First Cases of Natural Infections with Borrelia hispanica in Two Dogs and a Cat from Europe
Abstract
:1. Background
2. Case Reports
2.1. Canine Case 1 (Dog 1; Sample ID VM531519)
2.2. Canine Case 2 (Dog 2; Sample ID VM736940)
2.3. Feline Case (Cat; Sample ID 10827448)
3. Methods
3.1. Samples Included in Our Study
3.2. Serology IDEXX Ludwigsburg, Germany
3.3. PCRs IDEXX Ludwigsburg, Germany
3.4. In Vitro Culture from Cat Blood at NRZ Borrelia, Oberschleißheim, Germany
3.5. DNA Extraction and PCR (NRZ Borrelia, Oberschleißheim)
3.6. Molecular Analyses
3.7. Sequence Deposition
4. Results and Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Sample/Date | Dog 1 | Dog 2 | Cat |
---|---|---|---|
Date of sampling | 30 October 2014 | 21 June 2018 | 21 July 2018 |
Lab sample ID | VM531519/10015666 | VM73694/11024511 | 10827448 |
Clinical signs | Lethargic | Lethargic | Cachexia |
Pale mucosa, hematocrit 30.2% (reference range 38.3–56.5%) | High body temperature (39 °C) | Abdominal respiration | |
Clinico-pathological abnormalities | Non-regenerative anemia; (erythrocytes 3.98 m/µL, hemoglobin 8.9 g/dL, hematocrit 29.4%, reticulocytes 58,108/µL) | Leukocytosis (20.3 g/L) | Regenerative anemia (low erythrocytes 2.45 m/µL, hemoglobin 3.3 g/dL, and hematocrit 12.9%; hematocrit reference range 25–45%) |
Thrombocytopenia (23 tsd.) | Thrombocytopenia (92 tsd.) | High reticulocytes numbers (139,895/µL); mild monocytosis | |
Blood taken | February 2014 March 2014 October 2014 | June 2018 | July 2018 |
Blood smear | Borrelia positive (second presentation) | Borrelia positive (very few bacteria) Babesia spp. positive (low level) | Borrelia positive |
Previous treatment | Doxycycline (2 courses; Feb 2014 and March 2014) | None | None |
Locus | Primer Name | Primer Sequence |
---|---|---|
clpA | clpAF1258 | 5′-GATAAAGCTTTTGAYYTATTAGATGG-3 |
clpA | clpAR2276 | 5′-TCATATTTDATRGTDTCGTC-3′ |
clpX | clpXF104 | 5′-CTGTTGCYATTTGTTTTGAATGYTC-3′ |
clpX | clpXR1277 | 5′-TAAAGTTCTTTTGCCCAAGG-3′ |
pepX | pepF361 | 5′-AGAGAYTTAAGYTTAKCAGG-3′ |
pepX | pepR1207 | 5′-CYATAGTTTCTCTTAAAGAYTGC-3 |
pyrG | pyrF379 | 5′-TATTTAGGKAGAACTGTACAGC-3 |
pyrG | pyrR1375 | 5′-CAAGTCGCATTGTWGCAC-3 |
recG | recF898 | 5′-GCKTTTCTMTCTAGYATTCC-3 |
recG | recR1779 | 5′-TTCRGTTAAAGGTTCCTTATAAAG-3 |
rplB | rplF3 | 5′-GGAGAAAAATATGGGKATTAAGAC-3 |
rplB | rplR769 | 5′-GRCCCCAAGGWGATAC-3 |
uvrA | uvrF1170 | 5′-GAGGCGTTATCTTWCAAC-3 |
uvrA | uvrR2181 | 5′-AGACTCTGGAAGCTTWGC-3 |
Locus/Sample | Species and Isolate | Coverage | Similarity | GenBank Accession Number |
---|---|---|---|---|
clpX/dog1 (#allele 210)/cat (#allele 210) | Borrelia crocidurae str. Achema | 100% | 97% | CP003426.1 |
Borrelia recurrentis A1 | 100% | 97% | CP000993.1 | |
Borrelia duttonii Ly | 100% | 97% | CP000976.1 | |
Borrelia crocidurae DOU | 100% | 96% | CP004267.1 | |
Borrelia persica strain LMU-T01 | 99% | 91% | KP826804. | |
pepX/cat (#allele 263) | Borrelia recurrentis A1 | 100% | 98% | CP000993.1 |
Borrelia crocidurae DOU | 100% | 97% | CP004267.1 | |
