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Dietary Contamination with a Neonicotinoid (Clothianidin) Gradient Triggers Specific Dysbiosis Signatures of Microbiota Activity along the Honeybee (Apis mellifera) Digestive Tract

1
Department of Biology, Laval University, Québec, QC G1V 0A6, Canada
2
Institut de Biologie Intégrative et des Systèmes (IBIS), Laval University, Québec, QC G1V 0A6, Canada
3
Institute of Biodiversity, Animal Health & Comparative Medicine, University of Glasgow, Glasgow G12 8QQ, UK
*
Author to whom correspondence should be addressed.
Academic Editor: Matthias Noll
Microorganisms 2021, 9(11), 2283; https://doi.org/10.3390/microorganisms9112283
Received: 22 September 2021 / Revised: 25 October 2021 / Accepted: 27 October 2021 / Published: 2 November 2021
(This article belongs to the Section Environmental Microbiology)
Pesticides are increasing honeybee (Apis mellifera) death rates globally. Clothianidin neonicotinoid appears to impair the microbe–immunity axis. We conducted cage experiments on newly emerged bees that were 4–6 days old and used a 16S rRNA metataxonomic approach to measure the impact of three sublethal clothianidin concentrations (0.1, 1 and 10 ppb) on survival, sucrose syrup consumption and gut microbiota community structure. Exposure to clothianidin significantly increased mortality in the three concentrations compared to controls. Interestingly, the lowest clothianidin concentration was associated with the highest mortality, and the medium concentration with the highest food intake. Exposure to clothianidin induced significant variation in the taxonomic distribution of gut microbiota activity. Co-abundance network analysis revealed local dysbiosis signatures specific to each gut section (midgut, ileum and rectum) were driven by specific taxa. Our findings confirm that exposure to clothianidin triggers a reshuffling of beneficial strains and/or potentially pathogenic taxa within the gut, suggesting a honeybee’s symbiotic defense systems’ disruption, such as resistance to microbial colonization. This study highlights the role of weak transcriptional activity taxa in maintaining a stable honeybee gut microbiota. Finally, the early detection of gut dysbiosis in honeybees is a promising biomarker in hive management for assessing the impact exposure to sublethal xenobiotics. View Full-Text
Keywords: honeybee; clothianidin; microbiota; dysbiosis; network analysis honeybee; clothianidin; microbiota; dysbiosis; network analysis
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MDPI and ACS Style

El Khoury, S.; Gauthier, J.; Bouslama, S.; Cheaib, B.; Giovenazzo, P.; Derome, N. Dietary Contamination with a Neonicotinoid (Clothianidin) Gradient Triggers Specific Dysbiosis Signatures of Microbiota Activity along the Honeybee (Apis mellifera) Digestive Tract. Microorganisms 2021, 9, 2283. https://doi.org/10.3390/microorganisms9112283

AMA Style

El Khoury S, Gauthier J, Bouslama S, Cheaib B, Giovenazzo P, Derome N. Dietary Contamination with a Neonicotinoid (Clothianidin) Gradient Triggers Specific Dysbiosis Signatures of Microbiota Activity along the Honeybee (Apis mellifera) Digestive Tract. Microorganisms. 2021; 9(11):2283. https://doi.org/10.3390/microorganisms9112283

Chicago/Turabian Style

El Khoury, Sarah, Jeff Gauthier, Sidki Bouslama, Bachar Cheaib, Pierre Giovenazzo, and Nicolas Derome. 2021. "Dietary Contamination with a Neonicotinoid (Clothianidin) Gradient Triggers Specific Dysbiosis Signatures of Microbiota Activity along the Honeybee (Apis mellifera) Digestive Tract" Microorganisms 9, no. 11: 2283. https://doi.org/10.3390/microorganisms9112283

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