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Article

Cryptosporidium myocastoris n. sp. (Apicomplexa: Cryptosporidiidae), the Species Adapted to the Nutria (Myocastor coypus)

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Faculty of Agriculture, University of South Bohemia in České Budějovice, Studentská 1668, 37005 České Budějovice, Czech Republic
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Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branišovská 31, 37005 České Budějovice, Czech Republic
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Microbiological Sciences Department, North Dakota State University, 1523 Centennial Blvd, Van Es Hall, Fargo, ND 58102, USA
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Faculty of Forestry, Technical University in Zvolen, 960 01 Zvolen, Slovakia
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Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China
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Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China
*
Author to whom correspondence should be addressed.
Academic Editor: Andrea Bardůnek Valigurová
Microorganisms 2021, 9(4), 813; https://doi.org/10.3390/microorganisms9040813
Received: 9 March 2021 / Revised: 1 April 2021 / Accepted: 6 April 2021 / Published: 12 April 2021
(This article belongs to the Special Issue Parasitic Protists: Diversity of Adaptations to a Parasitic Lifestyle)
Cryptosporidium spp., common parasites of vertebrates, remain poorly studied in wildlife. This study describes the novel Cryptosporidium species adapted to nutrias (Myocastor coypus). A total of 150 faecal samples of feral nutria were collected from locations in the Czech Republic and Slovakia and examined for Cryptosporidium spp. oocysts and specific DNA at the SSU, actin, HSP70, and gp60 loci. Molecular analyses revealed the presence of C. parvum (n = 1), C. ubiquitum subtype family XIId (n = 5) and Cryptosporidium myocastoris n. sp. XXIIa (n = 2), and XXIIb (n = 3). Only nutrias positive for C. myocastoris shed microscopically detectable oocysts, which measured 4.8–5.2 × 4.7–5.0 µm, and oocysts were infectious for experimentally infected nutrias with a prepatent period of 5–6 days, although not for mice, gerbils, or chickens. The infection was localised in jejunum and ileum without observable macroscopic changes. The microvilli adjacent to attached stages responded by elongating. Clinical signs were not observed in naturally or experimentally infected nutrias. Phylogenetic analyses at SSU, actin, and HSP70 loci demonstrated that C. myocastoris n. sp. is distinct from other valid Cryptosporidium species. View Full-Text
Keywords: adaptation; prevalence; biology; course of infection; infectivity; oocyst size; phylogeny; parasite adaptation; prevalence; biology; course of infection; infectivity; oocyst size; phylogeny; parasite
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MDPI and ACS Style

Ježková, J.; Limpouchová, Z.; Prediger, J.; Holubová, N.; Sak, B.; Konečný, R.; Květoňová, D.; Hlásková, L.; Rost, M.; McEvoy, J.; Rajský, D.; Feng, Y.; Kváč, M. Cryptosporidium myocastoris n. sp. (Apicomplexa: Cryptosporidiidae), the Species Adapted to the Nutria (Myocastor coypus). Microorganisms 2021, 9, 813. https://doi.org/10.3390/microorganisms9040813

AMA Style

Ježková J, Limpouchová Z, Prediger J, Holubová N, Sak B, Konečný R, Květoňová D, Hlásková L, Rost M, McEvoy J, Rajský D, Feng Y, Kváč M. Cryptosporidium myocastoris n. sp. (Apicomplexa: Cryptosporidiidae), the Species Adapted to the Nutria (Myocastor coypus). Microorganisms. 2021; 9(4):813. https://doi.org/10.3390/microorganisms9040813

Chicago/Turabian Style

Ježková, Jana, Zlata Limpouchová, Jitka Prediger, Nikola Holubová, Bohumil Sak, Roman Konečný, Dana Květoňová, Lenka Hlásková, Michael Rost, John McEvoy, Dušan Rajský, Yaoyu Feng, and Martin Kváč. 2021. "Cryptosporidium myocastoris n. sp. (Apicomplexa: Cryptosporidiidae), the Species Adapted to the Nutria (Myocastor coypus)" Microorganisms 9, no. 4: 813. https://doi.org/10.3390/microorganisms9040813

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