L.; Jaén-Luchoro, D.; Ekedahl, E.; Parameshwaran, S.; Lindblom, A.; Åhrén, C.; Westerlund, F. Identity of blaCTX-M Carrying Plasmids in Sequential ESBL-E. coli Isolates from Patients with Recurrent Urinary Tract Infections. Microorganisms 2021, 9, 1138. https://doi.org/10.3390/microorganisms9061138

AMA Style

Karami N, KK S, Yazdanshenas S, Lin Y-L, Jaén-Luchoro D, Ekedahl E, Parameshwaran S, Lindblom A, Åhrén C, Westerlund F. Identity of blaCTX-M Carrying Plasmids in Sequential ESBL-E. coli Isolates from Patients with Recurrent Urinary Tract Infections. Microorganisms. 2021; 9(6):1138. https://doi.org/10.3390/microorganisms9061138

Chicago/Turabian Style

Karami, Nahid, Sriram KK, Shora Yazdanshenas, Yii-Lih Lin, Daniel Jaén-Luchoro, Elina Ekedahl, Sanjana Parameshwaran, Anna Lindblom, Christina Åhrén, and Fredrik Westerlund. 2021. "Identity of blaCTX-M Carrying Plasmids in Sequential ESBL-E. coli Isolates from Patients with Recurrent Urinary Tract Infections" Microorganisms 9, no. 6: 1138. https://doi.org/10.3390/microorganisms9061138

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