Next Article in Journal
Bioplastic Production by Bacillus wiedmannii AS-02 OK576278 Using Different Agricultural Wastes
Previous Article in Journal
Use of Sedatives and Neuromuscular-Blocking Agents in Mechanically Ventilated Patients with COVID-19 ARDS
Previous Article in Special Issue
Parasitic Intestinal Protists of Zoonotic Relevance Detected in Pigs by Metabarcoding and Real-Time PCR
Article

Cross-Border Investigations on the Prevalence and Transmission Dynamics of Cryptosporidium Species in Dairy Cattle Farms in Western Mainland Europe

1
Laboratory of Molecular and Evolutionary Parasitology, RAPID Group, School of Biosciences, University of Kent, Canterbury CT2 7NZ, UK
2
UMR-Transfrontalière 1158 BioEcoAgro, Junia, University of Lille, University of Liège, UPJV, ULCO, University of Artois, INRAE, F-59000 Lille, France
3
Junia, Comportement Animal et Systèmes d’Elevage, F-59000 Lille, France
4
University of Lille, Institut Mines-Télécom, University of Artois, Junia, ULR 4515—LGCgE, Laboratoire de Génie Civil et Géo-Environnement, F-59000 Lille, France
5
Inagro vzw, Ieperseweg 87, 8800 Rumbeke-Beitem, Belgium
6
Southern Agricultural and Horticultural Organisation (ZLTO), Onderwijsboulevard 225, 5223 DE’s-Hertogenbosch, The Netherlands
7
Selas, 31 Rue de la République, 59496 Salome, France
8
Biology Centre of the Academy of Sciences of the Czech Republic, Institute of Parasitology, 37005 České Budějovice, Czech Republic
9
Faculty of Agriculture, University of South Bohemia in České Budějovice, 37005 České Budějovice, Czech Republic
10
University of Lille, CNRS, Centrale Lille, Junia, University Polytechnique Hauts de France, UMR 8520 IEMN Institut d’Electronique de Microélectronique et de Nanotechnologie, F 59000 Lille, France
*
Author to whom correspondence should be addressed.
Academic Editor: Gereon R. M. Schares
Microorganisms 2021, 9(11), 2394; https://doi.org/10.3390/microorganisms9112394 (registering DOI)
Received: 15 October 2021 / Revised: 5 November 2021 / Accepted: 15 November 2021 / Published: 20 November 2021
(This article belongs to the Special Issue Evolution and Genetic Diversity of Gut Protozoan Parasites)
Cryptosporidium is an apicomplexan parasitic protist, which infects a wide range of hosts, causing cryptosporidiosis disease. In farms, the incidence of this disease is high in animals such as cows, leading to extensive economic loss in the livestock industry. Infected cows may also act as a major reservoir of Cryptosporidium spp., in particular C. parvum, the most common cause of cryptosporidiosis in these animals. This poses a risk to the trading of livestock, to other farms via breeding centres, and to human health. This study is a part of a global project aimed at strategies to tackle cryptosporidiosis. To reach this target, it was essential to determine whether prevalence was dependent on the studied countries or if the issue was borderless. Indeed, C. parvum occurrence was assessed across dairy farms in certain regions of Belgium, France, and the Netherlands. At the same time, the animal-to-animal transmission of the circulating C. parvum subtypes was studied. To accomplish this, we analysed 1084 faecal samples, corresponding to 57 dairy farms from all three countries. To this end, 18S rRNA and gp60 genes fragments were amplified, followed by DNA sequencing, which was subsequently used for detection and subtyping C. parvum. Bioinformatic and phylogenetic methods were integrated to analyse and characterise the obtained DNA sequences. Our results show 25.7%, 24.9% and 20.8% prevalence of Cryptosporidium spp. in Belgium, France, and the Netherlands respectively. Overall, 93% of the farms were Cryptosporidium positive. The gp60 subtyping demonstrated a significant number of the C. parvum positives belonged to the IIa allelic family, which has been also identified in humans. Therefore, this study highlights how prevalent C. parvum is in dairy farms and further suggests cattle as a possible carrier of zoonotic C. parvum subtypes, which could pose a threat to human health. View Full-Text
Keywords: 18S rRNA; Cryptosporidium; dairy cattle; gp60; genotyping; prevalence 18S rRNA; Cryptosporidium; dairy cattle; gp60; genotyping; prevalence
Show Figures

Figure 1

MDPI and ACS Style

Pinto, P.; Ribeiro, C.A.; Hoque, S.; Hammouma, O.; Leruste, H.; Détriché, S.; Canniere, E.; Daandels, Y.; Dellevoet, M.; Roemen, J.; Barbier Bourgeois, A.; Kváč, M.; Follet, J.; Tsaousis, A.D. Cross-Border Investigations on the Prevalence and Transmission Dynamics of Cryptosporidium Species in Dairy Cattle Farms in Western Mainland Europe. Microorganisms 2021, 9, 2394. https://doi.org/10.3390/microorganisms9112394

AMA Style

Pinto P, Ribeiro CA, Hoque S, Hammouma O, Leruste H, Détriché S, Canniere E, Daandels Y, Dellevoet M, Roemen J, Barbier Bourgeois A, Kváč M, Follet J, Tsaousis AD. Cross-Border Investigations on the Prevalence and Transmission Dynamics of Cryptosporidium Species in Dairy Cattle Farms in Western Mainland Europe. Microorganisms. 2021; 9(11):2394. https://doi.org/10.3390/microorganisms9112394

Chicago/Turabian Style

Pinto, Pedro, Cláudia A. Ribeiro, Sumaiya Hoque, Ourida Hammouma, Hélène Leruste, Sébastien Détriché, Evi Canniere, Yvonne Daandels, Martine Dellevoet, Janine Roemen, Anne Barbier Bourgeois, Martin Kváč, Jérôme Follet, and Anastasios D. Tsaousis 2021. "Cross-Border Investigations on the Prevalence and Transmission Dynamics of Cryptosporidium Species in Dairy Cattle Farms in Western Mainland Europe" Microorganisms 9, no. 11: 2394. https://doi.org/10.3390/microorganisms9112394

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop