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Article

Characterization of the Aerobic Anoxygenic Phototrophic Bacterium Sphingomonas sp. AAP5

1
Centre Algatech, Institute of Microbiology, Czech Academy of Sciences, 379 81 Třeboň, Czech Republic
2
Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark
3
Faculty of Science, University of South Bohemia, 370 05 České Budějovice, Czech Republic
4
Institute of Parasitology, Biology Centre, Czech Academy of Sciences, 370 05 České Budějovice, Czech Republic
5
Research Group Microbial Communication, Technical University of Braunschweig, 38106 Braunschweig, Germany
6
Laboratory of Aquatic Photobiology and Plankton Ecology, Department of Ecology, University of Innsbruck, 6020 Innsbruck, Austria
*
Author to whom correspondence should be addressed.
Present Address: Department of Molecular Bacteriology, Helmholtz-Centre for Infection Research, 38106 Braunschweig, Germany.
Academic Editor: Johannes F. Imhoff
Microorganisms 2021, 9(4), 768; https://doi.org/10.3390/microorganisms9040768
Received: 9 March 2021 / Revised: 31 March 2021 / Accepted: 5 April 2021 / Published: 6 April 2021
(This article belongs to the Special Issue Advances in the Biology of Phototrophic Bacteria)
An aerobic, yellow-pigmented, bacteriochlorophyll a-producing strain, designated AAP5 (=DSM 111157=CCUG 74776), was isolated from the alpine lake Gossenköllesee located in the Tyrolean Alps, Austria. Here, we report its description and polyphasic characterization. Phylogenetic analysis of the 16S rRNA gene showed that strain AAP5 belongs to the bacterial genus Sphingomonas and has the highest pairwise 16S rRNA gene sequence similarity with Sphingomonas glacialis (98.3%), Sphingomonas psychrolutea (96.8%), and Sphingomonas melonis (96.5%). Its genomic DNA G + C content is 65.9%. Further, in silico DNA-DNA hybridization and calculation of the average nucleotide identity speaks for the close phylogenetic relationship of AAP5 and Sphingomonas glacialis. The high percentage (76.2%) of shared orthologous gene clusters between strain AAP5 and Sphingomonas paucimobilis NCTC 11030T, the type species of the genus, supports the classification of the two strains into the same genus. Strain AAP5 was found to contain C18:1ω7c (64.6%) as a predominant fatty acid (>10%) and the polar lipid profile contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid, six unidentified glycolipids, one unidentified phospholipid, and two unidentified lipids. The main respiratory quinone was ubiquinone-10. Strain AAP5 is a facultative photoheterotroph containing type-2 photosynthetic reaction centers and, in addition, contains a xathorhodopsin gene. No CO2-fixation pathways were found. View Full-Text
Keywords: aerobic anoxygenic phototrophic bacteria; bacteriochlorophyll a; photosynthesis genes; rhodopsin; Sphingomonadaceae aerobic anoxygenic phototrophic bacteria; bacteriochlorophyll a; photosynthesis genes; rhodopsin; Sphingomonadaceae
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MDPI and ACS Style

Kopejtka, K.; Zeng, Y.; Kaftan, D.; Selyanin, V.; Gardian, Z.; Tomasch, J.; Sommaruga, R.; Koblížek, M. Characterization of the Aerobic Anoxygenic Phototrophic Bacterium Sphingomonas sp. AAP5. Microorganisms 2021, 9, 768. https://doi.org/10.3390/microorganisms9040768

AMA Style

Kopejtka K, Zeng Y, Kaftan D, Selyanin V, Gardian Z, Tomasch J, Sommaruga R, Koblížek M. Characterization of the Aerobic Anoxygenic Phototrophic Bacterium Sphingomonas sp. AAP5. Microorganisms. 2021; 9(4):768. https://doi.org/10.3390/microorganisms9040768

Chicago/Turabian Style

Kopejtka, Karel, Yonghui Zeng, David Kaftan, Vadim Selyanin, Zdenko Gardian, Jürgen Tomasch, Ruben Sommaruga, and Michal Koblížek. 2021. "Characterization of the Aerobic Anoxygenic Phototrophic Bacterium Sphingomonas sp. AAP5" Microorganisms 9, no. 4: 768. https://doi.org/10.3390/microorganisms9040768

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