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Ectopic Spacer Acquisition in Streptococcus thermophilus CRISPR3 Array

1
Laboratorio de Biotecnología, Facultad de Ingeniería, Universidad ORT Uruguay, Montevideo 11100, Uruguay
2
Département de Biochimie, De Microbiologie et de Bio-Informatique, Faculté des Sciences et de Génie, Université Laval, Québec, QC G1V 0A6, Canada
3
Groupe de Recherche en Écologie Buccale, Faculté de Médecine Dentaire, Université Laval, Québec, QC G1V 0A6, Canada
4
Departamento de Biociencias (DEPBIO), Facultad de Química, Universidad de la República, Montevideo 11800, Uruguay
5
Félix d’Hérelle Reference Center for Bacterial Viruses, Faculté de Médecine Dentaire, Université Laval, Québec, QC G1V 0A6, Canada
*
Author to whom correspondence should be addressed.
Academic Editor: Jennifer Mahony
Microorganisms 2021, 9(3), 512; https://doi.org/10.3390/microorganisms9030512
Received: 3 February 2021 / Revised: 24 February 2021 / Accepted: 24 February 2021 / Published: 1 March 2021
(This article belongs to the Special Issue Siphophages Infecting Food Processing Bacteria)
Streptococcus thermophilus relies heavily on two type II-A CRISPR-Cas systems, CRISPR1 and CRISPR3, to resist siphophage infections. One hallmark of these systems is the integration of a new spacer at the 5′ end of the CRISPR arrays following phage infection. However, we have previously shown that ectopic acquisition of spacers can occur within the CRISPR1 array. Here, we present evidence of the acquisition of new spacers within the array of CRISPR3 of S. thermophilus. The analysis of randomly selected bacteriophage-insensitive mutants of the strain Uy01 obtained after phage infection, as well as the comparison with other S. thermophilus strains with similar CRISPR3 content, showed that a specific spacer within the array could be responsible for misguiding the adaptation complex. These results also indicate that while the vast majority of new spacers are added at the 5′ end of the CRISPR array, ectopic spacer acquisition is a common feature of both CRISPR1 and CRISPR3 systems in S. thermophilus, and it can still provide phage resistance. Ectopic spacer acquisition also appears to have occurred naturally in some strains of Streptococcus pyogenes, suggesting that it is a general phenomenon, at least in type II-A systems. View Full-Text
Keywords: Streptococcus thermophilus; Streptococcus pyogenes; CRISPR-Cas; adaptation; phages Streptococcus thermophilus; Streptococcus pyogenes; CRISPR-Cas; adaptation; phages
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MDPI and ACS Style

Achigar, R.; Scarrone, M.; Rousseau, G.M.; Philippe, C.; Machado, F.; Duvós, V.; Campot, M.P.; Dion, M.B.; Shao, Y.; Pianzzola, M.J.; Moineau, S. Ectopic Spacer Acquisition in Streptococcus thermophilus CRISPR3 Array. Microorganisms 2021, 9, 512. https://doi.org/10.3390/microorganisms9030512

AMA Style

Achigar R, Scarrone M, Rousseau GM, Philippe C, Machado F, Duvós V, Campot MP, Dion MB, Shao Y, Pianzzola MJ, Moineau S. Ectopic Spacer Acquisition in Streptococcus thermophilus CRISPR3 Array. Microorganisms. 2021; 9(3):512. https://doi.org/10.3390/microorganisms9030512

Chicago/Turabian Style

Achigar, Rodrigo, Martina Scarrone, Geneviève M. Rousseau, Cécile Philippe, Felipe Machado, Valentina Duvós, María Pía Campot, Moïra B. Dion, Yuyu Shao, María Julia Pianzzola, and Sylvain Moineau. 2021. "Ectopic Spacer Acquisition in Streptococcus thermophilus CRISPR3 Array" Microorganisms 9, no. 3: 512. https://doi.org/10.3390/microorganisms9030512

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