Studies Using Mutant Strains of Azospirillum brasilense Reveal That Atmospheric Nitrogen Fixation and Auxin Production Are Light Dependent Processes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacteria Growth
2.2. Plant Growth
2.3. Acetylene Reduction Assay (ARA)
2.4. Microbial 59Fe Uptake and Metabolic Transformation
2.5. Luciferase Chemiluminescence ATP Assay
2.6. Spectrophotometric Auxin Assay
2.7. Bacteria 52Mn Uptake
2.8. Statistical Analysis
3. Results and Discussion
3.1. Light Dependencies of BNF and Its Supporting Processes in A. brasilense
3.2. Light Dependencies of Auxin Biosynthesis and Its Supporting Processes in A. brasilense
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Housh, A.B.; Noel, R.; Powell, A.; Waller, S.; Wilder, S.L.; Sopko, S.; Benoit, M.; Powell, G.; Schueller, M.J.; Ferrieri, R.A. Studies Using Mutant Strains of Azospirillum brasilense Reveal That Atmospheric Nitrogen Fixation and Auxin Production Are Light Dependent Processes. Microorganisms 2023, 11, 1727. https://doi.org/10.3390/microorganisms11071727
Housh AB, Noel R, Powell A, Waller S, Wilder SL, Sopko S, Benoit M, Powell G, Schueller MJ, Ferrieri RA. Studies Using Mutant Strains of Azospirillum brasilense Reveal That Atmospheric Nitrogen Fixation and Auxin Production Are Light Dependent Processes. Microorganisms. 2023; 11(7):1727. https://doi.org/10.3390/microorganisms11071727
Chicago/Turabian StyleHoush, Alexandra Bauer, Randi Noel, Avery Powell, Spenser Waller, Stacy L. Wilder, Stephanie Sopko, Mary Benoit, Garren Powell, Michael J. Schueller, and Richard A. Ferrieri. 2023. "Studies Using Mutant Strains of Azospirillum brasilense Reveal That Atmospheric Nitrogen Fixation and Auxin Production Are Light Dependent Processes" Microorganisms 11, no. 7: 1727. https://doi.org/10.3390/microorganisms11071727