Next Article in Journal
Impacts of Low Temperature and Ensiling Period on the Bacterial Community of Oat Silage by SMRT
Next Article in Special Issue
Antimicrobial Resistance Mechanisms and Virulence of Colistin- and Carbapenem-Resistant Acinetobacter baumannii Isolated from a Teaching Hospital in Taiwan
Previous Article in Journal
Acknowledgment to Reviewers of Microorganisms in 2020
Previous Article in Special Issue
Epidemiology and Characterization of CTX-M-55-Type Extended-Spectrum β-Lactamase-Producing Salmonella enterica Serovar Enteritidis Isolated from Patients in Shanghai, China
Article

Molecular Epidemiology and Characterization of Carbapenem-Resistant Klebsiella pneumoniae Isolated from Urine at a Teaching Hospital in Taiwan

1
Division of Infectious Diseases, Department of Internal Medicine, Taipei Medical University Hospital, Taipei 11031, Taiwan
2
Division of Infectious Diseases, Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan
3
Graduate Institute of Biochemical and Biomedical Engineering, National Taipei University of Technology, Taipei 10608, Taiwan
4
International Master/Ph.D. Program in Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan
5
Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan
6
Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan
*
Author to whom correspondence should be addressed.
Academic Editor: Matthew Gavino Donadu
Microorganisms 2021, 9(2), 271; https://doi.org/10.3390/microorganisms9020271
Received: 17 December 2020 / Revised: 22 January 2021 / Accepted: 22 January 2021 / Published: 28 January 2021
Urinary tract infections (UTIs) are common in clinics and hospitals and are associated with a high economic burden. Enterobacterium Klebsiella pneumoniae is a prevalent agent causing UTIs. A high prevalence of carbapenem-resistant K. pneumoniae (CRKP) has emerged recently and is continuing to increase. Seventeen urinary CRKP isolates collected at a teaching hospital in Taiwan from December 2016 to September 2017 were analyzed to elucidate their drug resistance mechanisms. Two-thirds of the isolates were obtained from outpatients. Antimicrobial susceptibility tests demonstrated multidrug resistance in all the isolates. Multilocus sequence typing analysis showed high diversity among the isolates. PCR analysis demonstrated the presence of carbapenemases in three isolates. All isolates carried at least one other extended-spectrum β-lactamase, including TEM, DHA, and CTX-M. Fifteen isolates contained mutations in one of the outer membrane porins that were assessed. The expression levels of the acrB and/or oqxB efflux pump genes, as determined by qRT-PCR, were upregulated in 11 isolates. Six isolates might have utilized other efflux pumps or antimicrobial resistance mechanisms. These analyses demonstrated a highly diverse population and the presence of complex resistance mechanisms in urinary isolates of K. pneumoniae. View Full-Text
Keywords: antimicrobial resistance; carbapenem-resistant mechanism; extended-spectrum β-lactamase; carbapenemase; urinary tract infection antimicrobial resistance; carbapenem-resistant mechanism; extended-spectrum β-lactamase; carbapenemase; urinary tract infection
Show Figures

Figure 1

MDPI and ACS Style

Lee, Y.-J.; Huang, C.-H.; Ilsan, N.A.; Lee, I.-H.; Huang, T.-W. Molecular Epidemiology and Characterization of Carbapenem-Resistant Klebsiella pneumoniae Isolated from Urine at a Teaching Hospital in Taiwan. Microorganisms 2021, 9, 271. https://doi.org/10.3390/microorganisms9020271

AMA Style

Lee Y-J, Huang C-H, Ilsan NA, Lee I-H, Huang T-W. Molecular Epidemiology and Characterization of Carbapenem-Resistant Klebsiella pneumoniae Isolated from Urine at a Teaching Hospital in Taiwan. Microorganisms. 2021; 9(2):271. https://doi.org/10.3390/microorganisms9020271

Chicago/Turabian Style

Lee, Yuarn-Jang, Chih-Hung Huang, Noor A. Ilsan, I-Hui Lee, and Tzu-Wen Huang. 2021. "Molecular Epidemiology and Characterization of Carbapenem-Resistant Klebsiella pneumoniae Isolated from Urine at a Teaching Hospital in Taiwan" Microorganisms 9, no. 2: 271. https://doi.org/10.3390/microorganisms9020271

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop