Diversity, Taxonomic Novelty, and Encoded Functions of Salar de Ascotán Microbiota, as Revealed by Metagenome-Assembled Genomes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Site of Study and Sample Collection
2.2. DNA Purification and Quality Assessment
2.3. 16S rRNA Metabarcoding and Microbial Diversity Analyses
2.4. Illumina and Nanopore Shotgun DNA Sequencing
2.5. Shotgun Sequence Diversity Analyses
2.6. Hybrid Metagenome Assembly and MAGs Reconstruction, Annotation, and Functional Profiling
2.7. Phylogenetic Analyses
3. Results
3.1. Microbial Diversity in Salar de Ascotán’s Water, Sediment, and Soil, as Revealed by 16S rRNA Metabarcoding
3.2. Metagenomic Analyses and Captured Sequence Diversity
3.3. Reconstruction and Taxonomic Novelty of Bacterial and Archaeal Genomes from Salar de Ascotán
3.4. Predicted Metabolic Capabilities and Molecular Functions Encoded in the Salar de Ascotán’s MAGs
4. Discussion
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Parameter | Water | Sediment | Soil |
---|---|---|---|
Location | S 21°29′52.7″ WO 68°15′25.3″ | S 21°29′52.3″ WO 68°15′26.6″ | S 21°29′53.7″ WO 68°15′26.9″’ |
Elevation (m.a.s.l 1) | 3343 | 3350 | 3329 |
Environmental temperature (°C) | 23.1 | 20.5 | 20.5 |
Water temperature (°C) | 16 | 23.5 | - |
Pressure (HPa) | 651.9 | 651.3 | 651.3 |
Humidity (%) | 9.2 | 12.0 | 12.0 |
Soil or sediment temperature (°C) | - | 22.0 | 17.5 |
pH | 6.5 | 7.5 | 7.0 |
Water conductivity (mS) | 3.93 | - | - |
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Veloso, M.; Waldisperg, A.; Arros, P.; Berríos-Pastén, C.; Acosta, J.; Colque, H.; Varas, M.A.; Allende, M.L.; Orellana, L.H.; Marcoleta, A.E. Diversity, Taxonomic Novelty, and Encoded Functions of Salar de Ascotán Microbiota, as Revealed by Metagenome-Assembled Genomes. Microorganisms 2023, 11, 2819. https://doi.org/10.3390/microorganisms11112819
Veloso M, Waldisperg A, Arros P, Berríos-Pastén C, Acosta J, Colque H, Varas MA, Allende ML, Orellana LH, Marcoleta AE. Diversity, Taxonomic Novelty, and Encoded Functions of Salar de Ascotán Microbiota, as Revealed by Metagenome-Assembled Genomes. Microorganisms. 2023; 11(11):2819. https://doi.org/10.3390/microorganisms11112819
Chicago/Turabian StyleVeloso, Marcelo, Angie Waldisperg, Patricio Arros, Camilo Berríos-Pastén, Joaquín Acosta, Hazajem Colque, Macarena A. Varas, Miguel L. Allende, Luis H. Orellana, and Andrés E. Marcoleta. 2023. "Diversity, Taxonomic Novelty, and Encoded Functions of Salar de Ascotán Microbiota, as Revealed by Metagenome-Assembled Genomes" Microorganisms 11, no. 11: 2819. https://doi.org/10.3390/microorganisms11112819