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Microorganisms 2019, 7(3), 78; https://doi.org/10.3390/microorganisms7030078

Characterization a Novel Butyric Acid-Producing Bacterium Collinsella aerofaciens Subsp. Shenzhenensis Subsp. Nov.

1,2,3,*,†, 2,3,†, 2,3, 2,3, 2,3 and 2,3,*
1
BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China
2
BGI-Shenzhen, Shenzhen 518083, China
3
China National Genebank-Shenzhen, BGI-Shenzhen, Shenzhen 518120, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 22 January 2019 / Revised: 1 March 2019 / Accepted: 8 March 2019 / Published: 13 March 2019
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Abstract

Butyrate-producing bacteria can biosynthesize butyrate and alleviate inflammatory diseases. However, few studies have reported that the genus Collinsella has the ability to produce butyric acid. Here, our study depicts a Collinsella strain, which is a rod-shaped obligate anaerobe that is able to produce butyric acid. This microorganism was isolated from a human gut, and the optimal growth conditions were found to be 37 °C on PYG medium with pH 6.5. The 16S rRNA gene sequence demonstrated that this microorganism shared 99.93% similarity with C. aerofaciens ATCC 25986T, which was higher than the threshold (98.65%) for differentiating two species. Digital DNA–DNA hybridization and average nucleotide identity values also supported that this microorganism belonged to the species C. aerofaciens. Distinct phenotypic characteristics between TF06-26 and the type strain of C. aerofaciens, such as the fermentation of D-lactose, D-fructose and D-maltose, positive growth under pH 5 and 0.2% (w/v) cholate, suggested this strain was a novel subspecies. Comparative genome analysis revealed that butyric acid kinase and phosphate butyryltransferase enzymes were coded exclusively by this strain, indicating a specific butyric acid-producing function of this C. aerofaciens subspecies within the genus Collinsella. Thus, Collinsella aerofaciens subsp. shenzhenensis subsp. nov. was proposed, with set strain TF06-26T (=CGMCC 1.5216T = DSM 105138T) as the type strain. View Full-Text
Keywords: novel subspecies; Collinsella; phylogenetics; biochemistry; comparative genome novel subspecies; Collinsella; phylogenetics; biochemistry; comparative genome
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Qin, P.; Zou, Y.; Dai, Y.; Luo, G.; Zhang, X.; Xiao, L. Characterization a Novel Butyric Acid-Producing Bacterium Collinsella aerofaciens Subsp. Shenzhenensis Subsp. Nov.. Microorganisms 2019, 7, 78.

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