A Multistage Formulation Based on Full-Length CSP and AMA-1 Ectodomain of Plasmodium vivax Induces High Antibody Titers and T-cells and Partially Protects Mice Challenged with a Transgenic Plasmodium berghei Parasite
Abstract
:1. Introduction
2. Materials and Methods
2.1. Recombinant Protein Expressed in Pichia Pastoris
2.2. Recombinant Proteins Expressed in Escherichia coli
2.3. Animals
2.4. Analysis of IgG Antibody by ELISA
2.5. Indirect Immunofluorescence Assay
2.6. Carboxyfluorescein Diacetate Succinimidyl Ester (CFSE)-Based Proliferation Assay
2.7. Parasites, Mice, and Mosquitoes
2.8. Transgenic P. berghei Sporozoite Challenge
2.9. Statistical Analysis
3. Results
3.1. Antibody Responses Induced by the Vaccine Formulations in BALB/c and C57BL/6 Mice
3.2. Cell-Mediated Immune Response
3.3. Recognition of the Native Protein PvCSP
3.4. Pb/PvCSP-VK210 Challenge
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Lima, L.C.; Marques, R.F.; Gimenez, A.M.; Françoso, K.S.; Aliprandini, E.; Camargo, T.M.; Aguiar, A.C.C.; Pereira, D.B.; Renia, L.; Amino, R.; et al. A Multistage Formulation Based on Full-Length CSP and AMA-1 Ectodomain of Plasmodium vivax Induces High Antibody Titers and T-cells and Partially Protects Mice Challenged with a Transgenic Plasmodium berghei Parasite. Microorganisms 2020, 8, 916. https://doi.org/10.3390/microorganisms8060916
Lima LC, Marques RF, Gimenez AM, Françoso KS, Aliprandini E, Camargo TM, Aguiar ACC, Pereira DB, Renia L, Amino R, et al. A Multistage Formulation Based on Full-Length CSP and AMA-1 Ectodomain of Plasmodium vivax Induces High Antibody Titers and T-cells and Partially Protects Mice Challenged with a Transgenic Plasmodium berghei Parasite. Microorganisms. 2020; 8(6):916. https://doi.org/10.3390/microorganisms8060916
Chicago/Turabian StyleLima, Luciana C., Rodolfo F. Marques, Alba Marina Gimenez, Katia S. Françoso, Eduardo Aliprandini, Tarsila M. Camargo, Anna Caroline C. Aguiar, Dhelio B. Pereira, Laurent Renia, Rogerio Amino, and et al. 2020. "A Multistage Formulation Based on Full-Length CSP and AMA-1 Ectodomain of Plasmodium vivax Induces High Antibody Titers and T-cells and Partially Protects Mice Challenged with a Transgenic Plasmodium berghei Parasite" Microorganisms 8, no. 6: 916. https://doi.org/10.3390/microorganisms8060916
APA StyleLima, L. C., Marques, R. F., Gimenez, A. M., Françoso, K. S., Aliprandini, E., Camargo, T. M., Aguiar, A. C. C., Pereira, D. B., Renia, L., Amino, R., & Soares, I. S. (2020). A Multistage Formulation Based on Full-Length CSP and AMA-1 Ectodomain of Plasmodium vivax Induces High Antibody Titers and T-cells and Partially Protects Mice Challenged with a Transgenic Plasmodium berghei Parasite. Microorganisms, 8(6), 916. https://doi.org/10.3390/microorganisms8060916