Borrelia crocidurae str. Achema | 100% | 97% | CP003426.1 | |
Borrelia duttonii Ly | 99% | 97% | CP000976.1 | |
Borrelia persica strain LMU-T01 | 100% | 90% | KP826805.1 | |
pyrG/dog1 (#allele 274) | Borrelia crocidurae str. Achema | 100% | 98% | CP003426.1 |
Borrelia recurrentis A1 | 100% | 97% | CP000993.1 | |
Borrelia duttonii Ly | 100% | 97% | CP000976.1 | |
Borrelia persica strain LMU-T01 | 100% | 89% | KP826806.1 | |
pyrG/cat (#allele 273) | Borrelia crocidurae DOU | 100% | 98% | CP004267.1 |
Borrelia crocidurae str. Achema | 100% | 98% | CP003426.1 | |
Borrelia recurrentis A1 | 100% | 98% | CP000993.1 | |
Borrelia duttonii Ly | 100% | 98% | CP000976.1 | |
Borrelia persica strain LMU-T01 | 100% | 90% | KP826806.1 | |
recG/cat (#allele 288) | Borrelia crocidurae str. Achema | 100% | 97% | CP003426.1 |
Borrelia crocidurae DOU | 100% | 97% | CP004267.1 | |
Borrelia duttonii Ly | 100% | 97% | CP000976.1 | |
Borrelia recurrentis A1 | 100% | 96% | CP000993.1 | |
Borrelia persica strain LMU-T01 | 99% | 89% | KP826807.1 | |
rplB/dog1 (#allele 206) | Borrelia crocidurae str. Achema | 100% | 98% | CP003426.1 |
Borrelia recurrentis A1 | 100% | 98% | CP000993.1 | |
Borrelia duttonii Ly | 100% | 98% | CP000976.1 | |
Borrelia crocidurae DOU | 100% | 98% | CP004267.1 | |
Borrelia persica strain LMU-T01 | 98% | 92% | KP826807.1 | |
16S rRNA/dog2 (* MN175320)/cat (* MN173954) | Borrelia hispanica strain CR1 | 100% | 100% | GU350705.1 |
Borrelia hispanica 16S rRNA gene | 100% | 100% | DQ057988.1 | |
Borrelia crocidurae str. Achema | 100% | 99% | CP003426.1 | |
Borrelia hispanica strain Sp3 | 100% | 99% | GU350706.1 | |
Borrelia crocidurae strain 7-10TO58 | 100% | 99% | GQ358198.1 |
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Margos, G.; Pantchev, N.; Globokar, M.; Lopez, J.; Rodon, J.; Hernandez, L.; Herold, H.; Salas, N.; Civit, A.; Fingerle, V. First Cases of Natural Infections with Borrelia hispanica in Two Dogs and a Cat from Europe. Microorganisms 2020, 8, 1251. https://doi.org/10.3390/microorganisms8081251
Margos G, Pantchev N, Globokar M, Lopez J, Rodon J, Hernandez L, Herold H, Salas N, Civit A, Fingerle V. First Cases of Natural Infections with Borrelia hispanica in Two Dogs and a Cat from Europe. Microorganisms. 2020; 8(8):1251. https://doi.org/10.3390/microorganisms8081251
Chicago/Turabian StyleMargos, Gabriele, Nikola Pantchev, Majda Globokar, Javier Lopez, Jaume Rodon, Leticia Hernandez, Heike Herold, Noelia Salas, Anna Civit, and Volker Fingerle. 2020. "First Cases of Natural Infections with Borrelia hispanica in Two Dogs and a Cat from Europe" Microorganisms 8, no. 8: 1251. https://doi.org/10.3390/microorganisms8081251
APA StyleMargos, G., Pantchev, N., Globokar, M., Lopez, J., Rodon, J., Hernandez, L., Herold, H., Salas, N., Civit, A., & Fingerle, V. (2020). First Cases of Natural Infections with Borrelia hispanica in Two Dogs and a Cat from Europe. Microorganisms, 8(8), 1251. https://doi.org/10.3390/microorganisms8081251