Contribution of Hydrogen Cyanide to the Antagonistic Activity of Pseudomonas Strains Against Phytophthora infestans
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains and Culture Media
2.2. Generation of Δhcn Strains
2.3. Growth Curves
2.4. Cyanide Detection Assay
2.5. Dual Assays
2.6. Zoospore Germination Experiment
2.7. Leaf Disk Experiment
2.8. Fatty Acid Methyl Esters Analysis (FAMEs)
3. Results
3.1. Contribution of HCN to the Inhibition of P. infestans Mycelium Growth
3.2. Contribution of HCN to the Inhibition of P. infestans Zoospore Germination
3.3. Contribution of HCN to the Inhibition of Infection by P. infestans on Potato Leaf Disks
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Anand, A.; Chinchilla, D.; Tan, C.; Mène-Saffrané, L.; L’Haridon, F.; Weisskopf, L. Contribution of Hydrogen Cyanide to the Antagonistic Activity of Pseudomonas Strains Against Phytophthora infestans . Microorganisms 2020, 8, 1144. https://doi.org/10.3390/microorganisms8081144
Anand A, Chinchilla D, Tan C, Mène-Saffrané L, L’Haridon F, Weisskopf L. Contribution of Hydrogen Cyanide to the Antagonistic Activity of Pseudomonas Strains Against Phytophthora infestans . Microorganisms. 2020; 8(8):1144. https://doi.org/10.3390/microorganisms8081144
Chicago/Turabian StyleAnand, Abhishek, Delphine Chinchilla, Christopher Tan, Laurent Mène-Saffrané, Floriane L’Haridon, and Laure Weisskopf. 2020. "Contribution of Hydrogen Cyanide to the Antagonistic Activity of Pseudomonas Strains Against Phytophthora infestans " Microorganisms 8, no. 8: 1144. https://doi.org/10.3390/microorganisms8081144