Identification of an Acidic Amino Acid Permease Involved in d-Aspartate Uptake in the Yeast Cryptococcus humicola
Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan
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Academic Editor: Peter Richard
Microorganisms 2021, 9(1), 192; https://doi.org/10.3390/microorganisms9010192
Received: 14 December 2020
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Revised: 4 January 2021
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Accepted: 14 January 2021
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Published: 18 January 2021
(This article belongs to the Section Molecular Microbiology and Immunology)
d-aspartate oxidase (DDO) catalyzes the oxidative deamination of acidic d-amino acids, and its production is induced by d-Asp in several eukaryotes. The yeast Cryptococcus humicola strain UJ1 produces large amounts of DDO (ChDDO) only in the presence of d-Asp. In this study, we analyzed the relationship between d-Asp uptake by an amino acid permease (Aap) and the inducible expression of ChDDO. We identified two acidic Aap homologs, named “ChAap4 and ChAap5,” in the yeast genome sequence. ChAAP4 deletion resulted in partial growth defects on d-Asp as well as l-Asp, l-Glu, and l-Phe at pH 7, whereas ChAAP5 deletion caused partial growth defects on l-Phe and l-Lys, suggesting that ChAap4 might participate in d-Asp uptake as an acidic Aap. Interestingly, the growth of the Chaap4 strain on d- or l-Asp was completely abolished at pH 10, suggesting that ChAap4 is the only Aap responsible for d- and l-Asp uptake under high alkaline conditions. In addition, ChAAP4 deletion significantly decreased the induction of DDO activity and ChDDO transcription in the presence of d-Asp. This study revealed that d-Asp uptake by ChAap4 might be involved in the induction of ChDDO expression by d-Asp.
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Keywords:
d-aspartate oxidase; amino acid permease; Cryptococcus humicola; d-aspartate; gene expression
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MDPI and ACS Style
Imanishi, D.; Kera, Y.; Takahashi, S. Identification of an Acidic Amino Acid Permease Involved in d-Aspartate Uptake in the Yeast Cryptococcus humicola. Microorganisms 2021, 9, 192. https://doi.org/10.3390/microorganisms9010192
AMA Style
Imanishi D, Kera Y, Takahashi S. Identification of an Acidic Amino Acid Permease Involved in d-Aspartate Uptake in the Yeast Cryptococcus humicola. Microorganisms. 2021; 9(1):192. https://doi.org/10.3390/microorganisms9010192
Chicago/Turabian StyleImanishi, Daiki, Yoshio Kera, and Shouji Takahashi. 2021. "Identification of an Acidic Amino Acid Permease Involved in d-Aspartate Uptake in the Yeast Cryptococcus humicola" Microorganisms 9, no. 1: 192. https://doi.org/10.3390/microorganisms9010192
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