Phenotypic Ultra-Rapid Antimicrobial Susceptibility Testing for Ceftazidime–Avibactam: In Support of Antimicrobial Stewardship
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains
2.2. FASTinov Assay for CZA Susceptibility Determination
2.3. Flow Cytometry Analysis
2.4. Internal Control of FASTinov Assay
2.5. Reference Method for CZA AST
2.6. Characterization of Resistant CZA Isolates
2.7. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Strain Details | Number of Strains | Disk Diffusion Phenotype | FC Phenotype | Carbapenemase Gene | Meropenem |
---|---|---|---|---|---|
MIC (mg/L) | |||||
K. pneumoniae | 2 | R | R | blaNDM-1 blaNDM-1 | >32 |
E. coli | 1 | R | R | 2 | |
E. coli | 1 | R | R | blaIMP | 1 |
E. cloacae | 2 | R | R | blaVIM-1 | 16–64 |
E. asburiae | 2 | R | R | blaVIM-1 | 4–8 |
E. asburiae | 1 | R | R | blaVIM-2 | 2 |
E. hormaechei | 2 | R | R | blaVIM-1 | 8–>64 |
K. pneumoniae | 1 | R | R | blaVIM-34 | 64 |
S. marcescens | 12 | R | R | blaVIM-1 | 8–>64 |
K. pneumoniae | 1 | R | R | blaKPC-62 | 4 |
K. pneumoniae | 2 | R | R | blaKPC-31 | 0.12–0.25 |
K. cryoscescens | 1 | R | R | blaNDM-1+KPC-2 | 8 |
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Martins-Oliveira, I.; Pérez-Viso, B.; Gomes, R.; Abreu, D.; Silva-Dias, A.; Cantón, R.; Pina-Vaz, C. Phenotypic Ultra-Rapid Antimicrobial Susceptibility Testing for Ceftazidime–Avibactam: In Support of Antimicrobial Stewardship. Microorganisms 2025, 13, 414. https://doi.org/10.3390/microorganisms13020414
Martins-Oliveira I, Pérez-Viso B, Gomes R, Abreu D, Silva-Dias A, Cantón R, Pina-Vaz C. Phenotypic Ultra-Rapid Antimicrobial Susceptibility Testing for Ceftazidime–Avibactam: In Support of Antimicrobial Stewardship. Microorganisms. 2025; 13(2):414. https://doi.org/10.3390/microorganisms13020414
Chicago/Turabian StyleMartins-Oliveira, Inês, Blanca Pérez-Viso, Rosário Gomes, David Abreu, Ana Silva-Dias, Rafael Cantón, and Cidália Pina-Vaz. 2025. "Phenotypic Ultra-Rapid Antimicrobial Susceptibility Testing for Ceftazidime–Avibactam: In Support of Antimicrobial Stewardship" Microorganisms 13, no. 2: 414. https://doi.org/10.3390/microorganisms13020414
APA StyleMartins-Oliveira, I., Pérez-Viso, B., Gomes, R., Abreu, D., Silva-Dias, A., Cantón, R., & Pina-Vaz, C. (2025). Phenotypic Ultra-Rapid Antimicrobial Susceptibility Testing for Ceftazidime–Avibactam: In Support of Antimicrobial Stewardship. Microorganisms, 13(2), 414. https://doi.org/10.3390/microorganisms13020414