Lactobacillus Mucosae Strain Promoted by a High-Fiber Diet in Genetic Obese Child Alleviates Lipid Metabolism and Modifies Gut Microbiota in ApoE-/- Mice on a Western Diet
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sequence-Guided Isolation and Identification of A1 strain
2.2. Whole-Genome Sequencing and Analysis of A1 Strain
2.3. Culture and Administration of A1 Strain
2.4. Animal Trial and Sample Collection
2.5. Histopathology of Epididymal Adipose Tissue (eAT), Liver and Aortic Sinus
2.6. Hepatic and Serum Lipid Measurement
2.7. Serum TMA and TMAO Measurement
2.8. Serum LBP Measurement
2.9. Statistical Analysis for Animal Trial
2.10. Gut Microbiota Profiling
2.11. Accession Numbers for Sequence Data
3. Results
3.1. Isolation of Human-Derived L. Mucosae A1 Enriched by a High-Fiber Dietary Intervention
3.2. L. mucosae A1 Alleviated Severe Lipid Accumulation in ApoE-/- Mice on a Western Diet
3.3. L. mucosae A1 Protected Western Diet-Fed ApoE-/- Mice from Atherosclerosis
3.4. L. mucosae A1 Modulated the Gut Microbiota in ApoE-/- Mice on a Western Diet
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Jiang, T.; Wu, H.; Yang, X.; Li, Y.; Zhang, Z.; Chen, F.; Zhao, L.; Zhang, C. Lactobacillus Mucosae Strain Promoted by a High-Fiber Diet in Genetic Obese Child Alleviates Lipid Metabolism and Modifies Gut Microbiota in ApoE-/- Mice on a Western Diet. Microorganisms 2020, 8, 1225. https://doi.org/10.3390/microorganisms8081225
Jiang T, Wu H, Yang X, Li Y, Zhang Z, Chen F, Zhao L, Zhang C. Lactobacillus Mucosae Strain Promoted by a High-Fiber Diet in Genetic Obese Child Alleviates Lipid Metabolism and Modifies Gut Microbiota in ApoE-/- Mice on a Western Diet. Microorganisms. 2020; 8(8):1225. https://doi.org/10.3390/microorganisms8081225
Chicago/Turabian StyleJiang, Tianyi, Huan Wu, Xin Yang, Yue Li, Ziyi Zhang, Feng Chen, Liping Zhao, and Chenhong Zhang. 2020. "Lactobacillus Mucosae Strain Promoted by a High-Fiber Diet in Genetic Obese Child Alleviates Lipid Metabolism and Modifies Gut Microbiota in ApoE-/- Mice on a Western Diet" Microorganisms 8, no. 8: 1225. https://doi.org/10.3390/microorganisms8081225