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Communication

Bioactive Compounds of Nigella Sativa Essential Oil as Antibacterial Agents against Chlamydia Trachomatis D

1
Department of Medical Microbiology and Immunobiology, Faculty of Medicine, University of Szeged, 6720 Szeged, Hungary
2
Department of Microbiology and Biotechnology, Faculty of Food Science, Szent István University, 1118 Budapest, Hungary
*
Author to whom correspondence should be addressed.
Microorganisms 2019, 7(9), 370; https://doi.org/10.3390/microorganisms7090370
Received: 26 July 2019 / Revised: 13 September 2019 / Accepted: 18 September 2019 / Published: 19 September 2019
(This article belongs to the Special Issue Fighting Multidrug Resistance with Natural Antimicrobials)
Urogenital tract infection caused by obligate intracellular bacterium Chlamydia trachomatis D (CtrD) is a leading cause of sexually transmitted diseases. Essential oil (EO) of Nigella sativa has a broad antimicrobial spectrum. The aim of this study was to evaluate the antimicrobial activity of the bioactive compounds (p-cymene, thymoquinone, carvacrol, and thymol) of N. sativa EO against CtrD. The cytotoxic effects of the compounds were determined by MTT assay. In order to quantify the anti-chlamydial activity of the compounds, HeLa cells were infected with CtrD or CtrD treated previously with the compounds. The titer of the infectious CtrD was determined by indirect immunofluorescence assay. The minimum inhibitory concentrations of the compounds were evaluated by direct quantitative PCR. None of the compounds showed a cytotoxic effect on HeLa cells in the concentrations tested. According to the immunofluorescence assay, all of the compounds significantly inhibited the growth of CtrD. The quantitative PCR revealed that the minimum concentration that exerted anti-chlamydial activity was 3.12 µM in the case of thymoquinone and p-cymene, while that of carvacrol and thymol was 6.25 µM. Therefore, it can be concluded that bioactive compounds of N. sativa EO could be used as effective antimicrobial agents against CtrD. View Full-Text
Keywords: Chlamydia trachomatis; antibacterial activity; Nigella sativa Chlamydia trachomatis; antibacterial activity; Nigella sativa
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MDPI and ACS Style

Mosolygó, T.; Mouwakeh, A.; Hussein Ali, M.; Kincses, A.; Mohácsi-Farkas, C.; Kiskó, G.; Spengler, G. Bioactive Compounds of Nigella Sativa Essential Oil as Antibacterial Agents against Chlamydia Trachomatis D. Microorganisms 2019, 7, 370. https://doi.org/10.3390/microorganisms7090370

AMA Style

Mosolygó T, Mouwakeh A, Hussein Ali M, Kincses A, Mohácsi-Farkas C, Kiskó G, Spengler G. Bioactive Compounds of Nigella Sativa Essential Oil as Antibacterial Agents against Chlamydia Trachomatis D. Microorganisms. 2019; 7(9):370. https://doi.org/10.3390/microorganisms7090370

Chicago/Turabian Style

Mosolygó, Tímea, Ahmad Mouwakeh, Munira Hussein Ali, Annamária Kincses, Csilla Mohácsi-Farkas, Gabriella Kiskó, and Gabriella Spengler. 2019. "Bioactive Compounds of Nigella Sativa Essential Oil as Antibacterial Agents against Chlamydia Trachomatis D" Microorganisms 7, no. 9: 370. https://doi.org/10.3390/microorganisms7090370

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