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Open AccessArticle

Importance of Defluviitalea raffinosedens for Hydrolytic Biomass Degradation in Co-Culture with Hungateiclostridium thermocellum

1
Chair of Microbiology, Technical University of Munich, Emil-Ramann-Str. 4, 85354 Freising, Germany
2
Department for Quality Assurance and Analytics, Bavarian State Research Center for Agriculture, Lange Point 6, 85354 Freising, Germany
3
Center for Biotechnology (CeBiTec), Universitätsstr. 27, 33615 Bielefeld, Germany
4
Faculty of Technology, Bielefeld University, Universitätsstr. 25, 33615 Bielefeld, Germany
5
Institute of Molecular Genetics, RAS, Kurchatov Sq. 2, 123182 Moscow, Russia
*
Authors to whom correspondence should be addressed.
These authors equally contributed to this work.
Microorganisms 2020, 8(6), 915; https://doi.org/10.3390/microorganisms8060915
Received: 19 May 2020 / Revised: 6 June 2020 / Accepted: 11 June 2020 / Published: 17 June 2020
(This article belongs to the Section Environmental Microbiology)
Bacterial hydrolysis of polysaccharides is an important step for the production of sustainable energy, for example during the conversion of plant biomass to methane-rich biogas. Previously, Hungateiclostridium thermocellum was identified as cellulolytic key player in thermophilic biogas microbiomes with a great frequency as an accompanying organism. The aim of this study was to physiologically characterize a recently isolated co-culture of H. thermocellum and the saccharolytic bacterium Defluviitalea raffinosedens from a laboratory-scale biogas fermenter. The characterization focused on cellulose breakdown by applying the measurement of cellulose hydrolysis, production of metabolites, and the activity of secreted enzymes. Substrate degradation and the production of volatile metabolites was considerably enhanced when both organisms acted synergistically. The metabolic properties of H. thermocellum have been studied well in the past. To predict the role of D. raffinosedens in this bacterial duet, the genome of D. raffinosedens was sequenced for the first time. Concomitantly, to deduce the prevalence of D. raffinosedens in anaerobic digestion, taxonomic composition and transcriptional activity of different biogas microbiomes were analyzed in detail. Defluviitalea was abundant and metabolically active in reactor operating at highly efficient process conditions, supporting the importance of this organism for the hydrolysis of the raw substrate. View Full-Text
Keywords: biogas; metagenomics; whole-genome sequencing; cellulose degradation; carbohydrate active enzymes; metabolic interaction biogas; metagenomics; whole-genome sequencing; cellulose degradation; carbohydrate active enzymes; metabolic interaction
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MDPI and ACS Style

Rettenmaier, R.; Schneider, M.; Munk, B.; Lebuhn, M.; Jünemann, S.; Sczyrba, A.; Maus, I.; Zverlov, V.; Liebl, W. Importance of Defluviitalea raffinosedens for Hydrolytic Biomass Degradation in Co-Culture with Hungateiclostridium thermocellum. Microorganisms 2020, 8, 915. https://doi.org/10.3390/microorganisms8060915

AMA Style

Rettenmaier R, Schneider M, Munk B, Lebuhn M, Jünemann S, Sczyrba A, Maus I, Zverlov V, Liebl W. Importance of Defluviitalea raffinosedens for Hydrolytic Biomass Degradation in Co-Culture with Hungateiclostridium thermocellum. Microorganisms. 2020; 8(6):915. https://doi.org/10.3390/microorganisms8060915

Chicago/Turabian Style

Rettenmaier, Regina; Schneider, Martina; Munk, Bernhard; Lebuhn, Michael; Jünemann, Sebastian; Sczyrba, Alexander; Maus, Irena; Zverlov, Vladimir; Liebl, Wolfgang. 2020. "Importance of Defluviitalea raffinosedens for Hydrolytic Biomass Degradation in Co-Culture with Hungateiclostridium thermocellum" Microorganisms 8, no. 6: 915. https://doi.org/10.3390/microorganisms8060915

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