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Isolation, Characterization, and Pathogenicity of Two Pseudomonas syringae Pathovars from Populus trichocarpa Seeds

1
Bioscience Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA
2
Geologic and Environmental Systems Directorate, National Energy Technology Laboratory, Pittsburgh, PA 15236, USA
3
Department of Forest, Rangeland and Fire Sciences, University of Idaho, Moscow, ID 83844, USA
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(8), 1137; https://doi.org/10.3390/microorganisms8081137
Received: 2 June 2020 / Revised: 21 July 2020 / Accepted: 23 July 2020 / Published: 28 July 2020
(This article belongs to the Section Plant Microbe Interactions)
Pseudomonas syringae is a ubiquitous plant pathogen, infecting both woody and herbaceous plants and resulting in devastating agricultural crop losses. Characterized by a remarkable specificity for plant hosts, P. syringae pathovars utilize a number of virulence factors including the type III secretion system and effector proteins to elicit disease in a particular host species. Here, two Pseudomonas syringae strains were isolated from diseased Populustrichocarpa seeds. The pathovars were capable of inhibiting poplar seed germination and were selective for the Populus genus. Sequencing of the newly described organisms revealed similarity to phylogroup II pathogens and genomic regions associated with woody host-associated plant pathogens, as well as genes for specific virulence factors. The host response to infection, as revealed through metabolomics, is the induction of the stress response through the accumulation of higher-order salicylates. Combined with necrosis on leaf surfaces, the plant appears to quickly respond by isolating infected tissues and mounting an anti-inflammatory defense. This study improves our understanding of the initial host response to epiphytic pathogens in Populus and provides a new model system for studying the effects of a bacterial pathogen on a woody host plant in which both organisms are fully genetically sequenced. View Full-Text
Keywords: Pseudomonas; virulence; pathogen; metabolomics; germination Pseudomonas; virulence; pathogen; metabolomics; germination
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MDPI and ACS Style

Saint-Vincent, P.M.; Ridout, M.; Engle, N.L.; Lawrence, T.J.; Yeary, M.L.; Tschaplinski, T.J.; Newcombe, G.; Pelletier, D.A. Isolation, Characterization, and Pathogenicity of Two Pseudomonas syringae Pathovars from Populus trichocarpa Seeds. Microorganisms 2020, 8, 1137. https://doi.org/10.3390/microorganisms8081137

AMA Style

Saint-Vincent PM, Ridout M, Engle NL, Lawrence TJ, Yeary ML, Tschaplinski TJ, Newcombe G, Pelletier DA. Isolation, Characterization, and Pathogenicity of Two Pseudomonas syringae Pathovars from Populus trichocarpa Seeds. Microorganisms. 2020; 8(8):1137. https://doi.org/10.3390/microorganisms8081137

Chicago/Turabian Style

Saint-Vincent, Patricia M., Mary Ridout, Nancy L. Engle, Travis J. Lawrence, Meredith L. Yeary, Timothy J. Tschaplinski, George Newcombe, and Dale A. Pelletier 2020. "Isolation, Characterization, and Pathogenicity of Two Pseudomonas syringae Pathovars from Populus trichocarpa Seeds" Microorganisms 8, no. 8: 1137. https://doi.org/10.3390/microorganisms8081137

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