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Article

Resistant Sources and Genetic Control of Resistance to ToLCNDV in Cucumber

Instituto de Conservación y Mejora de la Agrodiversidad Valenciana, Universitat Politècnica de València, Avenida de los Naranjos s/n, 46022 Valencia, Spain
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Academic Editor: Jesús Navas Castillo
Microorganisms 2021, 9(5), 913; https://doi.org/10.3390/microorganisms9050913
Received: 25 March 2021 / Revised: 20 April 2021 / Accepted: 21 April 2021 / Published: 24 April 2021
(This article belongs to the Special Issue Plant Viruses: From Ecology to Control)
Tomato leaf curl New Delhi virus (ToLCNDV) is a severe threat for cucurbit production worldwide. Resistance has been reported in several crops, but at present, there are no described accessions with resistance to ToLCNDV in cucumber (Cucumis sativus). C. sativus var. sativus accessions were mechanically inoculated with ToLCNDV and screened for resistance, by scoring symptom severity, tissue printing, and PCR (conventional and quantitative). Severe symptoms and high load of viral DNA were found in plants of a nuclear collection of Spanish landraces and in accessions of C. sativus from different geographical origins. Three Indian accessions (CGN23089, CGN23423, and CGN23633) were highly resistant to the mechanical inoculation, as well as all plants of their progenies obtained by selfing. To study the inheritance of the resistance to ToLCNDV, plants of the CGN23089 accession were crossed with the susceptible accession BGV011742, and F1 hybrids were used to construct segregating populations (F2 and backcrosses), which were mechanically inoculated and evaluated for symptom development and viral load by qPCR. The analysis of the genetic control fit with a recessive monogenic inheritance model, and after genotyping with SNPs distributed along the C. sativus genome, a QTL associated with ToLCNDV resistance was identified in chromosome 2 of cucumber. View Full-Text
Keywords: Begomovirus; cucumber; mechanical inoculation; real-time PCR; viral load; QTLs; resistance Begomovirus; cucumber; mechanical inoculation; real-time PCR; viral load; QTLs; resistance
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MDPI and ACS Style

Sáez, C.; Ambrosio, L.G.M.; Miguel, S.M.; Valcárcel, J.V.; Díez, M.J.; Picó, B.; López, C. Resistant Sources and Genetic Control of Resistance to ToLCNDV in Cucumber. Microorganisms 2021, 9, 913. https://doi.org/10.3390/microorganisms9050913

AMA Style

Sáez C, Ambrosio LGM, Miguel SM, Valcárcel JV, Díez MJ, Picó B, López C. Resistant Sources and Genetic Control of Resistance to ToLCNDV in Cucumber. Microorganisms. 2021; 9(5):913. https://doi.org/10.3390/microorganisms9050913

Chicago/Turabian Style

Sáez, Cristina, Laura G.M. Ambrosio, Silvia M. Miguel, José V. Valcárcel, María J. Díez, Belén Picó, and Carmelo López. 2021. "Resistant Sources and Genetic Control of Resistance to ToLCNDV in Cucumber" Microorganisms 9, no. 5: 913. https://doi.org/10.3390/microorganisms9050913

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