Binding of Helicobacter pylori to Human Gastric Mucins Correlates with Binding of TFF1
by
Ciara Dunne 1, Julie Naughton 2, Gina Duggan 1, Catherine Loughrey 3, Michelle Kilcoyne 3,4
, Lokesh Joshi 3, Stephen Carrington 5, Helen Earley 1, Steffen Backert 6, Catherine Robbe Masselot 7, Felicity E. B. May 8 and Marguerite Clyne 1,*
Ciara Dunne 1, Julie Naughton 2, Gina Duggan 1, Catherine Loughrey 3, Michelle Kilcoyne 3,4, Lokesh Joshi 3, Stephen Carrington 5, Helen Earley 1, Steffen Backert 6, Catherine Robbe Masselot 7, Felicity E. B. May 8 and Marguerite Clyne 1,*
1
School of Medicine and Conway Institute, University College Dublin, Belfield, Dublin 4, D04 V1W8, Ireland
2
School of Biological Sciences, Dublin Institute of Technology, Kevin St., Dublin 2, D08 NF82, Ireland
3
Glycoscience Group, National Centre for Biomedical Engineering Science, National University of Ireland Galway, Galway, H91 TK33, Ireland
4
Carbohydrate Signalling Group, Microbiology, School of Natural Sciences, National University of Ireland Galway, Galway, H91 TK33, Ireland
5
Veterinary Science Centre, University College Dublin, Belfield, Dublin 4, D04 V1W8, Ireland
6
Division of Microbiology, Department of Biology, Friedrich Alexander University Erlangen-Nuremberg, Staudtstr. 5, D-91058 Erlangen, Germany
7
Unité de Glycobiologie Structurale et Fonctionnelle, University Lille Nord de France, USTL, UGSF, IFR 147, CNRS, UMR 8576, F-59650 Villeneuve d’Ascq, France
8
Northern Institute for Cancer Research, University of Newcastle Upon Tyne, Newcastle Upon Tyne NE2 4HH, UK
*
Author to whom correspondence should be addressed.
Microorganisms 2018, 6(2), 44; https://doi.org/10.3390/microorganisms6020044
Received: 27 March 2018 / Revised: 23 April 2018 / Accepted: 1 May 2018 / Published: 18 May 2018
(This article belongs to the Special Issue Gut Bacteria-Mucus Interaction)
Helicobacter pylori binds to the gastric mucin, MUC5AC, and to trefoil factor, TFF1, which has been shown to interact with gastric mucin. We examined the interactions of TFF1 and H. pylori with purified gastrointestinal mucins from different animal species and from humans printed on a microarray platform to investigate whether TFF1 may play a role in locating H. pylori in gastric mucus. TFF1 bound almost exclusively to human gastric mucins and did not interact with human colonic mucins. There was a strong correlation between binding of TFF1 and H. pylori to human gastric mucins, and between binding of both TFF1 and H. pylori to gastric mucins with that of Griffonia simplicifolia lectin-II, which is specific for terminal non-reducing α- or β-linked N-acetyl-d-glucosamine. These results suggest that TFF1 may help to locate H. pylori in a discrete layer of gastric mucus and hence restrain their interactions with epithelial cells.
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Keywords:
Helicobacter pylori; TFF1; mucin; lectin
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MDPI and ACS Style
Dunne, C.; Naughton, J.; Duggan, G.; Loughrey, C.; Kilcoyne, M.; Joshi, L.; Carrington, S.; Earley, H.; Backert, S.; Robbe Masselot, C.; May, F.E.B.; Clyne, M. Binding of Helicobacter pylori to Human Gastric Mucins Correlates with Binding of TFF1. Microorganisms 2018, 6, 44.
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