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Article

Combined Effect of Naturally-Derived Biofilm Inhibitors and Differentiated HL-60 Cells in the Prevention of Staphylococcus aureus Biofilm Formation

1
Drug Research Program, Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, FI-00014 Helsinki, Finland
2
Department of Medical Microbiology and Infection Prevention, Amsterdam institute for Infection and Immunity, Amsterdam UMC, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands
3
Drug Research Program, Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, FI-00014 Helsinki, Finland
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(11), 1757; https://doi.org/10.3390/microorganisms8111757
Received: 12 October 2020 / Revised: 6 November 2020 / Accepted: 7 November 2020 / Published: 9 November 2020
(This article belongs to the Special Issue Staphylococcal Infections (Host and Pathogenic Factors))
Nosocomial diseases represent a huge health and economic burden. A significant portion is associated with the use of medical devices, with 80% of these infections being caused by a bacterial biofilm. The insertion of a foreign material usually elicits inflammation, which can result in hampered antimicrobial capacity of the host immunity due to the effort of immune cells being directed to degrade the material. The ineffective clearance by immune cells is a perfect opportunity for bacteria to attach and form a biofilm. In this study, we analyzed the antibiofilm capacity of three naturally derived biofilm inhibitors when combined with immune cells in order to assess their applicability in implantable titanium devices and low-density polyethylene (LDPE) endotracheal tubes. To this end, we used a system based on the coculture of HL-60 cells differentiated into polymorphonuclear leukocytes (PMNs) and Staphylococcus aureus (laboratory and clinical strains) on titanium, as well as LDPE surfaces. Out of the three inhibitors, the one coded DHA1 showed the highest potential to be incorporated into implantable devices, as it displayed a combined activity with the immune cells, preventing bacterial attachment on the titanium and LDPE. The other two inhibitors seemed to also be good candidates for incorporation into LDPE endotracheal tubes. View Full-Text
Keywords: Staphylococcus aureus; biomaterials; medical devices; HL-60 cells; PMNs; biofilm; endotracheal tube; titanium; implantable devices; nosocomial diseases Staphylococcus aureus; biomaterials; medical devices; HL-60 cells; PMNs; biofilm; endotracheal tube; titanium; implantable devices; nosocomial diseases
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MDPI and ACS Style

Reigada, I.; Guarch-Pérez, C.; Patel, J.Z.; Riool, M.; Savijoki, K.; Yli-Kauhaluoma, J.; Zaat, S.A.J.; Fallarero, A. Combined Effect of Naturally-Derived Biofilm Inhibitors and Differentiated HL-60 Cells in the Prevention of Staphylococcus aureus Biofilm Formation. Microorganisms 2020, 8, 1757. https://doi.org/10.3390/microorganisms8111757

AMA Style

Reigada I, Guarch-Pérez C, Patel JZ, Riool M, Savijoki K, Yli-Kauhaluoma J, Zaat SAJ, Fallarero A. Combined Effect of Naturally-Derived Biofilm Inhibitors and Differentiated HL-60 Cells in the Prevention of Staphylococcus aureus Biofilm Formation. Microorganisms. 2020; 8(11):1757. https://doi.org/10.3390/microorganisms8111757

Chicago/Turabian Style

Reigada, Inés, Clara Guarch-Pérez, Jayendra Z. Patel, Martijn Riool, Kirsi Savijoki, Jari Yli-Kauhaluoma, Sebastian A.J. Zaat, and Adyary Fallarero. 2020. "Combined Effect of Naturally-Derived Biofilm Inhibitors and Differentiated HL-60 Cells in the Prevention of Staphylococcus aureus Biofilm Formation" Microorganisms 8, no. 11: 1757. https://doi.org/10.3390/microorganisms8111757

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